Capillary electrophoresis as a tool for genotyping SH3 mediated coffee leaf rust resistance
Coffee is an important agricultural commodity in the world. However, it is susceptible to Hemileia vastatrix (Hv), an obligatory biotrophic fungus that causes coffee leaf rust (CLR). Natural resistance to rust has been identified in the wild species Coffea canephora and Coffea liberica. These specie...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Universidad Nacional de Trujillo
2021-03-01
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| Series: | Scientia Agropecuaria |
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| Online Access: | http://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/3340 |
| _version_ | 1818948569701285888 |
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| author | Savina A. Gutiérrez-Calle Rosa A. Sánchez-Díaz Yolanda B. Delgado-Silva Juan D. Montenegro Dina L. Gutiérrez Jorge L. Maicelo-Quintana Juan C. Guerrero-Abad |
| author_facet | Savina A. Gutiérrez-Calle Rosa A. Sánchez-Díaz Yolanda B. Delgado-Silva Juan D. Montenegro Dina L. Gutiérrez Jorge L. Maicelo-Quintana Juan C. Guerrero-Abad |
| author_sort | Savina A. Gutiérrez-Calle |
| collection | DOAJ |
| description | Coffee is an important agricultural commodity in the world. However, it is susceptible to Hemileia vastatrix (Hv), an obligatory biotrophic fungus that causes coffee leaf rust (CLR). Natural resistance to rust has been identified in the wild species Coffea canephora and Coffea liberica. These species have been used in breeding programs where interspecific resistant hybrids have been generated. The SH3 gene, derived from C. liberica, has been shown to confer extreme and long-lasting resistance to Hv. A total of 167 accessions of the INIA’s Coffee Germplasm Collection of Peru (INIA-CGC) were screened with 4 markers linked to the SH3 gene. As positive controls, EA67 (C. liberica) and the hybrid S.288 (C. arabica x C. liberica) were used. Separation of PCR products was done by capillary electrophoresis, which allow to discriminate the alleles of each marker. For three markers, specific alleles for either C. arabica or C. liberica species were found. In all cases, S.288 exhibited specific alleles for both species; whereas the INIA-CGC accessions had exclusively C. arabica alleles and EA67 had C. liberica alleles. The BA-48-21O-f marker did not produce PCR fragments for any of the positive controls, suggesting that this marker is not as predictive as the other three to determine the presence of SH3. This work reports the existence of multiple alleles for the Sat244 marker; however, the collection does not have the SH3 mediated-resistance gene. Finally, the utility of capillary electrophoresis as a tool to identify alleles linked to SH3 was demonstrated. |
| first_indexed | 2024-12-20T08:48:53Z |
| format | Article |
| id | doaj.art-8eadda421b9f41e58cdd20719f773157 |
| institution | Directory Open Access Journal |
| issn | 2077-9917 2306-6741 |
| language | English |
| last_indexed | 2024-12-20T08:48:53Z |
| publishDate | 2021-03-01 |
| publisher | Universidad Nacional de Trujillo |
| record_format | Article |
| series | Scientia Agropecuaria |
| spelling | doaj.art-8eadda421b9f41e58cdd20719f7731572022-12-21T19:46:11ZengUniversidad Nacional de TrujilloScientia Agropecuaria2077-99172306-67412021-03-01121Capillary electrophoresis as a tool for genotyping SH3 mediated coffee leaf rust resistanceSavina A. Gutiérrez-Calle0Rosa A. Sánchez-Díaz1Yolanda B. Delgado-Silva2Juan D. Montenegro3Dina L. Gutiérrez4Jorge L. Maicelo-Quintana5Juan C. Guerrero-Abad6Centro Experimental La Molina. Dirección de Recursos Genéticos y Biotecnología. Instituto Nacional de Innovación Agraria (INIA). Av. La Molina 1981, 15024 LimaDepartamento de Fitotecnia. Facultad de Agronomía. Universidad Nacional Agraria La Molina, La Molina. Av. La Molina s/n, 15024 Lima. Centro Experimental La Molina. Dirección de Recursos Genéticos y Biotecnología. Instituto Nacional de Innovación Agraria (INIA). Av. La Molina 1981, 15024 LimaCentro Experimental La Molina. Dirección de Recursos Genéticos y Biotecnología. Instituto Nacional de Innovación Agraria (INIA). Av. La Molina 1981, 15024 Lima.Centro Experimental La Molina. Dirección de Recursos Genéticos y Biotecnología. Instituto Nacional de Innovación Agraria (INIA). Av. La Molina 1981, 15024 LimaCentro Experimental La Molina. Dirección de Recursos Genéticos y Biotecnología. Instituto Nacional de Innovación Agraria (INIA). Av. La Molina 1981, 15024 Lima. Centro Experimental La Molina. Dirección de Recursos Genéticos y Biotecnología. Instituto Nacional de Innovación Agraria (INIA). Av. La Molina 1981, 15024 Lima. Coffee is an important agricultural commodity in the world. However, it is susceptible to Hemileia vastatrix (Hv), an obligatory biotrophic fungus that causes coffee leaf rust (CLR). Natural resistance to rust has been identified in the wild species Coffea canephora and Coffea liberica. These species have been used in breeding programs where interspecific resistant hybrids have been generated. The SH3 gene, derived from C. liberica, has been shown to confer extreme and long-lasting resistance to Hv. A total of 167 accessions of the INIA’s Coffee Germplasm Collection of Peru (INIA-CGC) were screened with 4 markers linked to the SH3 gene. As positive controls, EA67 (C. liberica) and the hybrid S.288 (C. arabica x C. liberica) were used. Separation of PCR products was done by capillary electrophoresis, which allow to discriminate the alleles of each marker. For three markers, specific alleles for either C. arabica or C. liberica species were found. In all cases, S.288 exhibited specific alleles for both species; whereas the INIA-CGC accessions had exclusively C. arabica alleles and EA67 had C. liberica alleles. The BA-48-21O-f marker did not produce PCR fragments for any of the positive controls, suggesting that this marker is not as predictive as the other three to determine the presence of SH3. This work reports the existence of multiple alleles for the Sat244 marker; however, the collection does not have the SH3 mediated-resistance gene. Finally, the utility of capillary electrophoresis as a tool to identify alleles linked to SH3 was demonstrated.http://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/3340coffeecapillaryelectrophoresis;Hemileia vastatrixSH3 |
| spellingShingle | Savina A. Gutiérrez-Calle Rosa A. Sánchez-Díaz Yolanda B. Delgado-Silva Juan D. Montenegro Dina L. Gutiérrez Jorge L. Maicelo-Quintana Juan C. Guerrero-Abad Capillary electrophoresis as a tool for genotyping SH3 mediated coffee leaf rust resistance Scientia Agropecuaria coffee capillary electrophoresis; Hemileia vastatrix SH3 |
| title | Capillary electrophoresis as a tool for genotyping SH3 mediated coffee leaf rust resistance |
| title_full | Capillary electrophoresis as a tool for genotyping SH3 mediated coffee leaf rust resistance |
| title_fullStr | Capillary electrophoresis as a tool for genotyping SH3 mediated coffee leaf rust resistance |
| title_full_unstemmed | Capillary electrophoresis as a tool for genotyping SH3 mediated coffee leaf rust resistance |
| title_short | Capillary electrophoresis as a tool for genotyping SH3 mediated coffee leaf rust resistance |
| title_sort | capillary electrophoresis as a tool for genotyping sh3 mediated coffee leaf rust resistance |
| topic | coffee capillary electrophoresis; Hemileia vastatrix SH3 |
| url | http://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/3340 |
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