Exploring Cancer Cell Behavior In Vitro in Three-Dimensional Multicellular Bioprintable Collagen-Based Hydrogels
In vitro cancer 3D models are valuable tools to provide mechanistic insight into solid tumor growth, invasion, and drug delivery. The 3D spheroid model of solid tumors has been the most popular cancer model in use until now. However, previous studies have shown that these spheroid models lack suffic...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-02-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/11/2/180 |
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| author | Daniela F. Duarte Campos Andrea Bonnin Marquez Cathal O’Seanain Horst Fischer Andreas Blaeser Michael Vogt Diana Corallo Sanja Aveic |
| author_facet | Daniela F. Duarte Campos Andrea Bonnin Marquez Cathal O’Seanain Horst Fischer Andreas Blaeser Michael Vogt Diana Corallo Sanja Aveic |
| author_sort | Daniela F. Duarte Campos |
| collection | DOAJ |
| description | In vitro cancer 3D models are valuable tools to provide mechanistic insight into solid tumor growth, invasion, and drug delivery. The 3D spheroid model of solid tumors has been the most popular cancer model in use until now. However, previous studies have shown that these spheroid models lack sufficient morphological parameters, which may affect their response to chemicals. In this work, we proposed the fabrication of miniaturized 3D cancer models using collagen type I-based bioprintable bioinks. In the context of a mimicking model for advanced neuroblastoma studies, we showed that cancer cells contained in bioprintable bioinks formed Homer Wright-like rosettes, maintained their proliferative capacities and produced an equivalent Vimentin-rich matrix unlike that of non-bioprintable bioinks which made for poorer models. In addition, bioprintable bioinks were successfully bioprinted as compartmentalized 3D models in the centimeter scale, which was not feasible using non-bioprintable bioinks. In contrast to non-bioprintable hydrogels, we did not observe contraction in their bioprintable counterparts, which is an advantage for prospective 3D bioprinted models that should attain stable rheological and mechanical properties after bioprinting. By adopting this proposed system for the use of patient-derived primary tumor cells, the approach could be introduced as a first line strategy in precision medicine for testing the response of neuroblastoma cells to drugs, especially when disease progresses rapidly or patients do not respond to actual therapy regimens. |
| first_indexed | 2024-03-12T10:50:43Z |
| format | Article |
| id | doaj.art-8ea5667c949b4654a5cce36b58aaeae9 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-12T10:50:43Z |
| publishDate | 2019-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-8ea5667c949b4654a5cce36b58aaeae92023-09-02T07:01:49ZengMDPI AGCancers2072-66942019-02-0111218010.3390/cancers11020180cancers11020180Exploring Cancer Cell Behavior In Vitro in Three-Dimensional Multicellular Bioprintable Collagen-Based HydrogelsDaniela F. Duarte Campos0Andrea Bonnin Marquez1Cathal O’Seanain2Horst Fischer3Andreas Blaeser4Michael Vogt5Diana Corallo6Sanja Aveic7Department of Dental Materials and Biomaterials Research, RWTH Aachen University Hospital, 52074 Aachen, GermanyDepartment of Dental Materials and Biomaterials Research, RWTH Aachen University Hospital, 52074 Aachen, GermanyDepartment of Dental Materials and Biomaterials Research, RWTH Aachen University Hospital, 52074 Aachen, GermanyDepartment of Dental Materials and Biomaterials Research, RWTH Aachen University Hospital, 52074 Aachen, GermanyMedical Textiles and Biofabrication, Institut fuer Textiltechnik, RWTH Aachen University, 52074 Aachen, GermanyInterdisciplinary Center for Clinical Research, RWTH Aachen University Hospital, 52074 Aachen, GermanyFondazione Instituto di Ricerca Pediatrica Citta’ della Speranza, Neuroblastoma Laboratory, 35127 Padua, ItalyFondazione Instituto di Ricerca Pediatrica Citta’ della Speranza, Neuroblastoma Laboratory, 35127 Padua, ItalyIn vitro cancer 3D models are valuable tools to provide mechanistic insight into solid tumor growth, invasion, and drug delivery. The 3D spheroid model of solid tumors has been the most popular cancer model in use until now. However, previous studies have shown that these spheroid models lack sufficient morphological parameters, which may affect their response to chemicals. In this work, we proposed the fabrication of miniaturized 3D cancer models using collagen type I-based bioprintable bioinks. In the context of a mimicking model for advanced neuroblastoma studies, we showed that cancer cells contained in bioprintable bioinks formed Homer Wright-like rosettes, maintained their proliferative capacities and produced an equivalent Vimentin-rich matrix unlike that of non-bioprintable bioinks which made for poorer models. In addition, bioprintable bioinks were successfully bioprinted as compartmentalized 3D models in the centimeter scale, which was not feasible using non-bioprintable bioinks. In contrast to non-bioprintable hydrogels, we did not observe contraction in their bioprintable counterparts, which is an advantage for prospective 3D bioprinted models that should attain stable rheological and mechanical properties after bioprinting. By adopting this proposed system for the use of patient-derived primary tumor cells, the approach could be introduced as a first line strategy in precision medicine for testing the response of neuroblastoma cells to drugs, especially when disease progresses rapidly or patients do not respond to actual therapy regimens.https://www.mdpi.com/2072-6694/11/2/180cancer cellin vitro modelhydrogel3Dbioprinting |
| spellingShingle | Daniela F. Duarte Campos Andrea Bonnin Marquez Cathal O’Seanain Horst Fischer Andreas Blaeser Michael Vogt Diana Corallo Sanja Aveic Exploring Cancer Cell Behavior In Vitro in Three-Dimensional Multicellular Bioprintable Collagen-Based Hydrogels Cancers cancer cell in vitro model hydrogel 3D bioprinting |
| title | Exploring Cancer Cell Behavior In Vitro in Three-Dimensional Multicellular Bioprintable Collagen-Based Hydrogels |
| title_full | Exploring Cancer Cell Behavior In Vitro in Three-Dimensional Multicellular Bioprintable Collagen-Based Hydrogels |
| title_fullStr | Exploring Cancer Cell Behavior In Vitro in Three-Dimensional Multicellular Bioprintable Collagen-Based Hydrogels |
| title_full_unstemmed | Exploring Cancer Cell Behavior In Vitro in Three-Dimensional Multicellular Bioprintable Collagen-Based Hydrogels |
| title_short | Exploring Cancer Cell Behavior In Vitro in Three-Dimensional Multicellular Bioprintable Collagen-Based Hydrogels |
| title_sort | exploring cancer cell behavior in vitro in three dimensional multicellular bioprintable collagen based hydrogels |
| topic | cancer cell in vitro model hydrogel 3D bioprinting |
| url | https://www.mdpi.com/2072-6694/11/2/180 |
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