Direct chemical editing of Gram‐positive bacterial cell walls via an enzyme‐catalyzed oxidative coupling reaction
Abstract Chemically manipulating bacterial surface structures, a cutting‐edge research direction in the biomedical field, predominantly relies on metabolic labeling by now. However, this method may involve daunting precursor synthesis and only labels nascent surface structures. Here, we report a fac...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-10-01
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| Series: | Exploration |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/EXP.20220010 |
| _version_ | 1811250349724401664 |
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| author | Hao‐Ran Jia Ya‐Xuan Zhu Yi Liu Yuxin Guo Sayed Mir Sayed Xiao‐Yu Zhu Xiaotong Cheng Fu‐Gen Wu |
| author_facet | Hao‐Ran Jia Ya‐Xuan Zhu Yi Liu Yuxin Guo Sayed Mir Sayed Xiao‐Yu Zhu Xiaotong Cheng Fu‐Gen Wu |
| author_sort | Hao‐Ran Jia |
| collection | DOAJ |
| description | Abstract Chemically manipulating bacterial surface structures, a cutting‐edge research direction in the biomedical field, predominantly relies on metabolic labeling by now. However, this method may involve daunting precursor synthesis and only labels nascent surface structures. Here, we report a facile and rapid modification strategy based on a tyrosinase‐catalyzed oxidative coupling reaction (TyOCR) for bacterial surface engineering. This strategy employs phenol‐tagged small molecules and tyrosinase to initiate direct chemical modification of Gram‐positive bacterial cell walls with high labeling efficiency, while Gram‐negative bacteria are inert to this modification due to the hindrance of an outer membrane. By using the biotin‒avidin system, we further present the selective deposition of various materials, including photosensitizer, magnetic nanoparticle, and horseradish peroxidase, on Gram‐positive bacterial surfaces, and realize the purification/isolation/enrichment and naked‐eye detection of bacterial strains. This work demonstrates that TyOCR is a promising strategy for engineering live bacterial cells. |
| first_indexed | 2024-04-12T16:03:31Z |
| format | Article |
| id | doaj.art-8e9978369d1a44b8aa755e8eff20066a |
| institution | Directory Open Access Journal |
| issn | 2766-8509 2766-2098 |
| language | English |
| last_indexed | 2024-04-12T16:03:31Z |
| publishDate | 2022-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Exploration |
| spelling | doaj.art-8e9978369d1a44b8aa755e8eff20066a2022-12-22T03:26:09ZengWileyExploration2766-85092766-20982022-10-0125n/an/a10.1002/EXP.20220010Direct chemical editing of Gram‐positive bacterial cell walls via an enzyme‐catalyzed oxidative coupling reactionHao‐Ran Jia0Ya‐Xuan Zhu1Yi Liu2Yuxin Guo3Sayed Mir Sayed4Xiao‐Yu Zhu5Xiaotong Cheng6Fu‐Gen Wu7State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering Southeast University Nanjing P. R. ChinaState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering Southeast University Nanjing P. R. ChinaState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering Southeast University Nanjing P. R. ChinaState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering Southeast University Nanjing P. R. ChinaState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering Southeast University Nanjing P. R. ChinaState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering Southeast University Nanjing P. R. ChinaState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering Southeast University Nanjing P. R. ChinaState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering Southeast University Nanjing P. R. ChinaAbstract Chemically manipulating bacterial surface structures, a cutting‐edge research direction in the biomedical field, predominantly relies on metabolic labeling by now. However, this method may involve daunting precursor synthesis and only labels nascent surface structures. Here, we report a facile and rapid modification strategy based on a tyrosinase‐catalyzed oxidative coupling reaction (TyOCR) for bacterial surface engineering. This strategy employs phenol‐tagged small molecules and tyrosinase to initiate direct chemical modification of Gram‐positive bacterial cell walls with high labeling efficiency, while Gram‐negative bacteria are inert to this modification due to the hindrance of an outer membrane. By using the biotin‒avidin system, we further present the selective deposition of various materials, including photosensitizer, magnetic nanoparticle, and horseradish peroxidase, on Gram‐positive bacterial surfaces, and realize the purification/isolation/enrichment and naked‐eye detection of bacterial strains. This work demonstrates that TyOCR is a promising strategy for engineering live bacterial cells.https://doi.org/10.1002/EXP.20220010biosensingcell surface engineeringlive cellsoxidative couplingtyrosinase |
| spellingShingle | Hao‐Ran Jia Ya‐Xuan Zhu Yi Liu Yuxin Guo Sayed Mir Sayed Xiao‐Yu Zhu Xiaotong Cheng Fu‐Gen Wu Direct chemical editing of Gram‐positive bacterial cell walls via an enzyme‐catalyzed oxidative coupling reaction Exploration biosensing cell surface engineering live cells oxidative coupling tyrosinase |
| title | Direct chemical editing of Gram‐positive bacterial cell walls via an enzyme‐catalyzed oxidative coupling reaction |
| title_full | Direct chemical editing of Gram‐positive bacterial cell walls via an enzyme‐catalyzed oxidative coupling reaction |
| title_fullStr | Direct chemical editing of Gram‐positive bacterial cell walls via an enzyme‐catalyzed oxidative coupling reaction |
| title_full_unstemmed | Direct chemical editing of Gram‐positive bacterial cell walls via an enzyme‐catalyzed oxidative coupling reaction |
| title_short | Direct chemical editing of Gram‐positive bacterial cell walls via an enzyme‐catalyzed oxidative coupling reaction |
| title_sort | direct chemical editing of gram positive bacterial cell walls via an enzyme catalyzed oxidative coupling reaction |
| topic | biosensing cell surface engineering live cells oxidative coupling tyrosinase |
| url | https://doi.org/10.1002/EXP.20220010 |
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