Pharmacological Investigation of Cav for its Potential Against Thrombosis in Experimental Models
Background Thrombosis is a major disorder which is an outcome of an imbalance in the hemostatic system that develop undesirable blood clot and hinder blood circulation. Purpose The current study was designed to verify the potential of aqueous methanolic crude extract of Asphodelus tenuifolius Cav ....
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2022-09-01
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| Series: | Dose-Response |
| Online Access: | https://doi.org/10.1177/15593258221127566 |
| _version_ | 1797995186668699648 |
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| author | Humaira Gul Ayesha Jamshed Qaiser Jabeen |
| author_facet | Humaira Gul Ayesha Jamshed Qaiser Jabeen |
| author_sort | Humaira Gul |
| collection | DOAJ |
| description | Background Thrombosis is a major disorder which is an outcome of an imbalance in the hemostatic system that develop undesirable blood clot and hinder blood circulation. Purpose The current study was designed to verify the potential of aqueous methanolic crude extract of Asphodelus tenuifolius Cav . (At.Cr), used traditionally as remedy in circulatory problems. Research Design Antioxidant activity, FTIR, and HPLC analysis were performed. In-vitro clot lysis assay was performed on human blood samples, and in-vivo acute pulmonary thromboembolism model was developed by administering the mixture of collagen and epinephrine in tail vein of mice. Carrageenan-induced thrombosis and FeCl 3 -induced carotid arterial thrombosis models were developed in rats. Results At.Cr demonstrated significant increase in lysis of human blood clot. Bleeding and clotting times were increased dose-dependently. Lungs histology showed clear alveolar spaces with decreased red blood cells congestion. Reduction in infarcted tail length, augmentation in prothrombin time, and activated partial thromboplastin time with decrease in platelet count were observed. At.Cr also prolonged the arterial occlusion time and reduced the weight of thrombus and TXB 2 levels dose-dependently. Conclusions The results demonstrated the antithrombotic and thrombolytic potential of At.Cr due to activation of coagulation factors through extrinsic and intrinsic pathways. |
| first_indexed | 2024-04-11T09:57:25Z |
| format | Article |
| id | doaj.art-8e9ce262ecc641a0969dc5d01c4d017f |
| institution | Directory Open Access Journal |
| issn | 1559-3258 |
| language | English |
| last_indexed | 2024-04-11T09:57:25Z |
| publishDate | 2022-09-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Dose-Response |
| spelling | doaj.art-8e9ce262ecc641a0969dc5d01c4d017f2022-12-22T04:30:31ZengSAGE PublishingDose-Response1559-32582022-09-012010.1177/15593258221127566Pharmacological Investigation of Cav for its Potential Against Thrombosis in Experimental ModelsHumaira GulAyesha JamshedQaiser JabeenBackground Thrombosis is a major disorder which is an outcome of an imbalance in the hemostatic system that develop undesirable blood clot and hinder blood circulation. Purpose The current study was designed to verify the potential of aqueous methanolic crude extract of Asphodelus tenuifolius Cav . (At.Cr), used traditionally as remedy in circulatory problems. Research Design Antioxidant activity, FTIR, and HPLC analysis were performed. In-vitro clot lysis assay was performed on human blood samples, and in-vivo acute pulmonary thromboembolism model was developed by administering the mixture of collagen and epinephrine in tail vein of mice. Carrageenan-induced thrombosis and FeCl 3 -induced carotid arterial thrombosis models were developed in rats. Results At.Cr demonstrated significant increase in lysis of human blood clot. Bleeding and clotting times were increased dose-dependently. Lungs histology showed clear alveolar spaces with decreased red blood cells congestion. Reduction in infarcted tail length, augmentation in prothrombin time, and activated partial thromboplastin time with decrease in platelet count were observed. At.Cr also prolonged the arterial occlusion time and reduced the weight of thrombus and TXB 2 levels dose-dependently. Conclusions The results demonstrated the antithrombotic and thrombolytic potential of At.Cr due to activation of coagulation factors through extrinsic and intrinsic pathways.https://doi.org/10.1177/15593258221127566 |
| spellingShingle | Humaira Gul Ayesha Jamshed Qaiser Jabeen Pharmacological Investigation of Cav for its Potential Against Thrombosis in Experimental Models Dose-Response |
| title | Pharmacological Investigation of Cav for its Potential Against Thrombosis in Experimental Models |
| title_full | Pharmacological Investigation of Cav for its Potential Against Thrombosis in Experimental Models |
| title_fullStr | Pharmacological Investigation of Cav for its Potential Against Thrombosis in Experimental Models |
| title_full_unstemmed | Pharmacological Investigation of Cav for its Potential Against Thrombosis in Experimental Models |
| title_short | Pharmacological Investigation of Cav for its Potential Against Thrombosis in Experimental Models |
| title_sort | pharmacological investigation of cav for its potential against thrombosis in experimental models |
| url | https://doi.org/10.1177/15593258221127566 |
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