Genetic Mapping and Evolutionary Analyses of the Black Grain Trait in Barley
Barley occupies the widest ecological area among the major cereal crops, thereby generating a high potential for adaptive genetic diversity against various environmental factors. Colored barley such as black grain barley has been suggested to result from environmental adaptation to biotic and abioti...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2018.01921/full |
| _version_ | 1811278617352601600 |
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| author | Zhoukai Long Zhoukai Long Yong Jia Cong Tan Xiao-Qi Zhang Tefera Angessa Sue Broughton Sharon Westcott Fei Dai Guoping Zhang Dongfa Sun Yanhao Xu Chengdao Li Chengdao Li |
| author_facet | Zhoukai Long Zhoukai Long Yong Jia Cong Tan Xiao-Qi Zhang Tefera Angessa Sue Broughton Sharon Westcott Fei Dai Guoping Zhang Dongfa Sun Yanhao Xu Chengdao Li Chengdao Li |
| author_sort | Zhoukai Long |
| collection | DOAJ |
| description | Barley occupies the widest ecological area among the major cereal crops, thereby generating a high potential for adaptive genetic diversity against various environmental factors. Colored barley such as black grain barley has been suggested to result from environmental adaptation to biotic and abiotic stresses. Using one double haploid population (433 lines), plus three F5 recombinant inbred line (RIL) populations (1,009 lines), the black lemma and pericarp (Blp) gene was mapped between two Insertion/deletion (Indel) markers MC_1570156 and MC_162350 with a physical distance of 0.807 Mb, containing 21 annotated genes in the mapped interval. Whole-genome re-sequencing was performed on two Tibetan wild barley lines (X1 and W1) with black grain phenotype. The probable candidate genes for Blp were discussed based on gene functional annotation and gene sequence variation analyses. Thirteen polymorphic Indel markers covering the target genetic region were used to analyze 178 barley accessions including 49 black husk entries. Genotype-based clustering analyses showed that the black landraces of different geographical background may have evolved from a single origin. Our study represents a significant improvement on the genetic mapping of Blp and would facilitate future study on the characterization of the genetic basis underlying this interesting agronomic trait. |
| first_indexed | 2024-04-13T00:37:53Z |
| format | Article |
| id | doaj.art-8e90c79100c7434aa905276c860a2e83 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T00:37:53Z |
| publishDate | 2019-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-8e90c79100c7434aa905276c860a2e832022-12-22T03:10:16ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2019-01-01910.3389/fpls.2018.01921431092Genetic Mapping and Evolutionary Analyses of the Black Grain Trait in BarleyZhoukai Long0Zhoukai Long1Yong Jia2Cong Tan3Xiao-Qi Zhang4Tefera Angessa5Sue Broughton6Sharon Westcott7Fei Dai8Guoping Zhang9Dongfa Sun10Yanhao Xu11Chengdao Li12Chengdao Li13Hubei Collaborative Innovation Centre for Grain Industry, Yangtze University, Jingzhou, ChinaWestern Barley Genetic Alliance, Murdoch University, Perth, WA, AustraliaWestern Barley Genetic Alliance, Murdoch University, Perth, WA, AustraliaWestern Barley Genetic Alliance, Murdoch University, Perth, WA, AustraliaWestern Barley Genetic Alliance, Murdoch University, Perth, WA, AustraliaWestern Barley Genetic Alliance, Murdoch University, Perth, WA, AustraliaDepartment of Agriculture and Food, Government of Western Australia, South Perth, WA, AustraliaDepartment of Agriculture and Food, Government of Western Australia, South Perth, WA, AustraliaCollege of Agriculture and Biotechnology, Zhejiang University, Hangzhou, ChinaCollege of Agriculture and Biotechnology, Zhejiang University, Hangzhou, ChinaCollege of Plant Science, Huazhong Agricultural University, Wuhan, ChinaHubei Collaborative Innovation Centre for Grain Industry, Yangtze University, Jingzhou, ChinaWestern Barley Genetic Alliance, Murdoch University, Perth, WA, AustraliaDepartment of Agriculture and Food, Government of Western Australia, South Perth, WA, AustraliaBarley occupies the widest ecological area among the major cereal crops, thereby generating a high potential for adaptive genetic diversity against various environmental factors. Colored barley such as black grain barley has been suggested to result from environmental adaptation to biotic and abiotic stresses. Using one double haploid population (433 lines), plus three F5 recombinant inbred line (RIL) populations (1,009 lines), the black lemma and pericarp (Blp) gene was mapped between two Insertion/deletion (Indel) markers MC_1570156 and MC_162350 with a physical distance of 0.807 Mb, containing 21 annotated genes in the mapped interval. Whole-genome re-sequencing was performed on two Tibetan wild barley lines (X1 and W1) with black grain phenotype. The probable candidate genes for Blp were discussed based on gene functional annotation and gene sequence variation analyses. Thirteen polymorphic Indel markers covering the target genetic region were used to analyze 178 barley accessions including 49 black husk entries. Genotype-based clustering analyses showed that the black landraces of different geographical background may have evolved from a single origin. Our study represents a significant improvement on the genetic mapping of Blp and would facilitate future study on the characterization of the genetic basis underlying this interesting agronomic trait.https://www.frontiersin.org/article/10.3389/fpls.2018.01921/fullbarleyblack lemma and pericarpgenetic mappingwild barleyevolutionenvironmental adaptation |
| spellingShingle | Zhoukai Long Zhoukai Long Yong Jia Cong Tan Xiao-Qi Zhang Tefera Angessa Sue Broughton Sharon Westcott Fei Dai Guoping Zhang Dongfa Sun Yanhao Xu Chengdao Li Chengdao Li Genetic Mapping and Evolutionary Analyses of the Black Grain Trait in Barley Frontiers in Plant Science barley black lemma and pericarp genetic mapping wild barley evolution environmental adaptation |
| title | Genetic Mapping and Evolutionary Analyses of the Black Grain Trait in Barley |
| title_full | Genetic Mapping and Evolutionary Analyses of the Black Grain Trait in Barley |
| title_fullStr | Genetic Mapping and Evolutionary Analyses of the Black Grain Trait in Barley |
| title_full_unstemmed | Genetic Mapping and Evolutionary Analyses of the Black Grain Trait in Barley |
| title_short | Genetic Mapping and Evolutionary Analyses of the Black Grain Trait in Barley |
| title_sort | genetic mapping and evolutionary analyses of the black grain trait in barley |
| topic | barley black lemma and pericarp genetic mapping wild barley evolution environmental adaptation |
| url | https://www.frontiersin.org/article/10.3389/fpls.2018.01921/full |
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