To determine the sensitivity and specificity of Polymerase chain reaction (PCR) in the diagnosis of visceral leishmaniasis
<p><strong> </strong></p><p><strong>Introduction:</strong> Leishmaniasis is a disease caused by a protozoan parasite of the genus <em>Leishmania,</em> which is transmitted through bites of infected sandflies. It has been reported that the polymer...
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| Format: | Article |
| Language: | English |
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Sri Lankan Society for Microbiology
2014-02-01
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| Series: | Sri Lankan Journal of Infectious Diseases |
| Subjects: | |
| Online Access: | https://sljid.sljol.info/articles/6036 |
| _version_ | 1828199326590959616 |
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| author | MS ANJUM U ANSARI F HUSSAIN N KALSOOM |
| author_facet | MS ANJUM U ANSARI F HUSSAIN N KALSOOM |
| author_sort | MS ANJUM |
| collection | DOAJ |
| description | <p><strong> </strong></p><p><strong>Introduction:</strong> Leishmaniasis is a disease caused by a protozoan parasite of the genus <em>Leishmania,</em> which is transmitted through bites of infected sandflies. It has been reported that the polymerase chain reaction (PCR) is a more sensitive and specific test for the diagnosis of visceral leishmaniasis than bone marrow examination. This recent study is a renewed effort to validate the role of PCR in the diagnosis of visceral leishmaniasis.</p> <p><strong> </strong></p> <p><strong>Objective:</strong> The objective of this study was to determine the sensitivity and specificity of PCR in the diagnosis of visceral leishmaniasis.</p> <p><strong> </strong></p> <p><strong>Study design:</strong> Cross sectional (validation) study carried out in the Haematology department, Armed Forces Institute of Pathology, Rawalpindi. from 25<sup>th</sup> March 2011 to 24<sup>th</sup> March 2012.</p> <p><strong> </strong></p> <p><strong>Subjects and Methods:</strong> A total number of 59 patients with visceral leishmaniasis diagnosed on microscopic bone marrow examination with equal number of negative controls were studied. The subjects were tested for the presence of visceral leishmaniasis by the polymerase chain reaction.</p> <p><strong> </strong></p> <p><strong>Results:</strong> All the 59 patients were found to be positive for visceral leishmaniasis by PCR. None of the negative controls were positive by PCR.</p> <p><strong> </strong></p> <p><strong>Conclusion:</strong> The study validates that PCR is equal to microscopic bone marrow examination in the diagnosis of visceral leishmaniasis.</p><p> </p><p>DOI: <a href="http://dx.doi.org/10.4038/sljid.v4i1.6036">http://dx.doi.org/10.4038/sljid.v4i1.6036</a></p> |
| first_indexed | 2024-04-12T10:53:33Z |
| format | Article |
| id | doaj.art-8e9740e3d2504a9eb089ecca237603ca |
| institution | Directory Open Access Journal |
| issn | 2012-8169 2448-9654 |
| language | English |
| last_indexed | 2024-04-12T10:53:33Z |
| publishDate | 2014-02-01 |
| publisher | Sri Lankan Society for Microbiology |
| record_format | Article |
| series | Sri Lankan Journal of Infectious Diseases |
| spelling | doaj.art-8e9740e3d2504a9eb089ecca237603ca2022-12-22T03:36:10ZengSri Lankan Society for MicrobiologySri Lankan Journal of Infectious Diseases2012-81692448-96542014-02-0141222910.4038/sljid.v4i1.60365129To determine the sensitivity and specificity of Polymerase chain reaction (PCR) in the diagnosis of visceral leishmaniasisMS ANJUM0U ANSARI1F HUSSAIN2N KALSOOM3Assistant Consultant of Medicine King Abdullah Medical City, Makkah, Saudi Arabia.M.B.B.S, FCPS (Haematology) Assistant Professor of Pathology (Haematology) Independent Medical College, Faisalabad,M.B.B.S, FCPS (Anaesthesiology) Assistant ProfessorM.B.B.S Medical Officer National Hospital, Faisalabad<p><strong> </strong></p><p><strong>Introduction:</strong> Leishmaniasis is a disease caused by a protozoan parasite of the genus <em>Leishmania,</em> which is transmitted through bites of infected sandflies. It has been reported that the polymerase chain reaction (PCR) is a more sensitive and specific test for the diagnosis of visceral leishmaniasis than bone marrow examination. This recent study is a renewed effort to validate the role of PCR in the diagnosis of visceral leishmaniasis.</p> <p><strong> </strong></p> <p><strong>Objective:</strong> The objective of this study was to determine the sensitivity and specificity of PCR in the diagnosis of visceral leishmaniasis.</p> <p><strong> </strong></p> <p><strong>Study design:</strong> Cross sectional (validation) study carried out in the Haematology department, Armed Forces Institute of Pathology, Rawalpindi. from 25<sup>th</sup> March 2011 to 24<sup>th</sup> March 2012.</p> <p><strong> </strong></p> <p><strong>Subjects and Methods:</strong> A total number of 59 patients with visceral leishmaniasis diagnosed on microscopic bone marrow examination with equal number of negative controls were studied. The subjects were tested for the presence of visceral leishmaniasis by the polymerase chain reaction.</p> <p><strong> </strong></p> <p><strong>Results:</strong> All the 59 patients were found to be positive for visceral leishmaniasis by PCR. None of the negative controls were positive by PCR.</p> <p><strong> </strong></p> <p><strong>Conclusion:</strong> The study validates that PCR is equal to microscopic bone marrow examination in the diagnosis of visceral leishmaniasis.</p><p> </p><p>DOI: <a href="http://dx.doi.org/10.4038/sljid.v4i1.6036">http://dx.doi.org/10.4038/sljid.v4i1.6036</a></p>https://sljid.sljol.info/articles/6036visceral leishmaniasispcrsandfly. |
| spellingShingle | MS ANJUM U ANSARI F HUSSAIN N KALSOOM To determine the sensitivity and specificity of Polymerase chain reaction (PCR) in the diagnosis of visceral leishmaniasis Sri Lankan Journal of Infectious Diseases visceral leishmaniasis pcr sandfly. |
| title | To determine the sensitivity and specificity of Polymerase chain reaction (PCR) in the diagnosis of visceral leishmaniasis |
| title_full | To determine the sensitivity and specificity of Polymerase chain reaction (PCR) in the diagnosis of visceral leishmaniasis |
| title_fullStr | To determine the sensitivity and specificity of Polymerase chain reaction (PCR) in the diagnosis of visceral leishmaniasis |
| title_full_unstemmed | To determine the sensitivity and specificity of Polymerase chain reaction (PCR) in the diagnosis of visceral leishmaniasis |
| title_short | To determine the sensitivity and specificity of Polymerase chain reaction (PCR) in the diagnosis of visceral leishmaniasis |
| title_sort | to determine the sensitivity and specificity of polymerase chain reaction pcr in the diagnosis of visceral leishmaniasis |
| topic | visceral leishmaniasis pcr sandfly. |
| url | https://sljid.sljol.info/articles/6036 |
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