The Sensitization of Legionella pneumophila to Some Antibiotics by Reserpine and Anti-Legionella Effects of Different Benzofuranone Derivatives
Background: Legionella pneumophila is a dangerous pathogenic bacterium can cause serious infectious diseases especially in hospitalized immuno-compromised patients. This bacterium is shown to be resistant against different antibiotics. Resistance against a wide range of antibiotics is usually medi...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2015-10-01
|
| Series: | Journal of Medical Bacteriology |
| Subjects: | |
| Online Access: | https://jmb.tums.ac.ir/index.php/jmb/article/view/38 |
| _version_ | 1818807538310709248 |
|---|---|
| author | Mohsen Khaleghi Latifeh Navidpour Zargham Sepehrizadeh Pardis Nazari MohammadReza Pourmand Nasrin Samadi SeyedMojtaba Moosavian AhmadReza Shahverdi |
| author_facet | Mohsen Khaleghi Latifeh Navidpour Zargham Sepehrizadeh Pardis Nazari MohammadReza Pourmand Nasrin Samadi SeyedMojtaba Moosavian AhmadReza Shahverdi |
| author_sort | Mohsen Khaleghi |
| collection | DOAJ |
| description | Background: Legionella pneumophila is a dangerous pathogenic bacterium can cause serious infectious diseases especially in hospitalized immuno-compromised patients. This bacterium is shown to be resistant against different antibiotics. Resistance against a wide range of antibiotics is usually mediated by efflux pump in bacteria. Efflux pumps are proteinaceous transporters localized in the cytoplasmic membrane of all kinds of cells which excreted antibiotics outside the cells. However, synthesis of new anti-Legionella compounds or selection of resistant modulating agents are useful strategy to combat with L. pneumophilain the future.
Methods: In this study the antibacterial activity of some benzofuranone derivatives have been investigated by disk diffusion method against L. pneumophila. Also the sensitivity of this test strain was evaluated against 19 antibiotics and the combination effect of reserpine at a sub-inhibitory concentration was further studied with these antibiotics using disk diffusion method with some modifications.
Conclusion: Among the different synthetic compounds which were tested against L. pneumophila, the most antibacterial activity was observed for compounds 1j and 1m which contain hydroxyl and methoxy groups on the C-6 and C-7 positionsagainst L. pneumophila. To evaluate whether efflux pumps are active in L. pneumophila or not an efflux inhibitor (reserpine) was tested in combination of different antibiotics against this test strain. Reserpine significantly enhanced the antibacterial activities of kanamycin, nitrofurantoin, co-trimoxazole, erythromycin, ofloxacillin, gentamycin, rifampin, ciprofloxacin, nalidixic acid, minocycline, tobramycin, and amikacin against L. pneumophila which shows the resistances to these antibiotics are mediated by efflux system in this bacterium. |
| first_indexed | 2024-12-18T19:27:15Z |
| format | Article |
| id | doaj.art-8e9599c74e544c8ba92e95d5e2061e37 |
| institution | Directory Open Access Journal |
| issn | 2251-8649 2322-2581 |
| language | English |
| last_indexed | 2024-12-18T19:27:15Z |
| publishDate | 2015-10-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Journal of Medical Bacteriology |
| spelling | doaj.art-8e9599c74e544c8ba92e95d5e2061e372022-12-21T20:55:50ZengTehran University of Medical SciencesJournal of Medical Bacteriology2251-86492322-25812015-10-0121-236The Sensitization of Legionella pneumophila to Some Antibiotics by Reserpine and Anti-Legionella Effects of Different Benzofuranone DerivativesMohsen Khaleghi0Latifeh Navidpour1Zargham Sepehrizadeh2Pardis Nazari3MohammadReza Pourmand4Nasrin Samadi5SeyedMojtaba Moosavian6AhmadReza Shahverdi7Department of Pharmaceutical Biotechnology and Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, IR Iran.Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, IR Iran.Department of Pharmaceutical Biotechnology and Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, IR Iran.Department of Pharmaceutical Biotechnology and Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, IR Iran.Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran.Department of Drug and Food Control and Quality Assurance Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, IR Iran.Department of Microbiology, School of Medicine and Tropical Research Center, Ahwaz Jundishapour University of Medical Sciences, Ahwaz, IR Iran.Department of Pharmaceutical Biotechnology and Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, IR Iran.Background: Legionella pneumophila is a dangerous pathogenic bacterium can cause serious infectious diseases especially in hospitalized immuno-compromised patients. This bacterium is shown to be resistant against different antibiotics. Resistance against a wide range of antibiotics is usually mediated by efflux pump in bacteria. Efflux pumps are proteinaceous transporters localized in the cytoplasmic membrane of all kinds of cells which excreted antibiotics outside the cells. However, synthesis of new anti-Legionella compounds or selection of resistant modulating agents are useful strategy to combat with L. pneumophilain the future. Methods: In this study the antibacterial activity of some benzofuranone derivatives have been investigated by disk diffusion method against L. pneumophila. Also the sensitivity of this test strain was evaluated against 19 antibiotics and the combination effect of reserpine at a sub-inhibitory concentration was further studied with these antibiotics using disk diffusion method with some modifications. Conclusion: Among the different synthetic compounds which were tested against L. pneumophila, the most antibacterial activity was observed for compounds 1j and 1m which contain hydroxyl and methoxy groups on the C-6 and C-7 positionsagainst L. pneumophila. To evaluate whether efflux pumps are active in L. pneumophila or not an efflux inhibitor (reserpine) was tested in combination of different antibiotics against this test strain. Reserpine significantly enhanced the antibacterial activities of kanamycin, nitrofurantoin, co-trimoxazole, erythromycin, ofloxacillin, gentamycin, rifampin, ciprofloxacin, nalidixic acid, minocycline, tobramycin, and amikacin against L. pneumophila which shows the resistances to these antibiotics are mediated by efflux system in this bacterium.https://jmb.tums.ac.ir/index.php/jmb/article/view/38ReserpineLegionella pneumophilaAnti-Bacterial Agents |
| spellingShingle | Mohsen Khaleghi Latifeh Navidpour Zargham Sepehrizadeh Pardis Nazari MohammadReza Pourmand Nasrin Samadi SeyedMojtaba Moosavian AhmadReza Shahverdi The Sensitization of Legionella pneumophila to Some Antibiotics by Reserpine and Anti-Legionella Effects of Different Benzofuranone Derivatives Journal of Medical Bacteriology Reserpine Legionella pneumophila Anti-Bacterial Agents |
| title | The Sensitization of Legionella pneumophila to Some Antibiotics by Reserpine and Anti-Legionella Effects of Different Benzofuranone Derivatives |
| title_full | The Sensitization of Legionella pneumophila to Some Antibiotics by Reserpine and Anti-Legionella Effects of Different Benzofuranone Derivatives |
| title_fullStr | The Sensitization of Legionella pneumophila to Some Antibiotics by Reserpine and Anti-Legionella Effects of Different Benzofuranone Derivatives |
| title_full_unstemmed | The Sensitization of Legionella pneumophila to Some Antibiotics by Reserpine and Anti-Legionella Effects of Different Benzofuranone Derivatives |
| title_short | The Sensitization of Legionella pneumophila to Some Antibiotics by Reserpine and Anti-Legionella Effects of Different Benzofuranone Derivatives |
| title_sort | sensitization of legionella pneumophila to some antibiotics by reserpine and anti legionella effects of different benzofuranone derivatives |
| topic | Reserpine Legionella pneumophila Anti-Bacterial Agents |
| url | https://jmb.tums.ac.ir/index.php/jmb/article/view/38 |
| work_keys_str_mv | AT mohsenkhaleghi thesensitizationoflegionellapneumophilatosomeantibioticsbyreserpineandantilegionellaeffectsofdifferentbenzofuranonederivatives AT latifehnavidpour thesensitizationoflegionellapneumophilatosomeantibioticsbyreserpineandantilegionellaeffectsofdifferentbenzofuranonederivatives AT zarghamsepehrizadeh thesensitizationoflegionellapneumophilatosomeantibioticsbyreserpineandantilegionellaeffectsofdifferentbenzofuranonederivatives AT pardisnazari thesensitizationoflegionellapneumophilatosomeantibioticsbyreserpineandantilegionellaeffectsofdifferentbenzofuranonederivatives AT mohammadrezapourmand thesensitizationoflegionellapneumophilatosomeantibioticsbyreserpineandantilegionellaeffectsofdifferentbenzofuranonederivatives AT nasrinsamadi thesensitizationoflegionellapneumophilatosomeantibioticsbyreserpineandantilegionellaeffectsofdifferentbenzofuranonederivatives AT seyedmojtabamoosavian thesensitizationoflegionellapneumophilatosomeantibioticsbyreserpineandantilegionellaeffectsofdifferentbenzofuranonederivatives AT ahmadrezashahverdi thesensitizationoflegionellapneumophilatosomeantibioticsbyreserpineandantilegionellaeffectsofdifferentbenzofuranonederivatives AT mohsenkhaleghi sensitizationoflegionellapneumophilatosomeantibioticsbyreserpineandantilegionellaeffectsofdifferentbenzofuranonederivatives AT latifehnavidpour sensitizationoflegionellapneumophilatosomeantibioticsbyreserpineandantilegionellaeffectsofdifferentbenzofuranonederivatives AT zarghamsepehrizadeh sensitizationoflegionellapneumophilatosomeantibioticsbyreserpineandantilegionellaeffectsofdifferentbenzofuranonederivatives AT pardisnazari sensitizationoflegionellapneumophilatosomeantibioticsbyreserpineandantilegionellaeffectsofdifferentbenzofuranonederivatives AT mohammadrezapourmand sensitizationoflegionellapneumophilatosomeantibioticsbyreserpineandantilegionellaeffectsofdifferentbenzofuranonederivatives AT nasrinsamadi sensitizationoflegionellapneumophilatosomeantibioticsbyreserpineandantilegionellaeffectsofdifferentbenzofuranonederivatives AT seyedmojtabamoosavian sensitizationoflegionellapneumophilatosomeantibioticsbyreserpineandantilegionellaeffectsofdifferentbenzofuranonederivatives AT ahmadrezashahverdi sensitizationoflegionellapneumophilatosomeantibioticsbyreserpineandantilegionellaeffectsofdifferentbenzofuranonederivatives |