Effect of a Stannous Fluoride Dentifrice on Biofilm Composition, Gene Expression and Biomechanical Properties
An in situ study was conducted to examine the mode of action of a 0.454% stannous fluoride (SnF<sub>2</sub>)-containing dentifrice in controlling the composition and properties of oral biofilm. Thirteen generally healthy individuals participated in the study. Each participant wore an int...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-08-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/10/9/1691 |
| _version_ | 1827658675149340672 |
|---|---|
| author | Hardeep Kaur Gumber Artemis S. Louyakis Tulika Sarma Kristina Ivana Fabijanic Reeba Paul Kristen Mellenbruch Latonya Kilpatrick-Liverman |
| author_facet | Hardeep Kaur Gumber Artemis S. Louyakis Tulika Sarma Kristina Ivana Fabijanic Reeba Paul Kristen Mellenbruch Latonya Kilpatrick-Liverman |
| author_sort | Hardeep Kaur Gumber |
| collection | DOAJ |
| description | An in situ study was conducted to examine the mode of action of a 0.454% stannous fluoride (SnF<sub>2</sub>)-containing dentifrice in controlling the composition and properties of oral biofilm. Thirteen generally healthy individuals participated in the study. Each participant wore an intra-oral appliance over a 48-h period to measure differences in the resulting biofilm’s architecture, mechanical properties, and bacterial composition after using two different toothpaste products. In addition, metatranscriptomics analysis of supragingival plaque was conducted to identify the gene pathways influenced. The thickness and volume of the microcolonies formed when brushing with the SnF<sub>2</sub> dentifrice were dramatically reduced compared to the control 0.76% sodium monofluorophosphate (MFP)-containing toothpaste. Similarly, the biophysical and nanomechanical properties measured by atomic force microscopy (AFM) demonstrated a significant reduction in biofilm adhesive properties. Metatranscriptomic analysis identified pathways associated with biofilm formation, cell adhesion, quorum sensing, and N-glycosylation that are significantly downregulated with SnF<sub>2</sub>. This study provides a clinically relevant snapshot of how the use of a stabilized, SnF<sub>2</sub> toothpaste formulation can change the spatial organization, nanomechanical, and gene expression properties of bacterial communities. |
| first_indexed | 2024-03-09T23:07:01Z |
| format | Article |
| id | doaj.art-8ead181de7a94092a0307603b9f85a4d |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T23:07:01Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-8ead181de7a94092a0307603b9f85a4d2023-11-23T17:51:36ZengMDPI AGMicroorganisms2076-26072022-08-01109169110.3390/microorganisms10091691Effect of a Stannous Fluoride Dentifrice on Biofilm Composition, Gene Expression and Biomechanical PropertiesHardeep Kaur Gumber0Artemis S. Louyakis1Tulika Sarma2Kristina Ivana Fabijanic3Reeba Paul4Kristen Mellenbruch5Latonya Kilpatrick-Liverman6Colgate-Palmolive Technology Center, Piscataway, NJ 08854, USAColgate-Palmolive Technology Center, Piscataway, NJ 08854, USAColgate-Palmolive Technology Center, Piscataway, NJ 08854, USAColgate-Palmolive Technology Center, Piscataway, NJ 08854, USAColgate-Palmolive Technology Center, Piscataway, NJ 08854, USAColgate-Palmolive Technology Center, Piscataway, NJ 08854, USAColgate-Palmolive Technology Center, Piscataway, NJ 08854, USAAn in situ study was conducted to examine the mode of action of a 0.454% stannous fluoride (SnF<sub>2</sub>)-containing dentifrice in controlling the composition and properties of oral biofilm. Thirteen generally healthy individuals participated in the study. Each participant wore an intra-oral appliance over a 48-h period to measure differences in the resulting biofilm’s architecture, mechanical properties, and bacterial composition after using two different toothpaste products. In addition, metatranscriptomics analysis of supragingival plaque was conducted to identify the gene pathways influenced. The thickness and volume of the microcolonies formed when brushing with the SnF<sub>2</sub> dentifrice were dramatically reduced compared to the control 0.76% sodium monofluorophosphate (MFP)-containing toothpaste. Similarly, the biophysical and nanomechanical properties measured by atomic force microscopy (AFM) demonstrated a significant reduction in biofilm adhesive properties. Metatranscriptomic analysis identified pathways associated with biofilm formation, cell adhesion, quorum sensing, and N-glycosylation that are significantly downregulated with SnF<sub>2</sub>. This study provides a clinically relevant snapshot of how the use of a stabilized, SnF<sub>2</sub> toothpaste formulation can change the spatial organization, nanomechanical, and gene expression properties of bacterial communities.https://www.mdpi.com/2076-2607/10/9/1691oral biofilmstannous fluoridedental plaqueoral healthoral microbiometoothpaste |
| spellingShingle | Hardeep Kaur Gumber Artemis S. Louyakis Tulika Sarma Kristina Ivana Fabijanic Reeba Paul Kristen Mellenbruch Latonya Kilpatrick-Liverman Effect of a Stannous Fluoride Dentifrice on Biofilm Composition, Gene Expression and Biomechanical Properties Microorganisms oral biofilm stannous fluoride dental plaque oral health oral microbiome toothpaste |
| title | Effect of a Stannous Fluoride Dentifrice on Biofilm Composition, Gene Expression and Biomechanical Properties |
| title_full | Effect of a Stannous Fluoride Dentifrice on Biofilm Composition, Gene Expression and Biomechanical Properties |
| title_fullStr | Effect of a Stannous Fluoride Dentifrice on Biofilm Composition, Gene Expression and Biomechanical Properties |
| title_full_unstemmed | Effect of a Stannous Fluoride Dentifrice on Biofilm Composition, Gene Expression and Biomechanical Properties |
| title_short | Effect of a Stannous Fluoride Dentifrice on Biofilm Composition, Gene Expression and Biomechanical Properties |
| title_sort | effect of a stannous fluoride dentifrice on biofilm composition gene expression and biomechanical properties |
| topic | oral biofilm stannous fluoride dental plaque oral health oral microbiome toothpaste |
| url | https://www.mdpi.com/2076-2607/10/9/1691 |
| work_keys_str_mv | AT hardeepkaurgumber effectofastannousfluoridedentifriceonbiofilmcompositiongeneexpressionandbiomechanicalproperties AT artemisslouyakis effectofastannousfluoridedentifriceonbiofilmcompositiongeneexpressionandbiomechanicalproperties AT tulikasarma effectofastannousfluoridedentifriceonbiofilmcompositiongeneexpressionandbiomechanicalproperties AT kristinaivanafabijanic effectofastannousfluoridedentifriceonbiofilmcompositiongeneexpressionandbiomechanicalproperties AT reebapaul effectofastannousfluoridedentifriceonbiofilmcompositiongeneexpressionandbiomechanicalproperties AT kristenmellenbruch effectofastannousfluoridedentifriceonbiofilmcompositiongeneexpressionandbiomechanicalproperties AT latonyakilpatrickliverman effectofastannousfluoridedentifriceonbiofilmcompositiongeneexpressionandbiomechanicalproperties |