The First Whole Genome Sequencing of <i>Agaricus bitorquis</i> and Its Metabolite Profiling
<i>Agaricus bitorquis</i>, an emerging wild mushroom with remarkable biological activities and a distinctive oversized mushroom shape, has gained increasing attention in recent years. Despite its status as an important resource of wild edible fungi, knowledge about this mushroom is still...
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MDPI AG
2023-04-01
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| Series: | Journal of Fungi |
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| author | Chunhua Zhao Xi-long Feng Zhen-xin Wang Jianzhao Qi |
| author_facet | Chunhua Zhao Xi-long Feng Zhen-xin Wang Jianzhao Qi |
| author_sort | Chunhua Zhao |
| collection | DOAJ |
| description | <i>Agaricus bitorquis</i>, an emerging wild mushroom with remarkable biological activities and a distinctive oversized mushroom shape, has gained increasing attention in recent years. Despite its status as an important resource of wild edible fungi, knowledge about this mushroom is still limited. In this study, we used the Illumina NovaSeq and Nanopore PromethION platforms to sequence, de novo assemble, and annotate the whole genome and mitochondrial genome (mitogenome) of the <i>A. bitorquis</i> strain BH01 isolated from Bosten Lake, Xinjiang Province, China. Using the genome-based biological information, we identified candidate genes associated with mating type and carbohydrate-active enzymes in <i>A. bitorquis</i>. Cluster analysis based on P450 of basidiomycetes revealed the types of P450 members of <i>A. bitorquis</i>. Comparative genomic, mitogenomic, and phylogenetic analyses were also performed, revealing interspecific differences and evolutionary features of <i>A. bitorquis</i> and <i>A. bisporus</i>. In addition, the molecular network of metabolites was investigated, highlighting differences in the chemical composition and content of the fruiting bodies of <i>A. bitorquis</i> and <i>A. bisporus</i>. The genome sequencing provides a comprehensive understanding and knowledge of <i>A. bitorquis</i> and the genus <i>Agaricus</i> mushrooms. This work provides valuable insights into the potential for artificial cultivation and molecular breeding of <i>A. bitorquis</i>, which will facilitate the development of <i>A. bitorquis</i> in the field of edible mushrooms and functional food manufacture. |
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| spelling | doaj.art-8ea7cb6bf8204a4a8048c1da95f1c4622023-11-17T19:58:48ZengMDPI AGJournal of Fungi2309-608X2023-04-019448510.3390/jof9040485The First Whole Genome Sequencing of <i>Agaricus bitorquis</i> and Its Metabolite ProfilingChunhua Zhao0Xi-long Feng1Zhen-xin Wang2Jianzhao Qi3Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaShaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling, Xianyang 712100, ChinaShaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling, Xianyang 712100, ChinaShaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling, Xianyang 712100, China<i>Agaricus bitorquis</i>, an emerging wild mushroom with remarkable biological activities and a distinctive oversized mushroom shape, has gained increasing attention in recent years. Despite its status as an important resource of wild edible fungi, knowledge about this mushroom is still limited. In this study, we used the Illumina NovaSeq and Nanopore PromethION platforms to sequence, de novo assemble, and annotate the whole genome and mitochondrial genome (mitogenome) of the <i>A. bitorquis</i> strain BH01 isolated from Bosten Lake, Xinjiang Province, China. Using the genome-based biological information, we identified candidate genes associated with mating type and carbohydrate-active enzymes in <i>A. bitorquis</i>. Cluster analysis based on P450 of basidiomycetes revealed the types of P450 members of <i>A. bitorquis</i>. Comparative genomic, mitogenomic, and phylogenetic analyses were also performed, revealing interspecific differences and evolutionary features of <i>A. bitorquis</i> and <i>A. bisporus</i>. In addition, the molecular network of metabolites was investigated, highlighting differences in the chemical composition and content of the fruiting bodies of <i>A. bitorquis</i> and <i>A. bisporus</i>. The genome sequencing provides a comprehensive understanding and knowledge of <i>A. bitorquis</i> and the genus <i>Agaricus</i> mushrooms. This work provides valuable insights into the potential for artificial cultivation and molecular breeding of <i>A. bitorquis</i>, which will facilitate the development of <i>A. bitorquis</i> in the field of edible mushrooms and functional food manufacture.https://www.mdpi.com/2309-608X/9/4/485<i>Agaricus bitorquis</i>comparative genomemitogenomeedible mushroom |
| spellingShingle | Chunhua Zhao Xi-long Feng Zhen-xin Wang Jianzhao Qi The First Whole Genome Sequencing of <i>Agaricus bitorquis</i> and Its Metabolite Profiling Journal of Fungi <i>Agaricus bitorquis</i> comparative genome mitogenome edible mushroom |
| title | The First Whole Genome Sequencing of <i>Agaricus bitorquis</i> and Its Metabolite Profiling |
| title_full | The First Whole Genome Sequencing of <i>Agaricus bitorquis</i> and Its Metabolite Profiling |
| title_fullStr | The First Whole Genome Sequencing of <i>Agaricus bitorquis</i> and Its Metabolite Profiling |
| title_full_unstemmed | The First Whole Genome Sequencing of <i>Agaricus bitorquis</i> and Its Metabolite Profiling |
| title_short | The First Whole Genome Sequencing of <i>Agaricus bitorquis</i> and Its Metabolite Profiling |
| title_sort | first whole genome sequencing of i agaricus bitorquis i and its metabolite profiling |
| topic | <i>Agaricus bitorquis</i> comparative genome mitogenome edible mushroom |
| url | https://www.mdpi.com/2309-608X/9/4/485 |
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