Recycling Fish Heads for the Production of Prodigiosin, a Novel Fungicide via Experimental and Molecular Docking Characterization
This report aims to characterize and reuse fish head powder (FHP) as a potential carbon/nitrogen source for microbial fermentation to produce prodigiosin (PG), and to evaluate its novel anti-fungal effect via an experimental and docking study. FHP has high protein (30.25%) and lipid (5.12%) content...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Fishes |
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| Online Access: | https://www.mdpi.com/2410-3888/8/9/468 |
| _version_ | 1797580092945203200 |
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| author | Van Bon Nguyen San-Lang Wang Thi Hanh Nguyen Tu Quy Phan Thi Huyen Nguyen Thi Ha Trang Tran Manh Dung Doan Van Anh Ngo Anh Dzung Nguyen |
| author_facet | Van Bon Nguyen San-Lang Wang Thi Hanh Nguyen Tu Quy Phan Thi Huyen Nguyen Thi Ha Trang Tran Manh Dung Doan Van Anh Ngo Anh Dzung Nguyen |
| author_sort | Van Bon Nguyen |
| collection | DOAJ |
| description | This report aims to characterize and reuse fish head powder (FHP) as a potential carbon/nitrogen source for microbial fermentation to produce prodigiosin (PG), and to evaluate its novel anti-fungal effect via an experimental and docking study. FHP has high protein (30.25%) and lipid (5.12%) content and a diversity of elements, which suggests that FHP is a potential substrate for microbial fermentation. <i>Serratia marcescens</i> TNU01 was screened as the most effective strain to produce PG from FHP. Further experiments indicated that the combination of FHP and crude chitin at the ratio of 9/1 and used at 1.5% as a C/N source for fermentation significantly enhanced PG production. Supplementing a minor amount of 0.05% Ca<sub>3</sub>(PO<sub>4</sub>)<sub>2</sub>, 0.03% K<sub>2</sub>SO<sub>4</sub> also positively affects PG yield production. The fermentation using a bioreactor system (type of 14 L) achieved the highest PG yield production (6.4 mg/mL) in a short cultivation time (10 h) in comparison with the highest PG yield (5.8 mg/mL) obtained after 36 h of fermentation in a flask. In the anti-fungal evaluation of various plant pathogen fungal strains, PG demonstrated efficacy against <i>Fusarium solani</i> F04. The molecular docking results also showed that PG effectively interacted with targeting protein 3QPC of <i>F. solani</i> with good binding energy (DS—9.2 kcal/mol) and an acceptable RMSD value (0.94 Å). In this study, we provide a novel application of FHP for the eco-friendly production of PG, which can potentially used for the management of <i>Fusarium solani</i>. |
| first_indexed | 2024-03-10T22:46:27Z |
| format | Article |
| id | doaj.art-8eafb09aad7a4f73b7b639339da24c83 |
| institution | Directory Open Access Journal |
| issn | 2410-3888 |
| language | English |
| last_indexed | 2024-03-10T22:46:27Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fishes |
| spelling | doaj.art-8eafb09aad7a4f73b7b639339da24c832023-11-19T10:41:07ZengMDPI AGFishes2410-38882023-09-018946810.3390/fishes8090468Recycling Fish Heads for the Production of Prodigiosin, a Novel Fungicide via Experimental and Molecular Docking CharacterizationVan Bon Nguyen0San-Lang Wang1Thi Hanh Nguyen2Tu Quy Phan3Thi Huyen Nguyen4Thi Ha Trang Tran5Manh Dung Doan6Van Anh Ngo7Anh Dzung Nguyen8Institute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot 630000, VietnamDepartment of Chemistry, Tamkang University, New Taipei City 25137, TaiwanDepartment of Chemistry, Tamkang University, New Taipei City 25137, TaiwanDepartment of Science and Technology, Tay Nguyen University, Buon Ma Thuot 630000, VietnamInstitute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot 630000, VietnamInstitute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot 630000, VietnamInstitute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot 630000, VietnamInstitute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot 630000, VietnamInstitute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot 630000, VietnamThis report aims to characterize and reuse fish head powder (FHP) as a potential carbon/nitrogen source for microbial fermentation to produce prodigiosin (PG), and to evaluate its novel anti-fungal effect via an experimental and docking study. FHP has high protein (30.25%) and lipid (5.12%) content and a diversity of elements, which suggests that FHP is a potential substrate for microbial fermentation. <i>Serratia marcescens</i> TNU01 was screened as the most effective strain to produce PG from FHP. Further experiments indicated that the combination of FHP and crude chitin at the ratio of 9/1 and used at 1.5% as a C/N source for fermentation significantly enhanced PG production. Supplementing a minor amount of 0.05% Ca<sub>3</sub>(PO<sub>4</sub>)<sub>2</sub>, 0.03% K<sub>2</sub>SO<sub>4</sub> also positively affects PG yield production. The fermentation using a bioreactor system (type of 14 L) achieved the highest PG yield production (6.4 mg/mL) in a short cultivation time (10 h) in comparison with the highest PG yield (5.8 mg/mL) obtained after 36 h of fermentation in a flask. In the anti-fungal evaluation of various plant pathogen fungal strains, PG demonstrated efficacy against <i>Fusarium solani</i> F04. The molecular docking results also showed that PG effectively interacted with targeting protein 3QPC of <i>F. solani</i> with good binding energy (DS—9.2 kcal/mol) and an acceptable RMSD value (0.94 Å). In this study, we provide a novel application of FHP for the eco-friendly production of PG, which can potentially used for the management of <i>Fusarium solani</i>.https://www.mdpi.com/2410-3888/8/9/468fishery by-productsfish headsmicrobial fermentationprodigiosin<i>Fusarium solani</i>fungicide |
| spellingShingle | Van Bon Nguyen San-Lang Wang Thi Hanh Nguyen Tu Quy Phan Thi Huyen Nguyen Thi Ha Trang Tran Manh Dung Doan Van Anh Ngo Anh Dzung Nguyen Recycling Fish Heads for the Production of Prodigiosin, a Novel Fungicide via Experimental and Molecular Docking Characterization Fishes fishery by-products fish heads microbial fermentation prodigiosin <i>Fusarium solani</i> fungicide |
| title | Recycling Fish Heads for the Production of Prodigiosin, a Novel Fungicide via Experimental and Molecular Docking Characterization |
| title_full | Recycling Fish Heads for the Production of Prodigiosin, a Novel Fungicide via Experimental and Molecular Docking Characterization |
| title_fullStr | Recycling Fish Heads for the Production of Prodigiosin, a Novel Fungicide via Experimental and Molecular Docking Characterization |
| title_full_unstemmed | Recycling Fish Heads for the Production of Prodigiosin, a Novel Fungicide via Experimental and Molecular Docking Characterization |
| title_short | Recycling Fish Heads for the Production of Prodigiosin, a Novel Fungicide via Experimental and Molecular Docking Characterization |
| title_sort | recycling fish heads for the production of prodigiosin a novel fungicide via experimental and molecular docking characterization |
| topic | fishery by-products fish heads microbial fermentation prodigiosin <i>Fusarium solani</i> fungicide |
| url | https://www.mdpi.com/2410-3888/8/9/468 |
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