Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts <it>Torilis leptophylla</it> L
<p>Abstract</p> <p>Background</p> <p>The aim of this study was to screen various solvent extracts of whole plant of <it>Torilis leptophylla</it> to display potent antioxidant activity <it>in vitro</it> and <it>in vivo</it>, total phen...
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| Format: | Article |
| Language: | English |
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BMC
2012-11-01
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| Series: | BMC Complementary and Alternative Medicine |
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| Online Access: | http://www.biomedcentral.com/1472-6882/12/221 |
| _version_ | 1818211557543247872 |
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| author | Saeed Naima Khan Muhammad R Shabbir Maria |
| author_facet | Saeed Naima Khan Muhammad R Shabbir Maria |
| author_sort | Saeed Naima |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The aim of this study was to screen various solvent extracts of whole plant of <it>Torilis leptophylla</it> to display potent antioxidant activity <it>in vitro</it> and <it>in vivo</it>, total phenolic and flavonoid contents in order to find possible sources for future novel antioxidants in food and pharmaceutical formulations.</p> <p>Material and methods</p> <p>A detailed study was performed on the antioxidant activity of the methanol extract of whole plant of <it>Torilis leptophylla</it> (TLM) and its derived fractions {<it>n</it>-hexane (TLH), chloroform (TLC) ethyl acetate (TLE) <it>n</it>-butanol (TLB) and residual aqueous fraction (TLA)} by <it>in vitro</it> chemical analyses and carbon tetrachloride (CCl<sub>4</sub>) induced hepatic injuries (lipid peroxidation and glutathione contents) in male Sprague-Dawley rat. The total yield, total phenolic (TPC) and total flavonoid contents (TFC) of all the fractions were also determined. TLM was also subjected to preliminary phytochemical screening test for various constituents.</p> <p>Results</p> <p>The total phenolic contents (TPC) (121.9±3.1 mg GAE/g extract) of TLM while total flavonoid contents (TFC) of TLE (60.9 ±2.2 mg RTE/g extract) were found significantly higher as compared to other solvent fractions. Phytochemical screening of TLM revealed the presence of alkaloids, anthraquinones, cardiac glycosides, coumarins, flavonoids, saponins, phlobatannins, tannins and terpenoids. The EC<sub>50</sub> values based on the DPPH (41.0±1 μg/ml), ABTS (10.0±0.9 μg/ml) and phosphomolybdate (10.7±2 μg/ml) for TLB, hydroxyl radicals (8.0±1 μg/ml) for TLC, superoxide radicals (57.0±0.3 μg/ml) for TLM and hydrogen peroxide radicals (68.0±2 μg/ml) for TLE were generally lower showing potential antioxidant properties. A significant but marginal positive correlation was found between TPC and EC<sub>50</sub> values for DPPH, hydroxyl, phosphomolybdate and ABTS, whereas another weak and positive correlation was determined between TFC and EC<sub>50</sub> values for superoxide anion and hydroxyl radicals. Results of <it>in vivo</it> experiment revealed that administration of CCl<sub>4</sub> caused a significant increase in lipid peroxidation (TBARS) while decrease in GSH contents of liver. In contrast, TLM (200 mg/kg bw) and silymarin (50 mg/kg bw) co-treatment effectively prevented these alterations and maintained the antioxidant status.</p> <p>Conclusion</p> <p>Data from present results revealed that <it>Torilis leptophylla</it> act as an antioxidant agent due to its free radical scavenging and cytoprotective activity.</p> |
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| spelling | doaj.art-8e9ef2b804e749ab8500239105762bbe2022-12-22T00:36:13ZengBMCBMC Complementary and Alternative Medicine1472-68822012-11-0112122110.1186/1472-6882-12-221Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts <it>Torilis leptophylla</it> LSaeed NaimaKhan Muhammad RShabbir Maria<p>Abstract</p> <p>Background</p> <p>The aim of this study was to screen various solvent extracts of whole plant of <it>Torilis leptophylla</it> to display potent antioxidant activity <it>in vitro</it> and <it>in vivo</it>, total phenolic and flavonoid contents in order to find possible sources for future novel antioxidants in food and pharmaceutical formulations.</p> <p>Material and methods</p> <p>A detailed study was performed on the antioxidant activity of the methanol extract of whole plant of <it>Torilis leptophylla</it> (TLM) and its derived fractions {<it>n</it>-hexane (TLH), chloroform (TLC) ethyl acetate (TLE) <it>n</it>-butanol (TLB) and residual aqueous fraction (TLA)} by <it>in vitro</it> chemical analyses and carbon tetrachloride (CCl<sub>4</sub>) induced hepatic injuries (lipid peroxidation and glutathione contents) in male Sprague-Dawley rat. The total yield, total phenolic (TPC) and total flavonoid contents (TFC) of all the fractions were also determined. TLM was also subjected to preliminary phytochemical screening test for various constituents.</p> <p>Results</p> <p>The total phenolic contents (TPC) (121.9±3.1 mg GAE/g extract) of TLM while total flavonoid contents (TFC) of TLE (60.9 ±2.2 mg RTE/g extract) were found significantly higher as compared to other solvent fractions. Phytochemical screening of TLM revealed the presence of alkaloids, anthraquinones, cardiac glycosides, coumarins, flavonoids, saponins, phlobatannins, tannins and terpenoids. The EC<sub>50</sub> values based on the DPPH (41.0±1 μg/ml), ABTS (10.0±0.9 μg/ml) and phosphomolybdate (10.7±2 μg/ml) for TLB, hydroxyl radicals (8.0±1 μg/ml) for TLC, superoxide radicals (57.0±0.3 μg/ml) for TLM and hydrogen peroxide radicals (68.0±2 μg/ml) for TLE were generally lower showing potential antioxidant properties. A significant but marginal positive correlation was found between TPC and EC<sub>50</sub> values for DPPH, hydroxyl, phosphomolybdate and ABTS, whereas another weak and positive correlation was determined between TFC and EC<sub>50</sub> values for superoxide anion and hydroxyl radicals. Results of <it>in vivo</it> experiment revealed that administration of CCl<sub>4</sub> caused a significant increase in lipid peroxidation (TBARS) while decrease in GSH contents of liver. In contrast, TLM (200 mg/kg bw) and silymarin (50 mg/kg bw) co-treatment effectively prevented these alterations and maintained the antioxidant status.</p> <p>Conclusion</p> <p>Data from present results revealed that <it>Torilis leptophylla</it> act as an antioxidant agent due to its free radical scavenging and cytoprotective activity.</p>http://www.biomedcentral.com/1472-6882/12/221<it>Torilis leptophylla</it>DPPHAntioxidantOxidative stressTBARS |
| spellingShingle | Saeed Naima Khan Muhammad R Shabbir Maria Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts <it>Torilis leptophylla</it> L BMC Complementary and Alternative Medicine <it>Torilis leptophylla</it> DPPH Antioxidant Oxidative stress TBARS |
| title | Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts <it>Torilis leptophylla</it> L |
| title_full | Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts <it>Torilis leptophylla</it> L |
| title_fullStr | Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts <it>Torilis leptophylla</it> L |
| title_full_unstemmed | Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts <it>Torilis leptophylla</it> L |
| title_short | Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts <it>Torilis leptophylla</it> L |
| title_sort | antioxidant activity total phenolic and total flavonoid contents of whole plant extracts it torilis leptophylla it l |
| topic | <it>Torilis leptophylla</it> DPPH Antioxidant Oxidative stress TBARS |
| url | http://www.biomedcentral.com/1472-6882/12/221 |
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