Impact of Four Protein Additives in Cryogels on Osteogenic Differentiation of Adipose-Derived Mesenchymal Stem Cells
Human adipose-derived mesenchymal stem/stromal cells (Ad-MSCs) have great potential for bone tissue engineering. Cryogels, mimicking the three-dimensional structure of spongy bone, represent ideal carriers for these cells. We developed poly(2-hydroxyethyl methacrylate) cryogels, containing hydroxyap...
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| Format: | Article |
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MDPI AG
2019-08-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/6/3/67 |
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| author | Victor Häussling Sebastian Deninger Laura Vidoni Helen Rinderknecht Marc Ruoß Christian Arnscheidt Kiriaki Athanasopulu Ralf Kemkemer Andreas K. Nussler Sabrina Ehnert |
| author_facet | Victor Häussling Sebastian Deninger Laura Vidoni Helen Rinderknecht Marc Ruoß Christian Arnscheidt Kiriaki Athanasopulu Ralf Kemkemer Andreas K. Nussler Sabrina Ehnert |
| author_sort | Victor Häussling |
| collection | DOAJ |
| description | Human adipose-derived mesenchymal stem/stromal cells (Ad-MSCs) have great potential for bone tissue engineering. Cryogels, mimicking the three-dimensional structure of spongy bone, represent ideal carriers for these cells. We developed poly(2-hydroxyethyl methacrylate) cryogels, containing hydroxyapatite to mimic inorganic bone matrix. Cryogels were additionally supplemented with different types of proteins, namely collagen (Coll), platelet-rich plasma (PRP), immune cells-conditioned medium (CM), and RGD peptides (RGD). The different protein components did not affect scaffolds’ porosity or water-uptake capacity, but altered pore size and stiffness. Stiffness was highest in scaffolds with PRP (82.3 kPa), followed by Coll (55.3 kPa), CM (45.6 kPa), and RGD (32.8 kPa). Scaffolds with PRP, CM, and Coll had the largest pore diameters (~60 µm). Ad-MSCs were osteogenically differentiated on these scaffolds for 14 days. Cell attachment and survival rates were comparable for all four scaffolds. Runx2 and osteocalcin levels only increased in Ad-MSCs on Coll, PRP and CM cryogels. Osterix levels increased slightly in Ad-MSCs differentiated on Coll and PRP cryogels. With differentiation alkaline phosphatase activity decreased under all four conditions. In summary, besides Coll cryogel our PRP cryogel constitutes as an especially suitable carrier for bone tissue engineering. This is of special interest, as this scaffold can be generated with patients’ PRP. |
| first_indexed | 2024-03-12T19:54:05Z |
| format | Article |
| id | doaj.art-8eac14f953a94b4393f9b7787a4e3787 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-12T19:54:05Z |
| publishDate | 2019-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-8eac14f953a94b4393f9b7787a4e37872023-08-02T02:54:32ZengMDPI AGBioengineering2306-53542019-08-01636710.3390/bioengineering6030067bioengineering6030067Impact of Four Protein Additives in Cryogels on Osteogenic Differentiation of Adipose-Derived Mesenchymal Stem CellsVictor Häussling0Sebastian Deninger1Laura Vidoni2Helen Rinderknecht3Marc Ruoß4Christian Arnscheidt5Kiriaki Athanasopulu6Ralf Kemkemer7Andreas K. Nussler8Sabrina Ehnert9Siegfried Weller Research Institute, BG Unfallklinik Tuebingen, Department of Trauma and Reconstructive Surgery, Eberhard Karls University Tübingen, 72074 Tübingen, GermanySiegfried Weller Research Institute, BG Unfallklinik Tuebingen, Department of Trauma and Reconstructive Surgery, Eberhard Karls University Tübingen, 72074 Tübingen, GermanySiegfried Weller Research Institute, BG Unfallklinik Tuebingen, Department of Trauma and Reconstructive Surgery, Eberhard Karls University Tübingen, 72074 Tübingen, GermanySiegfried Weller Research Institute, BG Unfallklinik Tuebingen, Department of Trauma and Reconstructive Surgery, Eberhard Karls University Tübingen, 72074 Tübingen, GermanySiegfried Weller Research Institute, BG Unfallklinik Tuebingen, Department of Trauma and Reconstructive Surgery, Eberhard Karls University Tübingen, 72074 Tübingen, GermanySiegfried Weller Research Institute, BG Unfallklinik Tuebingen, Department of Trauma and Reconstructive Surgery, Eberhard Karls University Tübingen, 72074 Tübingen, GermanyDepartment of Applied Chemistry Reutlingen University, 72762 Reutlingen, GermanyDepartment of Applied Chemistry Reutlingen University, 72762 Reutlingen, GermanySiegfried Weller Research Institute, BG Unfallklinik Tuebingen, Department of Trauma and Reconstructive Surgery, Eberhard Karls University Tübingen, 72074 Tübingen, GermanySiegfried Weller Research Institute, BG Unfallklinik Tuebingen, Department of Trauma and Reconstructive Surgery, Eberhard Karls University Tübingen, 72074 Tübingen, GermanyHuman adipose-derived mesenchymal stem/stromal cells (Ad-MSCs) have great potential for bone tissue engineering. Cryogels, mimicking the three-dimensional structure of spongy bone, represent ideal carriers for these cells. We developed poly(2-hydroxyethyl methacrylate) cryogels, containing hydroxyapatite to mimic inorganic bone matrix. Cryogels were additionally supplemented with different types of proteins, namely collagen (Coll), platelet-rich plasma (PRP), immune cells-conditioned medium (CM), and RGD peptides (RGD). The different protein components did not affect scaffolds’ porosity or water-uptake capacity, but altered pore size and stiffness. Stiffness was highest in scaffolds with PRP (82.3 kPa), followed by Coll (55.3 kPa), CM (45.6 kPa), and RGD (32.8 kPa). Scaffolds with PRP, CM, and Coll had the largest pore diameters (~60 µm). Ad-MSCs were osteogenically differentiated on these scaffolds for 14 days. Cell attachment and survival rates were comparable for all four scaffolds. Runx2 and osteocalcin levels only increased in Ad-MSCs on Coll, PRP and CM cryogels. Osterix levels increased slightly in Ad-MSCs differentiated on Coll and PRP cryogels. With differentiation alkaline phosphatase activity decreased under all four conditions. In summary, besides Coll cryogel our PRP cryogel constitutes as an especially suitable carrier for bone tissue engineering. This is of special interest, as this scaffold can be generated with patients’ PRP.https://www.mdpi.com/2306-5354/6/3/67bone tissue engineeringcryogeladipose-derived mesenchymal stem/stromal cells (Ad-MSCs)3D-culturescaffoldplatelet-rich plasma (PRP)RGDcollagenimmune-cell conditioned medium |
| spellingShingle | Victor Häussling Sebastian Deninger Laura Vidoni Helen Rinderknecht Marc Ruoß Christian Arnscheidt Kiriaki Athanasopulu Ralf Kemkemer Andreas K. Nussler Sabrina Ehnert Impact of Four Protein Additives in Cryogels on Osteogenic Differentiation of Adipose-Derived Mesenchymal Stem Cells Bioengineering bone tissue engineering cryogel adipose-derived mesenchymal stem/stromal cells (Ad-MSCs) 3D-culture scaffold platelet-rich plasma (PRP) RGD collagen immune-cell conditioned medium |
| title | Impact of Four Protein Additives in Cryogels on Osteogenic Differentiation of Adipose-Derived Mesenchymal Stem Cells |
| title_full | Impact of Four Protein Additives in Cryogels on Osteogenic Differentiation of Adipose-Derived Mesenchymal Stem Cells |
| title_fullStr | Impact of Four Protein Additives in Cryogels on Osteogenic Differentiation of Adipose-Derived Mesenchymal Stem Cells |
| title_full_unstemmed | Impact of Four Protein Additives in Cryogels on Osteogenic Differentiation of Adipose-Derived Mesenchymal Stem Cells |
| title_short | Impact of Four Protein Additives in Cryogels on Osteogenic Differentiation of Adipose-Derived Mesenchymal Stem Cells |
| title_sort | impact of four protein additives in cryogels on osteogenic differentiation of adipose derived mesenchymal stem cells |
| topic | bone tissue engineering cryogel adipose-derived mesenchymal stem/stromal cells (Ad-MSCs) 3D-culture scaffold platelet-rich plasma (PRP) RGD collagen immune-cell conditioned medium |
| url | https://www.mdpi.com/2306-5354/6/3/67 |
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