Peculiarities of glucose and glycerol metabolism in Nocardia vaccinii IMB B-7405
It has been established that in cells of Nocardia vaccinii IMB B-7405 (surfactant producer) glucose catabolism is performed through pentose phosphate cycle as well as through gluconate (activity of NAD+-dependent glucose-6- phosphate dehydrogenase and FAD+-dependent glucose dehydrogenase 835 ± 41 a...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2015-04-01
|
| Series: | The Ukrainian Biochemical Journal |
| Subjects: | |
| Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2015/05/Pirog_2_15.pdf |
| _version_ | 1797422242914631680 |
|---|---|
| author | T. P. Pirog T. A. Shevchuk K. A. Beregova N. V. Kudrya |
| author_facet | T. P. Pirog T. A. Shevchuk K. A. Beregova N. V. Kudrya |
| author_sort | T. P. Pirog |
| collection | DOAJ |
| description | It has been established that in cells of Nocardia vaccinii IMB B-7405 (surfactant producer) glucose catabolism is performed through pentose phosphate cycle as well as through gluconate (activity of NAD+-dependent glucose-6- phosphate dehydrogenase and FAD+-dependent glucose dehydrogenase 835 ± 41 and 698 ± 35 nmol∙min-1∙mg-1 of protein respectively). 6-Phosphogluconate formed in the gluconokinase reaction is involved in the pentose phosphate cycle (activity of constitutive NADP+-dependent 6-phosphogluconate dehydrogenase 357 ± 17 nmol∙min-1∙mg-1 of protein). Glycerol catabolism to dihydroxyacetonephosphate (the intermediate of glycolysis) may be performed in two ways: through glycerol-3-phosphate (glycerol kinase activity 244 ± 12 nmol∙min-1∙mg-1 of protein) and through dihydroxyacetone. Replenishment of the C4-dicarboxylic acids pool in N. vaccinii IMV B-7405 grown on glucose and glycerol occurs in the phosphoenolpyruvate(PEP)carboxylase reaction (714–803 nmol∙min-1∙mg-1 of protein). 2-Oxoglutarate was involved in tricarboxylic acid cycle by alternate pathway with the participation of 2-oxoglutarate synthase.
The observed activity of both key enzymes of gluconeogenesis (PEP- carboxykinase and PEP-synthase), trehalose phosphate synthase and NADP+-dependent glutamate dehydrogenase confirmed the ability of IMV B-7405 strain to the synthesis of surface active glyco- and aminolipids, respectively. |
| first_indexed | 2024-03-09T07:30:25Z |
| format | Article |
| id | doaj.art-8ea00cf3a08c4c5dbde5012514e67346 |
| institution | Directory Open Access Journal |
| issn | 2409-4943 2413-5003 |
| language | English |
| last_indexed | 2024-03-09T07:30:25Z |
| publishDate | 2015-04-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | The Ukrainian Biochemical Journal |
| spelling | doaj.art-8ea00cf3a08c4c5dbde5012514e673462023-12-03T06:27:17ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432413-50032015-04-01872667510.15407/ubj87.02.066Peculiarities of glucose and glycerol metabolism in Nocardia vaccinii IMB B-7405T. P. Pirog0T. A. Shevchuk1K. A. Beregova2N. V. Kudrya3Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kiyv; National University of Food Technologies, Kiyv, UkraineZabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, KiyvNational University of Food Technologies, Kiyv, UkraineNational University of Food Technologies, Kiyv, UkraineIt has been established that in cells of Nocardia vaccinii IMB B-7405 (surfactant producer) glucose catabolism is performed through pentose phosphate cycle as well as through gluconate (activity of NAD+-dependent glucose-6- phosphate dehydrogenase and FAD+-dependent glucose dehydrogenase 835 ± 41 and 698 ± 35 nmol∙min-1∙mg-1 of protein respectively). 6-Phosphogluconate formed in the gluconokinase reaction is involved in the pentose phosphate cycle (activity of constitutive NADP+-dependent 6-phosphogluconate dehydrogenase 357 ± 17 nmol∙min-1∙mg-1 of protein). Glycerol catabolism to dihydroxyacetonephosphate (the intermediate of glycolysis) may be performed in two ways: through glycerol-3-phosphate (glycerol kinase activity 244 ± 12 nmol∙min-1∙mg-1 of protein) and through dihydroxyacetone. Replenishment of the C4-dicarboxylic acids pool in N. vaccinii IMV B-7405 grown on glucose and glycerol occurs in the phosphoenolpyruvate(PEP)carboxylase reaction (714–803 nmol∙min-1∙mg-1 of protein). 2-Oxoglutarate was involved in tricarboxylic acid cycle by alternate pathway with the participation of 2-oxoglutarate synthase. The observed activity of both key enzymes of gluconeogenesis (PEP- carboxykinase and PEP-synthase), trehalose phosphate synthase and NADP+-dependent glutamate dehydrogenase confirmed the ability of IMV B-7405 strain to the synthesis of surface active glyco- and aminolipids, respectively.http://ukrbiochemjournal.org/wp-content/uploads/2015/05/Pirog_2_15.pdfactivity of enzymesanaplerotic reactionsbiosynthesiscatabolism of glucose and glycerolkey enzymesNocardia vaccinii IMB B-7405 |
| spellingShingle | T. P. Pirog T. A. Shevchuk K. A. Beregova N. V. Kudrya Peculiarities of glucose and glycerol metabolism in Nocardia vaccinii IMB B-7405 The Ukrainian Biochemical Journal activity of enzymes anaplerotic reactions biosynthesis catabolism of glucose and glycerol key enzymes Nocardia vaccinii IMB B-7405 |
| title | Peculiarities of glucose and glycerol metabolism in Nocardia vaccinii IMB B-7405 |
| title_full | Peculiarities of glucose and glycerol metabolism in Nocardia vaccinii IMB B-7405 |
| title_fullStr | Peculiarities of glucose and glycerol metabolism in Nocardia vaccinii IMB B-7405 |
| title_full_unstemmed | Peculiarities of glucose and glycerol metabolism in Nocardia vaccinii IMB B-7405 |
| title_short | Peculiarities of glucose and glycerol metabolism in Nocardia vaccinii IMB B-7405 |
| title_sort | peculiarities of glucose and glycerol metabolism in nocardia vaccinii imb b 7405 |
| topic | activity of enzymes anaplerotic reactions biosynthesis catabolism of glucose and glycerol key enzymes Nocardia vaccinii IMB B-7405 |
| url | http://ukrbiochemjournal.org/wp-content/uploads/2015/05/Pirog_2_15.pdf |
| work_keys_str_mv | AT tppirog peculiaritiesofglucoseandglycerolmetabolisminnocardiavacciniiimbb7405 AT tashevchuk peculiaritiesofglucoseandglycerolmetabolisminnocardiavacciniiimbb7405 AT kaberegova peculiaritiesofglucoseandglycerolmetabolisminnocardiavacciniiimbb7405 AT nvkudrya peculiaritiesofglucoseandglycerolmetabolisminnocardiavacciniiimbb7405 |