CCL18 Expression Is Higher in a Glioblastoma Multiforme Tumor than in the Peritumoral Area and Causes the Migration of Tumor Cells Sensitized by Hypoxia
Glioblastoma multiforme (GBM) is a brain tumor with a very poor prognosis. For this reason, researchers worldwide study the impact of the tumor microenvironment in GBM, such as the effect of chemokines. In the present study, we focus on the role of the chemokine CCL18 and its receptors in the GBM tu...
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MDPI AG
2022-08-01
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| Series: | International Journal of Molecular Sciences |
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| author | Szymon Grochans Jan Korbecki Donata Simińska Wojciech Żwierełło Sylwia Rzeszotek Agnieszka Kolasa Klaudyna Kojder Maciej Tarnowski Dariusz Chlubek Irena Baranowska-Bosiacka |
| author_facet | Szymon Grochans Jan Korbecki Donata Simińska Wojciech Żwierełło Sylwia Rzeszotek Agnieszka Kolasa Klaudyna Kojder Maciej Tarnowski Dariusz Chlubek Irena Baranowska-Bosiacka |
| author_sort | Szymon Grochans |
| collection | DOAJ |
| description | Glioblastoma multiforme (GBM) is a brain tumor with a very poor prognosis. For this reason, researchers worldwide study the impact of the tumor microenvironment in GBM, such as the effect of chemokines. In the present study, we focus on the role of the chemokine CCL18 and its receptors in the GBM tumor. We measured the expression of CCL18, CCR8 and PITPNM3 in the GMB tumor from patients (16 men and 12 women) using quantitative real-time polymerase chain reaction. To investigate the effect of CCL18 on the proliferation and migration of GBM cells, experiments were performed using U-87 MG cells. The results showed that CCL18 expression was higher in the GBM tumor than in the peritumoral area. The women had a decreased expression of PITPNM3 receptor in the GBM tumor, while in the men a lower expression of CCR8 was observed. The hypoxia-mimetic agent, cobalt chloride (CoCl<sub>2</sub>), increased the expression of CCL18 and PITPNM3 and thereby sensitized U-87 MG cells to CCL18, which did not affect the proliferation of U-87 MG cells but increased the migration of the test cells. The results indicate that GBM cells migrate from hypoxic areas, which may be important in understanding the mechanisms of tumorigenesis. |
| first_indexed | 2024-03-09T05:21:21Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T05:21:21Z |
| publishDate | 2022-08-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-8e9ddf2b5e504bacb6adab64a0dcd9e22023-12-03T12:40:29ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-08-012315853610.3390/ijms23158536CCL18 Expression Is Higher in a Glioblastoma Multiforme Tumor than in the Peritumoral Area and Causes the Migration of Tumor Cells Sensitized by HypoxiaSzymon Grochans0Jan Korbecki1Donata Simińska2Wojciech Żwierełło3Sylwia Rzeszotek4Agnieszka Kolasa5Klaudyna Kojder6Maciej Tarnowski7Dariusz Chlubek8Irena Baranowska-Bosiacka9Department of Biochemistry and Medical Chemistry, Pomeranian Medical University in Szczecin, Powstańców Wlkp. 72, 70-111 Szczecin, PolandDepartment of Biochemistry and Medical Chemistry, Pomeranian Medical University in Szczecin, Powstańców Wlkp. 72, 70-111 Szczecin, PolandDepartment of Biochemistry and Medical Chemistry, Pomeranian Medical University in Szczecin, Powstańców Wlkp. 72, 70-111 Szczecin, PolandDepartment of Medical Chemistry, Pomeranian Medical University in Szczecin, Powstańców Wlkp. 72, 70-111 Szczecin, PolandDepartment of Embryology and Histology, Pomeranian Medical University in Szczecin, Powstańców Wlkp. 72, 70-111 Szczecin, PolandDepartment of Embryology and Histology, Pomeranian Medical University in Szczecin, Powstańców Wlkp. 72, 70-111 Szczecin, PolandDepartment of Anaesthesiology and Intensive Care, Pomeranian Medical University in Szczecin, Unii 13 Lubelskiej 1, 71-281 Szczecin, PolandDepartment of Physiology, Pomeranian Medical University in Szczecin, Powstańców Wlkp. 72, 70-111 Szczecin, PolandDepartment of Biochemistry and Medical Chemistry, Pomeranian Medical University in Szczecin, Powstańców Wlkp. 72, 70-111 Szczecin, PolandDepartment of Biochemistry and Medical Chemistry, Pomeranian Medical University in Szczecin, Powstańców Wlkp. 72, 70-111 Szczecin, PolandGlioblastoma multiforme (GBM) is a brain tumor with a very poor prognosis. For this reason, researchers worldwide study the impact of the tumor microenvironment in GBM, such as the effect of chemokines. In the present study, we focus on the role of the chemokine CCL18 and its receptors in the GBM tumor. We measured the expression of CCL18, CCR8 and PITPNM3 in the GMB tumor from patients (16 men and 12 women) using quantitative real-time polymerase chain reaction. To investigate the effect of CCL18 on the proliferation and migration of GBM cells, experiments were performed using U-87 MG cells. The results showed that CCL18 expression was higher in the GBM tumor than in the peritumoral area. The women had a decreased expression of PITPNM3 receptor in the GBM tumor, while in the men a lower expression of CCR8 was observed. The hypoxia-mimetic agent, cobalt chloride (CoCl<sub>2</sub>), increased the expression of CCL18 and PITPNM3 and thereby sensitized U-87 MG cells to CCL18, which did not affect the proliferation of U-87 MG cells but increased the migration of the test cells. The results indicate that GBM cells migrate from hypoxic areas, which may be important in understanding the mechanisms of tumorigenesis.https://www.mdpi.com/1422-0067/23/15/8536CCL18PITPN3CCR8hypoxiaglioblastoma multiformetumor-associated macrophage |
| spellingShingle | Szymon Grochans Jan Korbecki Donata Simińska Wojciech Żwierełło Sylwia Rzeszotek Agnieszka Kolasa Klaudyna Kojder Maciej Tarnowski Dariusz Chlubek Irena Baranowska-Bosiacka CCL18 Expression Is Higher in a Glioblastoma Multiforme Tumor than in the Peritumoral Area and Causes the Migration of Tumor Cells Sensitized by Hypoxia International Journal of Molecular Sciences CCL18 PITPN3 CCR8 hypoxia glioblastoma multiforme tumor-associated macrophage |
| title | CCL18 Expression Is Higher in a Glioblastoma Multiforme Tumor than in the Peritumoral Area and Causes the Migration of Tumor Cells Sensitized by Hypoxia |
| title_full | CCL18 Expression Is Higher in a Glioblastoma Multiforme Tumor than in the Peritumoral Area and Causes the Migration of Tumor Cells Sensitized by Hypoxia |
| title_fullStr | CCL18 Expression Is Higher in a Glioblastoma Multiforme Tumor than in the Peritumoral Area and Causes the Migration of Tumor Cells Sensitized by Hypoxia |
| title_full_unstemmed | CCL18 Expression Is Higher in a Glioblastoma Multiforme Tumor than in the Peritumoral Area and Causes the Migration of Tumor Cells Sensitized by Hypoxia |
| title_short | CCL18 Expression Is Higher in a Glioblastoma Multiforme Tumor than in the Peritumoral Area and Causes the Migration of Tumor Cells Sensitized by Hypoxia |
| title_sort | ccl18 expression is higher in a glioblastoma multiforme tumor than in the peritumoral area and causes the migration of tumor cells sensitized by hypoxia |
| topic | CCL18 PITPN3 CCR8 hypoxia glioblastoma multiforme tumor-associated macrophage |
| url | https://www.mdpi.com/1422-0067/23/15/8536 |
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