Integration of Metabolomic and Transcriptomic Provides Insights into Anti-Inflammatory Response to <i>trans</i>-10-Hydroxy-2-decenoic Acid on LPS-Stimulated RAW 264.7 Cells

<i>Trans</i>-10-hydroxy-2-decenoic acid (10-HDA) is a unique fatty acid found in royal jelly that possesses potential health benefits such as anti-inflammatory. However, further research is needed to fully understand its mechanisms of action and therapeutic potential for inflammation-ass...

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Main Authors: Minjie Huang, Jie Dong, Xiaodong Tan, Shuyuan Yang, Minghui Xiao, Deqian Wang
Format: Article
Language:English
Published: MDPI AG 2023-08-01
Series:International Journal of Molecular Sciences
Subjects:
Online Access:https://www.mdpi.com/1422-0067/24/16/12666
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author Minjie Huang
Jie Dong
Xiaodong Tan
Shuyuan Yang
Minghui Xiao
Deqian Wang
author_facet Minjie Huang
Jie Dong
Xiaodong Tan
Shuyuan Yang
Minghui Xiao
Deqian Wang
author_sort Minjie Huang
collection DOAJ
description <i>Trans</i>-10-hydroxy-2-decenoic acid (10-HDA) is a unique fatty acid found in royal jelly that possesses potential health benefits such as anti-inflammatory. However, further research is needed to fully understand its mechanisms of action and therapeutic potential for inflammation-associated diseases. In this present study, liquid chromatography–tandem mass spectrometry (LC-MS/MS) and RNA-seq analyses were conducted to comprehensively analyze the in vitro anti-inflammatory effects of 10-HDA on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Our results demonstrated that 128 differentially expressed metabolites and 1721 differentially expressed genes were identified in the 10-HDA-treated groups compared to the LPS groups. Metabolites were significantly enriched in amino acid metabolism pathways, including methionine metabolism, glycine and serine metabolism, and tryptophan metabolism. The differentially expressed genes enrichment analysis indicated that antigen processing and presentation, NOD-like receptor signaling pathway, and arginine biosynthesis were enriched with the administration of 10-had. The correlation analysis revealed that glycerophospholipid metabolism and s-adenosylmethionine-dependent methylation processes might be involved in the response to the 10-HDA treatment. Overall, the findings from this study showed that 10-HDA might involve the modulation of certain signaling pathways involved in the inflammatory response, but further research is needed to determine the safety and efficacy as a therapeutic agent.
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spelling doaj.art-8e97eabbdff74fe88a378a1addcfbd062023-11-19T01:27:00ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-08-0124161266610.3390/ijms241612666Integration of Metabolomic and Transcriptomic Provides Insights into Anti-Inflammatory Response to <i>trans</i>-10-Hydroxy-2-decenoic Acid on LPS-Stimulated RAW 264.7 CellsMinjie Huang0Jie Dong1Xiaodong Tan2Shuyuan Yang3Minghui Xiao4Deqian Wang5Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China<i>Trans</i>-10-hydroxy-2-decenoic acid (10-HDA) is a unique fatty acid found in royal jelly that possesses potential health benefits such as anti-inflammatory. However, further research is needed to fully understand its mechanisms of action and therapeutic potential for inflammation-associated diseases. In this present study, liquid chromatography–tandem mass spectrometry (LC-MS/MS) and RNA-seq analyses were conducted to comprehensively analyze the in vitro anti-inflammatory effects of 10-HDA on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Our results demonstrated that 128 differentially expressed metabolites and 1721 differentially expressed genes were identified in the 10-HDA-treated groups compared to the LPS groups. Metabolites were significantly enriched in amino acid metabolism pathways, including methionine metabolism, glycine and serine metabolism, and tryptophan metabolism. The differentially expressed genes enrichment analysis indicated that antigen processing and presentation, NOD-like receptor signaling pathway, and arginine biosynthesis were enriched with the administration of 10-had. The correlation analysis revealed that glycerophospholipid metabolism and s-adenosylmethionine-dependent methylation processes might be involved in the response to the 10-HDA treatment. Overall, the findings from this study showed that 10-HDA might involve the modulation of certain signaling pathways involved in the inflammatory response, but further research is needed to determine the safety and efficacy as a therapeutic agent.https://www.mdpi.com/1422-0067/24/16/1266610-HDAanti-inflammatoryroyal jellymetabolites
spellingShingle Minjie Huang
Jie Dong
Xiaodong Tan
Shuyuan Yang
Minghui Xiao
Deqian Wang
Integration of Metabolomic and Transcriptomic Provides Insights into Anti-Inflammatory Response to <i>trans</i>-10-Hydroxy-2-decenoic Acid on LPS-Stimulated RAW 264.7 Cells
International Journal of Molecular Sciences
10-HDA
anti-inflammatory
royal jelly
metabolites
title Integration of Metabolomic and Transcriptomic Provides Insights into Anti-Inflammatory Response to <i>trans</i>-10-Hydroxy-2-decenoic Acid on LPS-Stimulated RAW 264.7 Cells
title_full Integration of Metabolomic and Transcriptomic Provides Insights into Anti-Inflammatory Response to <i>trans</i>-10-Hydroxy-2-decenoic Acid on LPS-Stimulated RAW 264.7 Cells
title_fullStr Integration of Metabolomic and Transcriptomic Provides Insights into Anti-Inflammatory Response to <i>trans</i>-10-Hydroxy-2-decenoic Acid on LPS-Stimulated RAW 264.7 Cells
title_full_unstemmed Integration of Metabolomic and Transcriptomic Provides Insights into Anti-Inflammatory Response to <i>trans</i>-10-Hydroxy-2-decenoic Acid on LPS-Stimulated RAW 264.7 Cells
title_short Integration of Metabolomic and Transcriptomic Provides Insights into Anti-Inflammatory Response to <i>trans</i>-10-Hydroxy-2-decenoic Acid on LPS-Stimulated RAW 264.7 Cells
title_sort integration of metabolomic and transcriptomic provides insights into anti inflammatory response to i trans i 10 hydroxy 2 decenoic acid on lps stimulated raw 264 7 cells
topic 10-HDA
anti-inflammatory
royal jelly
metabolites
url https://www.mdpi.com/1422-0067/24/16/12666
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