Quantitative Polymerase Chain Reaction Coupled With Sodium Dodecyl Sulfate and Propidium Monoazide for Detection of Viable Streptococcus agalactiae in Milk

Quantitative Polymerase Chain Reaction Coupled With Sodium Dodecyl Sulfate and Propidium Monoazide for Detection of Viable Streptococcus agalactiae in Milk

Streptococcus agalactiae is an important pathogen causing bovine mastitis. The aim of this study was to develop a simple and specific method for direct detection of S. agalactiae from milk products. Propidium monoazide (PMA) and sodium dodecyl sulfate (SDS) were utilized to eliminate the interferenc...

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Bibliographic Details
Main Authors: Yankun Zhao, He Chen, Huimin Liu, Jianxing Cai, Lu Meng, Lei Dong, Nan Zheng, Jiaqi Wang, Cheng Wang
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-03-01
Series:Frontiers in Microbiology
Subjects:
Streptococcus agalactiae
propidium monoazide
sodium dodecyl sulfate
qPCR
milk
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2019.00661/full

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