Immunological Effects of Cold Atmospheric Plasma-Treated Cells in Comparison with Those of Cells Treated with Lactaptin-Based Anticancer Drugs
The ability of dying cancer cells to induce an anticancer immune response can increase the effectiveness of anticancer therapies, and such type of death is termed immunogenic cell death (ICD). Cells can die along the ICD pathway when exposed not only to chemo- and immunotherapeutics, but also to var...
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MDPI AG
2022-09-01
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| Series: | Biophysica |
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| Online Access: | https://www.mdpi.com/2673-4125/2/3/25 |
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| author | Olga Troitskaya Diana Novak Mikhail Varlamov Mikhail Biryukov Anna Nushtaeva Galina Kochneva Dmitriy Zakrevsky Irina Schweigert Vladimir Richter Olga Koval |
| author_facet | Olga Troitskaya Diana Novak Mikhail Varlamov Mikhail Biryukov Anna Nushtaeva Galina Kochneva Dmitriy Zakrevsky Irina Schweigert Vladimir Richter Olga Koval |
| author_sort | Olga Troitskaya |
| collection | DOAJ |
| description | The ability of dying cancer cells to induce an anticancer immune response can increase the effectiveness of anticancer therapies, and such type of death is termed immunogenic cell death (ICD). Cells can die along the ICD pathway when exposed not only to chemo- and immunotherapeutics, but also to various types of radiation, such as ionizing radiation and cold atmospheric plasma jets (CAP). We have previously shown that CAP, lactaptin, and a recombinant vaccinia virus encoding lactaptin induce in vitro molecular changes typical of ICD in cancer cells. In the current work, we treated MX-7 rhabdomyosarcoma cells with CAP and lactaptin-based anticancer drugs and evaluated the immunological effects of the treated cells. We showed that dendritic cells (DCs) captured cells treated with various ICD inducers with different efficiency. CAP-treated cells were weakly potent in inducing the maturation of DCs according to MHC II externalization. Moreover, CAP-treated cells were worse in the stimulation of IFN-α release in vitro and were poorly captured by spleen DCs in vivo. Under the irradiation conditions used, CAP was not capable of activating a significant immunological anti-tumor effect in vivo. It is possible that modifications of the CAP irradiation regimen will enhance the activation of the immune system. |
| first_indexed | 2024-03-10T00:35:31Z |
| format | Article |
| id | doaj.art-8ea3134953e2491d83dfc883020d34cb |
| institution | Directory Open Access Journal |
| issn | 2673-4125 |
| language | English |
| last_indexed | 2024-03-10T00:35:31Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biophysica |
| spelling | doaj.art-8ea3134953e2491d83dfc883020d34cb2023-11-23T15:17:02ZengMDPI AGBiophysica2673-41252022-09-012326628010.3390/biophysica2030025Immunological Effects of Cold Atmospheric Plasma-Treated Cells in Comparison with Those of Cells Treated with Lactaptin-Based Anticancer DrugsOlga Troitskaya0Diana Novak1Mikhail Varlamov2Mikhail Biryukov3Anna Nushtaeva4Galina Kochneva5Dmitriy Zakrevsky6Irina Schweigert7Vladimir Richter8Olga Koval9Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, RussiaState Research Center of Virology and Biotechnology “Vector”, 630559 Koltsovo, RussiaRzhanov Institute of Semiconductor Physic, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, RussiaKhristianovich Institute of Theoretical and Applied Mechanics, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, RussiaThe ability of dying cancer cells to induce an anticancer immune response can increase the effectiveness of anticancer therapies, and such type of death is termed immunogenic cell death (ICD). Cells can die along the ICD pathway when exposed not only to chemo- and immunotherapeutics, but also to various types of radiation, such as ionizing radiation and cold atmospheric plasma jets (CAP). We have previously shown that CAP, lactaptin, and a recombinant vaccinia virus encoding lactaptin induce in vitro molecular changes typical of ICD in cancer cells. In the current work, we treated MX-7 rhabdomyosarcoma cells with CAP and lactaptin-based anticancer drugs and evaluated the immunological effects of the treated cells. We showed that dendritic cells (DCs) captured cells treated with various ICD inducers with different efficiency. CAP-treated cells were weakly potent in inducing the maturation of DCs according to MHC II externalization. Moreover, CAP-treated cells were worse in the stimulation of IFN-α release in vitro and were poorly captured by spleen DCs in vivo. Under the irradiation conditions used, CAP was not capable of activating a significant immunological anti-tumor effect in vivo. It is possible that modifications of the CAP irradiation regimen will enhance the activation of the immune system.https://www.mdpi.com/2673-4125/2/3/25cold plasma jetimmunogenic cell deathdendritic cellsinterferon |
| spellingShingle | Olga Troitskaya Diana Novak Mikhail Varlamov Mikhail Biryukov Anna Nushtaeva Galina Kochneva Dmitriy Zakrevsky Irina Schweigert Vladimir Richter Olga Koval Immunological Effects of Cold Atmospheric Plasma-Treated Cells in Comparison with Those of Cells Treated with Lactaptin-Based Anticancer Drugs Biophysica cold plasma jet immunogenic cell death dendritic cells interferon |
| title | Immunological Effects of Cold Atmospheric Plasma-Treated Cells in Comparison with Those of Cells Treated with Lactaptin-Based Anticancer Drugs |
| title_full | Immunological Effects of Cold Atmospheric Plasma-Treated Cells in Comparison with Those of Cells Treated with Lactaptin-Based Anticancer Drugs |
| title_fullStr | Immunological Effects of Cold Atmospheric Plasma-Treated Cells in Comparison with Those of Cells Treated with Lactaptin-Based Anticancer Drugs |
| title_full_unstemmed | Immunological Effects of Cold Atmospheric Plasma-Treated Cells in Comparison with Those of Cells Treated with Lactaptin-Based Anticancer Drugs |
| title_short | Immunological Effects of Cold Atmospheric Plasma-Treated Cells in Comparison with Those of Cells Treated with Lactaptin-Based Anticancer Drugs |
| title_sort | immunological effects of cold atmospheric plasma treated cells in comparison with those of cells treated with lactaptin based anticancer drugs |
| topic | cold plasma jet immunogenic cell death dendritic cells interferon |
| url | https://www.mdpi.com/2673-4125/2/3/25 |
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