Integration of Metabolomic and Transcriptomic Provides Insights into Anti-Inflammatory Response to <i>trans</i>-10-Hydroxy-2-decenoic Acid on LPS-Stimulated RAW 264.7 Cells
<i>Trans</i>-10-hydroxy-2-decenoic acid (10-HDA) is a unique fatty acid found in royal jelly that possesses potential health benefits such as anti-inflammatory. However, further research is needed to fully understand its mechanisms of action and therapeutic potential for inflammation-ass...
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MDPI AG
2023-08-01
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author | Minjie Huang Jie Dong Xiaodong Tan Shuyuan Yang Minghui Xiao Deqian Wang |
author_facet | Minjie Huang Jie Dong Xiaodong Tan Shuyuan Yang Minghui Xiao Deqian Wang |
author_sort | Minjie Huang |
collection | DOAJ |
description | <i>Trans</i>-10-hydroxy-2-decenoic acid (10-HDA) is a unique fatty acid found in royal jelly that possesses potential health benefits such as anti-inflammatory. However, further research is needed to fully understand its mechanisms of action and therapeutic potential for inflammation-associated diseases. In this present study, liquid chromatography–tandem mass spectrometry (LC-MS/MS) and RNA-seq analyses were conducted to comprehensively analyze the in vitro anti-inflammatory effects of 10-HDA on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Our results demonstrated that 128 differentially expressed metabolites and 1721 differentially expressed genes were identified in the 10-HDA-treated groups compared to the LPS groups. Metabolites were significantly enriched in amino acid metabolism pathways, including methionine metabolism, glycine and serine metabolism, and tryptophan metabolism. The differentially expressed genes enrichment analysis indicated that antigen processing and presentation, NOD-like receptor signaling pathway, and arginine biosynthesis were enriched with the administration of 10-had. The correlation analysis revealed that glycerophospholipid metabolism and s-adenosylmethionine-dependent methylation processes might be involved in the response to the 10-HDA treatment. Overall, the findings from this study showed that 10-HDA might involve the modulation of certain signaling pathways involved in the inflammatory response, but further research is needed to determine the safety and efficacy as a therapeutic agent. |
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language | English |
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spelling | doaj.art-8e97eabbdff74fe88a378a1addcfbd062023-11-19T01:27:00ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-08-0124161266610.3390/ijms241612666Integration of Metabolomic and Transcriptomic Provides Insights into Anti-Inflammatory Response to <i>trans</i>-10-Hydroxy-2-decenoic Acid on LPS-Stimulated RAW 264.7 CellsMinjie Huang0Jie Dong1Xiaodong Tan2Shuyuan Yang3Minghui Xiao4Deqian Wang5Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China<i>Trans</i>-10-hydroxy-2-decenoic acid (10-HDA) is a unique fatty acid found in royal jelly that possesses potential health benefits such as anti-inflammatory. However, further research is needed to fully understand its mechanisms of action and therapeutic potential for inflammation-associated diseases. In this present study, liquid chromatography–tandem mass spectrometry (LC-MS/MS) and RNA-seq analyses were conducted to comprehensively analyze the in vitro anti-inflammatory effects of 10-HDA on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Our results demonstrated that 128 differentially expressed metabolites and 1721 differentially expressed genes were identified in the 10-HDA-treated groups compared to the LPS groups. Metabolites were significantly enriched in amino acid metabolism pathways, including methionine metabolism, glycine and serine metabolism, and tryptophan metabolism. The differentially expressed genes enrichment analysis indicated that antigen processing and presentation, NOD-like receptor signaling pathway, and arginine biosynthesis were enriched with the administration of 10-had. The correlation analysis revealed that glycerophospholipid metabolism and s-adenosylmethionine-dependent methylation processes might be involved in the response to the 10-HDA treatment. Overall, the findings from this study showed that 10-HDA might involve the modulation of certain signaling pathways involved in the inflammatory response, but further research is needed to determine the safety and efficacy as a therapeutic agent.https://www.mdpi.com/1422-0067/24/16/1266610-HDAanti-inflammatoryroyal jellymetabolites |
spellingShingle | Minjie Huang Jie Dong Xiaodong Tan Shuyuan Yang Minghui Xiao Deqian Wang Integration of Metabolomic and Transcriptomic Provides Insights into Anti-Inflammatory Response to <i>trans</i>-10-Hydroxy-2-decenoic Acid on LPS-Stimulated RAW 264.7 Cells International Journal of Molecular Sciences 10-HDA anti-inflammatory royal jelly metabolites |
title | Integration of Metabolomic and Transcriptomic Provides Insights into Anti-Inflammatory Response to <i>trans</i>-10-Hydroxy-2-decenoic Acid on LPS-Stimulated RAW 264.7 Cells |
title_full | Integration of Metabolomic and Transcriptomic Provides Insights into Anti-Inflammatory Response to <i>trans</i>-10-Hydroxy-2-decenoic Acid on LPS-Stimulated RAW 264.7 Cells |
title_fullStr | Integration of Metabolomic and Transcriptomic Provides Insights into Anti-Inflammatory Response to <i>trans</i>-10-Hydroxy-2-decenoic Acid on LPS-Stimulated RAW 264.7 Cells |
title_full_unstemmed | Integration of Metabolomic and Transcriptomic Provides Insights into Anti-Inflammatory Response to <i>trans</i>-10-Hydroxy-2-decenoic Acid on LPS-Stimulated RAW 264.7 Cells |
title_short | Integration of Metabolomic and Transcriptomic Provides Insights into Anti-Inflammatory Response to <i>trans</i>-10-Hydroxy-2-decenoic Acid on LPS-Stimulated RAW 264.7 Cells |
title_sort | integration of metabolomic and transcriptomic provides insights into anti inflammatory response to i trans i 10 hydroxy 2 decenoic acid on lps stimulated raw 264 7 cells |
topic | 10-HDA anti-inflammatory royal jelly metabolites |
url | https://www.mdpi.com/1422-0067/24/16/12666 |
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