Modification of the core lipids of low density lipoproteins produces selective alterations in the expression of apoB-100 epitopes.
The conformation of the apolipoprotein B-100 associated with low density lipoproteins (LDL) is not fixed. Rather, the conformations of several regions are subject to alteration by a variety of metabolic and therapeutic perturbations that change either the lipid compositions and/or sizes of LDL parti...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1990-04-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520428391 |
| _version_ | 1818927382786998272 |
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| author | M Kinoshita ES Krul G Schonfeld |
| author_facet | M Kinoshita ES Krul G Schonfeld |
| author_sort | M Kinoshita |
| collection | DOAJ |
| description | The conformation of the apolipoprotein B-100 associated with low density lipoproteins (LDL) is not fixed. Rather, the conformations of several regions are subject to alteration by a variety of metabolic and therapeutic perturbations that change either the lipid compositions and/or sizes of LDL particles. However, because these perturbations simultaneously alter several structural-compositional features of the particles it has been difficult to relate structural-compositional features of LDL to apoB-100 conformations. Furthermore, in in vivo studies several days pass between samplings, thus different sets of particles are studied before and after experimental perturbation. In the present experiments more discrete perturbations of LDL were obtained in vitro by incubating LDL with very low density lipoproteins (VLDL) in the presence of partially purified human plasma lipid transfer proteins. The conformations of apoB on the LDL particles then were examined a) by probing epitope expression and b) by examining interactions between LDL and LDL-receptors in cultured human fibroblasts. During incubations with VLDL and lipid transfer proteins, the diameters of LDL particles decreased; the percentage composition of LDL triglycerides increased three-to fivefold; concomitantly, cholesteryl esters decreased. Lipid transfer protein was required for the transfer to occur and the magnitude of the increase in LDL-triglycerides depended upon the duration of incubation, the ratio of VLDL/LDL, and unknown properties specific to the various LDL preparations. The fact that the triglyceride contents of all LDL preparations were not identically affected suggests that initial packaging of the core region may affect capacity for lipid exchange.(ABSTRACT TRUNCATED AT 250 WORDS) |
| first_indexed | 2024-12-20T03:12:08Z |
| format | Article |
| id | doaj.art-8eb1656c465940a5893634129da24a95 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-20T03:12:08Z |
| publishDate | 1990-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-8eb1656c465940a5893634129da24a952022-12-21T19:55:26ZengElsevierJournal of Lipid Research0022-22751990-04-01314701708Modification of the core lipids of low density lipoproteins produces selective alterations in the expression of apoB-100 epitopes.M Kinoshita0ES Krul1G Schonfeld2Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110.Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110.Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110.The conformation of the apolipoprotein B-100 associated with low density lipoproteins (LDL) is not fixed. Rather, the conformations of several regions are subject to alteration by a variety of metabolic and therapeutic perturbations that change either the lipid compositions and/or sizes of LDL particles. However, because these perturbations simultaneously alter several structural-compositional features of the particles it has been difficult to relate structural-compositional features of LDL to apoB-100 conformations. Furthermore, in in vivo studies several days pass between samplings, thus different sets of particles are studied before and after experimental perturbation. In the present experiments more discrete perturbations of LDL were obtained in vitro by incubating LDL with very low density lipoproteins (VLDL) in the presence of partially purified human plasma lipid transfer proteins. The conformations of apoB on the LDL particles then were examined a) by probing epitope expression and b) by examining interactions between LDL and LDL-receptors in cultured human fibroblasts. During incubations with VLDL and lipid transfer proteins, the diameters of LDL particles decreased; the percentage composition of LDL triglycerides increased three-to fivefold; concomitantly, cholesteryl esters decreased. Lipid transfer protein was required for the transfer to occur and the magnitude of the increase in LDL-triglycerides depended upon the duration of incubation, the ratio of VLDL/LDL, and unknown properties specific to the various LDL preparations. The fact that the triglyceride contents of all LDL preparations were not identically affected suggests that initial packaging of the core region may affect capacity for lipid exchange.(ABSTRACT TRUNCATED AT 250 WORDS)http://www.sciencedirect.com/science/article/pii/S0022227520428391 |
| spellingShingle | M Kinoshita ES Krul G Schonfeld Modification of the core lipids of low density lipoproteins produces selective alterations in the expression of apoB-100 epitopes. Journal of Lipid Research |
| title | Modification of the core lipids of low density lipoproteins produces selective alterations in the expression of apoB-100 epitopes. |
| title_full | Modification of the core lipids of low density lipoproteins produces selective alterations in the expression of apoB-100 epitopes. |
| title_fullStr | Modification of the core lipids of low density lipoproteins produces selective alterations in the expression of apoB-100 epitopes. |
| title_full_unstemmed | Modification of the core lipids of low density lipoproteins produces selective alterations in the expression of apoB-100 epitopes. |
| title_short | Modification of the core lipids of low density lipoproteins produces selective alterations in the expression of apoB-100 epitopes. |
| title_sort | modification of the core lipids of low density lipoproteins produces selective alterations in the expression of apob 100 epitopes |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520428391 |
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