Analyses of N-Terminal pro-brain natriuretic peptide, cardiac troponin T, and creatine kinase MB in pericardial fluid in sudden cardiac death caused by ischemic heart disease
Background: Biochemical analyses of N-terminal pro-brain natriuretic peptide (NT-proBNP), cardiac troponin T (cTnT), and creatine kinase MB (CK-MB) have been reported to be valuable for the auxiliary diagnosis of sudden cardiac death (SCD) in previous forensic studies. Aims and Objectives: The prese...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | Journal of Forensic Science and Medicine |
| Subjects: | |
| Online Access: | http://www.jfsmonline.com/article.asp?issn=2349-5014;year=2022;volume=8;issue=4;spage=135;epage=141;aulast=Cao |
| _version_ | 1797954873317130240 |
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| author | Zhipeng Cao Tianqi Wang Shao-Huang Wu Zihan Liao Baoli Zhu Rui Zhao |
| author_facet | Zhipeng Cao Tianqi Wang Shao-Huang Wu Zihan Liao Baoli Zhu Rui Zhao |
| author_sort | Zhipeng Cao |
| collection | DOAJ |
| description | Background: Biochemical analyses of N-terminal pro-brain natriuretic peptide (NT-proBNP), cardiac troponin T (cTnT), and creatine kinase MB (CK-MB) have been reported to be valuable for the auxiliary diagnosis of sudden cardiac death (SCD) in previous forensic studies. Aims and Objectives: The present study aimed to evaluate the diagnostic efficiency of combined analyses of NT-proBNP, cTnT and CK-MB in the pericardial fluid for forensic diagnosis of SCD caused by ischemic heart disease. Materials and Methods: Levels of NT-proBNP, cTnT, and CK-MB in the pericardial fluid of 132 medicolegal autopsy cases were obtained through electrochemiluminescence method. Results: NT-proBNP, cTnT, and CK-MB levels were significantly elevated in SCD cases (P < 0.05). Receiver-operating characteristics (ROC) analysis showed that NT-proBNP, cTnT, and CK-MB have diagnostic value for the diagnosis of SCD: NT-proBNP, cutoff value of 2236 pg/ml; cTnT, cutoff value of 199.51 ng/ml; CK-MB: cutoff value of 2742.5 ng/ml, and the combined analyses of these three biomarkers have better diagnostic efficiency than each single biomarker alone. Moreover, the causes of SCD were sub-divided into acute ischemic heart disease, acute myocardial infarction (AMI), and recurrent myocardial infarction subgroups for further analysis, which revealed that the ratio of cTnT/CK-MB could be used to distinguish AMI with the cutoff value of 0.1085 estimated by ROC analysis. Conclusion: These observations suggested that the postmortem biochemical analyses of NT-proBNP, cTnT, and CK-MB in the pericardial fluid may assist to diagnose SCD in forensic practice, and the combined analyses of multiple biomarkers have better diagnostic efficiency than each single biomarker alone. On the basis of the postmortem biochemical analyses of NT-proBNP, cTnT and CK-MB, combining the ratio of cTnT/CK-MB could be used to distinguish AMI. |
| first_indexed | 2024-04-10T23:24:18Z |
| format | Article |
| id | doaj.art-8e9f05ec4aeb423cb19aa4d8af5ed2f4 |
| institution | Directory Open Access Journal |
| issn | 2349-5014 |
| language | English |
| last_indexed | 2024-04-10T23:24:18Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Forensic Science and Medicine |
| spelling | doaj.art-8e9f05ec4aeb423cb19aa4d8af5ed2f42023-01-12T12:42:34ZengWolters Kluwer Medknow PublicationsJournal of Forensic Science and Medicine2349-50142022-01-018413514110.4103/jfsm.jfsm_124_22Analyses of N-Terminal pro-brain natriuretic peptide, cardiac troponin T, and creatine kinase MB in pericardial fluid in sudden cardiac death caused by ischemic heart diseaseZhipeng CaoTianqi WangShao-Huang WuZihan LiaoBaoli ZhuRui ZhaoBackground: Biochemical analyses of N-terminal pro-brain natriuretic peptide (NT-proBNP), cardiac troponin T (cTnT), and creatine kinase MB (CK-MB) have been reported to be valuable for the auxiliary diagnosis of sudden cardiac death (SCD) in previous forensic studies. Aims and Objectives: The present study aimed to evaluate the diagnostic efficiency of combined analyses of NT-proBNP, cTnT and CK-MB in the pericardial fluid for forensic diagnosis of SCD caused by ischemic heart disease. Materials and Methods: Levels of NT-proBNP, cTnT, and CK-MB in the pericardial fluid of 132 medicolegal autopsy cases were obtained through electrochemiluminescence method. Results: NT-proBNP, cTnT, and CK-MB levels were significantly elevated in SCD cases (P < 0.05). Receiver-operating characteristics (ROC) analysis showed that NT-proBNP, cTnT, and CK-MB have diagnostic value for the diagnosis of SCD: NT-proBNP, cutoff value of 2236 pg/ml; cTnT, cutoff value of 199.51 ng/ml; CK-MB: cutoff value of 2742.5 ng/ml, and the combined analyses of these three biomarkers have better diagnostic efficiency than each single biomarker alone. Moreover, the causes of SCD were sub-divided into acute ischemic heart disease, acute myocardial infarction (AMI), and recurrent myocardial infarction subgroups for further analysis, which revealed that the ratio of cTnT/CK-MB could be used to distinguish AMI with the cutoff value of 0.1085 estimated by ROC analysis. Conclusion: These observations suggested that the postmortem biochemical analyses of NT-proBNP, cTnT, and CK-MB in the pericardial fluid may assist to diagnose SCD in forensic practice, and the combined analyses of multiple biomarkers have better diagnostic efficiency than each single biomarker alone. On the basis of the postmortem biochemical analyses of NT-proBNP, cTnT and CK-MB, combining the ratio of cTnT/CK-MB could be used to distinguish AMI.http://www.jfsmonline.com/article.asp?issn=2349-5014;year=2022;volume=8;issue=4;spage=135;epage=141;aulast=Caocardiac troponin tcreatine kinase mbischemic heart diseasen-terminal pro-brain natriuretic peptidepericardial fluidpostmortem biochemistrysudden cardiac death |
| spellingShingle | Zhipeng Cao Tianqi Wang Shao-Huang Wu Zihan Liao Baoli Zhu Rui Zhao Analyses of N-Terminal pro-brain natriuretic peptide, cardiac troponin T, and creatine kinase MB in pericardial fluid in sudden cardiac death caused by ischemic heart disease Journal of Forensic Science and Medicine cardiac troponin t creatine kinase mb ischemic heart disease n-terminal pro-brain natriuretic peptide pericardial fluid postmortem biochemistry sudden cardiac death |
| title | Analyses of N-Terminal pro-brain natriuretic peptide, cardiac troponin T, and creatine kinase MB in pericardial fluid in sudden cardiac death caused by ischemic heart disease |
| title_full | Analyses of N-Terminal pro-brain natriuretic peptide, cardiac troponin T, and creatine kinase MB in pericardial fluid in sudden cardiac death caused by ischemic heart disease |
| title_fullStr | Analyses of N-Terminal pro-brain natriuretic peptide, cardiac troponin T, and creatine kinase MB in pericardial fluid in sudden cardiac death caused by ischemic heart disease |
| title_full_unstemmed | Analyses of N-Terminal pro-brain natriuretic peptide, cardiac troponin T, and creatine kinase MB in pericardial fluid in sudden cardiac death caused by ischemic heart disease |
| title_short | Analyses of N-Terminal pro-brain natriuretic peptide, cardiac troponin T, and creatine kinase MB in pericardial fluid in sudden cardiac death caused by ischemic heart disease |
| title_sort | analyses of n terminal pro brain natriuretic peptide cardiac troponin t and creatine kinase mb in pericardial fluid in sudden cardiac death caused by ischemic heart disease |
| topic | cardiac troponin t creatine kinase mb ischemic heart disease n-terminal pro-brain natriuretic peptide pericardial fluid postmortem biochemistry sudden cardiac death |
| url | http://www.jfsmonline.com/article.asp?issn=2349-5014;year=2022;volume=8;issue=4;spage=135;epage=141;aulast=Cao |
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