Significance of circulating microRNAs in diabetes mellitus type 2 and platelet reactivity: bioinformatic analysis and review

Abstract In the light of growing global epidemic of type 2 diabetes mellitus (T2DM), significant efforts are made to discover next-generation biomarkers for early detection of the disease. Multiple mechanisms including inflammatory response, abnormal insulin secretion and glucose metabolism contribu...

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Main Authors: Justyna Pordzik, Daniel Jakubik, Joanna Jarosz-Popek, Zofia Wicik, Ceren Eyileten, Salvatore De Rosa, Ciro Indolfi, Jolanta M. Siller-Matula, Pamela Czajka, Marek Postula
Format: Article
Language:English
Published: BMC 2019-08-01
Series:Cardiovascular Diabetology
Subjects:
Online Access:http://link.springer.com/article/10.1186/s12933-019-0918-x
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author Justyna Pordzik
Daniel Jakubik
Joanna Jarosz-Popek
Zofia Wicik
Ceren Eyileten
Salvatore De Rosa
Ciro Indolfi
Jolanta M. Siller-Matula
Pamela Czajka
Marek Postula
author_facet Justyna Pordzik
Daniel Jakubik
Joanna Jarosz-Popek
Zofia Wicik
Ceren Eyileten
Salvatore De Rosa
Ciro Indolfi
Jolanta M. Siller-Matula
Pamela Czajka
Marek Postula
author_sort Justyna Pordzik
collection DOAJ
description Abstract In the light of growing global epidemic of type 2 diabetes mellitus (T2DM), significant efforts are made to discover next-generation biomarkers for early detection of the disease. Multiple mechanisms including inflammatory response, abnormal insulin secretion and glucose metabolism contribute to the development of T2DM. Platelet activation, on the other hand, is known to be one of the underlying mechanisms of atherosclerosis, which is a common T2DM complication that frequently results in ischemic events at later stages of the disease. Available data suggest that platelets contain large amounts of microRNAs (miRNAs) that are found in circulating body fluids, including the blood. Since miRNAs have been illustrated to play an important role in metabolic homeostasis through regulation of multiple genes, they attracted substantial scientific interest as diagnostic and prognostic biomarkers in T2DM. Various miRNAs, as well as their target genes are implicated in the complex pathophysiology of T2DM. This article will first review the different miRNAs studied in the context of T2DM and platelet reactivity, and subsequently present original results from bioinformatic analyses of published reports, identifying a common gene (PRKAR1A) linked to glucose metabolism, blood coagulation and insulin signalling and targeted by miRNAs in T2DM. Moreover, miRNA–target gene interaction networks built upon Gene Ontology information from electronic databases were developed. According to our results, miR-30a-5p, miR-30d-5p and miR-30c-5p are the most widely regulated miRNAs across all specified ontologies, hence they are the most promising biomarkers of T2DM to be investigated in future clinical studies.
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spelling doaj.art-8e9cc054277849699a96ac40b9b31fa42022-12-21T19:21:22ZengBMCCardiovascular Diabetology1475-28402019-08-0118111910.1186/s12933-019-0918-xSignificance of circulating microRNAs in diabetes mellitus type 2 and platelet reactivity: bioinformatic analysis and reviewJustyna Pordzik0Daniel Jakubik1Joanna Jarosz-Popek2Zofia Wicik3Ceren Eyileten4Salvatore De Rosa5Ciro Indolfi6Jolanta M. Siller-Matula7Pamela Czajka8Marek Postula9Department of Experimental and Clinical Pharmacology, Center for Preclinical Research and Technology CEPT, Medical University of WarsawDepartment of Experimental and Clinical Pharmacology, Center for Preclinical Research and Technology CEPT, Medical University of WarsawDepartment of Experimental and Clinical Pharmacology, Center for Preclinical Research and Technology CEPT, Medical University of WarsawRheumatology Division, Hospital das Clinicas HCFMUSP, University of São Paulo, School of MedicineDepartment of Experimental and Clinical Pharmacology, Center for Preclinical Research and Technology CEPT, Medical University of WarsawDivision of Cardiology, Department of Medical and Surgical Sciences, “Magna Graecia” UniversityDivision of Cardiology, Department of Medical and Surgical Sciences, “Magna Graecia” UniversityDepartment of Cardiology, Medical University of ViennaDepartment of Experimental and Clinical Pharmacology, Center for Preclinical Research and Technology CEPT, Medical University of WarsawDepartment of Experimental and Clinical Pharmacology, Center for Preclinical Research and Technology CEPT, Medical University of WarsawAbstract In the light of growing global epidemic of type 2 diabetes mellitus (T2DM), significant efforts are made to discover next-generation biomarkers for early detection of the disease. Multiple mechanisms including inflammatory response, abnormal insulin secretion and glucose metabolism contribute to the development of T2DM. Platelet activation, on the other hand, is known to be one of the underlying mechanisms of atherosclerosis, which is a common T2DM complication that frequently results in ischemic events at later stages of the disease. Available data suggest that platelets contain large amounts of microRNAs (miRNAs) that are found in circulating body fluids, including the blood. Since miRNAs have been illustrated to play an important role in metabolic homeostasis through regulation of multiple genes, they attracted substantial scientific interest as diagnostic and prognostic biomarkers in T2DM. Various miRNAs, as well as their target genes are implicated in the complex pathophysiology of T2DM. This article will first review the different miRNAs studied in the context of T2DM and platelet reactivity, and subsequently present original results from bioinformatic analyses of published reports, identifying a common gene (PRKAR1A) linked to glucose metabolism, blood coagulation and insulin signalling and targeted by miRNAs in T2DM. Moreover, miRNA–target gene interaction networks built upon Gene Ontology information from electronic databases were developed. According to our results, miR-30a-5p, miR-30d-5p and miR-30c-5p are the most widely regulated miRNAs across all specified ontologies, hence they are the most promising biomarkers of T2DM to be investigated in future clinical studies.http://link.springer.com/article/10.1186/s12933-019-0918-xMicroRNADiabetes mellitus type 2Platelet reactivityBiomarkerBioinformatic analysismiRNA–gene target interaction
spellingShingle Justyna Pordzik
Daniel Jakubik
Joanna Jarosz-Popek
Zofia Wicik
Ceren Eyileten
Salvatore De Rosa
Ciro Indolfi
Jolanta M. Siller-Matula
Pamela Czajka
Marek Postula
Significance of circulating microRNAs in diabetes mellitus type 2 and platelet reactivity: bioinformatic analysis and review
Cardiovascular Diabetology
MicroRNA
Diabetes mellitus type 2
Platelet reactivity
Biomarker
Bioinformatic analysis
miRNA–gene target interaction
title Significance of circulating microRNAs in diabetes mellitus type 2 and platelet reactivity: bioinformatic analysis and review
title_full Significance of circulating microRNAs in diabetes mellitus type 2 and platelet reactivity: bioinformatic analysis and review
title_fullStr Significance of circulating microRNAs in diabetes mellitus type 2 and platelet reactivity: bioinformatic analysis and review
title_full_unstemmed Significance of circulating microRNAs in diabetes mellitus type 2 and platelet reactivity: bioinformatic analysis and review
title_short Significance of circulating microRNAs in diabetes mellitus type 2 and platelet reactivity: bioinformatic analysis and review
title_sort significance of circulating micrornas in diabetes mellitus type 2 and platelet reactivity bioinformatic analysis and review
topic MicroRNA
Diabetes mellitus type 2
Platelet reactivity
Biomarker
Bioinformatic analysis
miRNA–gene target interaction
url http://link.springer.com/article/10.1186/s12933-019-0918-x
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