Seed Transmission of Begomoviruses: A Potential Threat for Bitter Gourd Cultivation
Bitter gourd (<i>Momordica charantia</i> L.), one of the valued vegetable crops in India, is severely affected by yellow mosaic disease caused by two begomoviruses, tomato leaf curl New Delhi virus (ToLCNDV) and bitter gourd yellow mosaic virus (BgYMV). The symptoms are yellowing, distor...
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MDPI AG
2023-03-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/6/1396 |
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| author | Ravisankar Gomathi Devi Chinnaraj Jothika Arjunan Sankari Sethuraman Lakshmi Varagur Ganesan Malathi Perumal Renukadevi |
| author_facet | Ravisankar Gomathi Devi Chinnaraj Jothika Arjunan Sankari Sethuraman Lakshmi Varagur Ganesan Malathi Perumal Renukadevi |
| author_sort | Ravisankar Gomathi Devi |
| collection | DOAJ |
| description | Bitter gourd (<i>Momordica charantia</i> L.), one of the valued vegetable crops in India, is severely affected by yellow mosaic disease caused by two begomoviruses, tomato leaf curl New Delhi virus (ToLCNDV) and bitter gourd yellow mosaic virus (BgYMV). The symptoms are yellowing, distortion of leaf, puckering, and malformed fruits. Increased incidence of the disease and appearance of symptoms even in young emerging seedling stage were suggestive of seed transmission of the viruses, which was examined in detail. To study the seed transmission, two sources—seeds of elite hybrids H1, H2, H3, H4, and Co1 procured from a seed market; and seeds from infected plants in the farmer’s field were tested. Detection of the virus by DAS-ELISA using polyclonal antibody indicated embryo infection up to 63%, 26%, 20%, and 10% in hybrids H1, H2, H3, and H4, respectively, for market-procured seeds. In PCR analysis with primers specific for ToLCNDV and BgYMV, infection by ToLCNDV was as high as 76% and mixed infection was 24%. In contrast, in seeds derived from field-infected plants, the percentage detection was less. Grow-out tests with market-procured seeds revealed no transmission for BgYMV compared with 5% transmission for ToLCNDV. Whether seed-borne inocula could serve as an inoculum for new infection in a field and further progress of the disease was investigated in a microplot study. The study clearly revealed variation in seed transmission between different sources, lots, cultivars, and viruses. The virus present in symptomatic and asymptomatic plants was easily transmitted by whitefly. In another microplot experiment, the potential of seed-borne virus as inoculum was proved. There was 43.3% initial seed transmission in the microplot, increasing to 70% after release of 60 whiteflies. |
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| language | English |
| last_indexed | 2024-03-11T06:00:23Z |
| publishDate | 2023-03-01 |
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| series | Plants |
| spelling | doaj.art-8ea978883587443cb04b0d34c12a56f52023-11-17T13:23:20ZengMDPI AGPlants2223-77472023-03-01126139610.3390/plants12061396Seed Transmission of Begomoviruses: A Potential Threat for Bitter Gourd CultivationRavisankar Gomathi Devi0Chinnaraj Jothika1Arjunan Sankari2Sethuraman Lakshmi3Varagur Ganesan Malathi4Perumal Renukadevi5Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore 641003, Tamil Nadu, IndiaDepartment of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore 641003, Tamil Nadu, IndiaDepartment of Vegetable Science, Tamil Nadu Agricultural University, Coimbatore 641003, Tamil Nadu, IndiaDepartment of Seed Science and Technology, Tamil Nadu Agricultural University, Coimbatore 641003, Tamil Nadu, IndiaRetired Scientist, ICAR-IARI, GI, Sree Kumaran Hill Crest Apartment, Coimbatore 641046, Tamil Nadu, IndiaDepartment of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore 641003, Tamil Nadu, IndiaBitter gourd (<i>Momordica charantia</i> L.), one of the valued vegetable crops in India, is severely affected by yellow mosaic disease caused by two begomoviruses, tomato leaf curl New Delhi virus (ToLCNDV) and bitter gourd yellow mosaic virus (BgYMV). The symptoms are yellowing, distortion of leaf, puckering, and malformed fruits. Increased incidence of the disease and appearance of symptoms even in young emerging seedling stage were suggestive of seed transmission of the viruses, which was examined in detail. To study the seed transmission, two sources—seeds of elite hybrids H1, H2, H3, H4, and Co1 procured from a seed market; and seeds from infected plants in the farmer’s field were tested. Detection of the virus by DAS-ELISA using polyclonal antibody indicated embryo infection up to 63%, 26%, 20%, and 10% in hybrids H1, H2, H3, and H4, respectively, for market-procured seeds. In PCR analysis with primers specific for ToLCNDV and BgYMV, infection by ToLCNDV was as high as 76% and mixed infection was 24%. In contrast, in seeds derived from field-infected plants, the percentage detection was less. Grow-out tests with market-procured seeds revealed no transmission for BgYMV compared with 5% transmission for ToLCNDV. Whether seed-borne inocula could serve as an inoculum for new infection in a field and further progress of the disease was investigated in a microplot study. The study clearly revealed variation in seed transmission between different sources, lots, cultivars, and viruses. The virus present in symptomatic and asymptomatic plants was easily transmitted by whitefly. In another microplot experiment, the potential of seed-borne virus as inoculum was proved. There was 43.3% initial seed transmission in the microplot, increasing to 70% after release of 60 whiteflies.https://www.mdpi.com/2223-7747/12/6/1396<i>Momordica charantia</i> L. embryogrow-out testmicroplotToLCNDVBgYMV |
| spellingShingle | Ravisankar Gomathi Devi Chinnaraj Jothika Arjunan Sankari Sethuraman Lakshmi Varagur Ganesan Malathi Perumal Renukadevi Seed Transmission of Begomoviruses: A Potential Threat for Bitter Gourd Cultivation Plants <i>Momordica charantia</i> L. embryo grow-out test microplot ToLCNDV BgYMV |
| title | Seed Transmission of Begomoviruses: A Potential Threat for Bitter Gourd Cultivation |
| title_full | Seed Transmission of Begomoviruses: A Potential Threat for Bitter Gourd Cultivation |
| title_fullStr | Seed Transmission of Begomoviruses: A Potential Threat for Bitter Gourd Cultivation |
| title_full_unstemmed | Seed Transmission of Begomoviruses: A Potential Threat for Bitter Gourd Cultivation |
| title_short | Seed Transmission of Begomoviruses: A Potential Threat for Bitter Gourd Cultivation |
| title_sort | seed transmission of begomoviruses a potential threat for bitter gourd cultivation |
| topic | <i>Momordica charantia</i> L. embryo grow-out test microplot ToLCNDV BgYMV |
| url | https://www.mdpi.com/2223-7747/12/6/1396 |
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