Physiological Mechanism of Exogenous 5-Aminolevulinic Acid Improved the Tolerance of Chinese Cabbage (Brassica pekinensis L.) to Cadmium Stress
The 5-aminolevulinic acid (ALA), a new type of plant growth regulator, can relieve the toxicity of cadmium (Cd) to plants. However, its mechanism has not been thoroughly studied. In the study, the roles of ALA have been investigated in the tolerance of Chinese cabbage (Brassica pekinensis L.) seedli...
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.845396/full |
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| author | Lijing Yang Yue Wu Xiaomin Wang Jian Lv Zhongqi Tang Linli Hu Shilei Luo Ruidong Wang Basharat Ali Jihua Yu Jihua Yu |
| author_facet | Lijing Yang Yue Wu Xiaomin Wang Jian Lv Zhongqi Tang Linli Hu Shilei Luo Ruidong Wang Basharat Ali Jihua Yu Jihua Yu |
| author_sort | Lijing Yang |
| collection | DOAJ |
| description | The 5-aminolevulinic acid (ALA), a new type of plant growth regulator, can relieve the toxicity of cadmium (Cd) to plants. However, its mechanism has not been thoroughly studied. In the study, the roles of ALA have been investigated in the tolerance of Chinese cabbage (Brassica pekinensis L.) seedlings to Cd stress. The results showed that Cd significantly reduced the biomass and the length of the primary root of seedlings but increased the malondialdehyde (MDA) and the hydrogen peroxide (H2O2) contents. These can be effectively mitigated through the application of ALA. The ALA can further induce the activities of antioxidant enzymes in the ascorbate-glutathione (AsA-GSH) cycle under Cd stress, which resulted in high levels of both GSH and AsA. Under ALA + Cd treatment, the seedlings showed a higher chlorophyll content and photosynthetic performance in comparison with Cd treatment alone. Microscopic analysis results confirmed that ALA can protect the cell structure of shoots and roots, i.e., stabilizing the morphological structure of chloroplasts in leaf mesophyll cells. The qRT-PCR results further reported that ALA downregulated the expressions of Cd absorption and transport-related genes in shoots (HMA2 and HMA4) and roots (IRT1, IRT2, Nramp1, and Nramp3), which resulted in the low Cd content in the shoots and roots of cabbage seedlings. Taken together, the exogenous application of ALA alleviates Cd stress through maintaining redox homeostasis, protecting the photosynthetic system, and regulating the expression of Cd transport-related genes in Chinese cabbage seedlings. |
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| language | English |
| last_indexed | 2024-04-12T13:03:40Z |
| publishDate | 2022-05-01 |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-8eb33004515c49c8bfbc23ff27bcb6362022-12-22T03:32:06ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-05-011310.3389/fpls.2022.845396845396Physiological Mechanism of Exogenous 5-Aminolevulinic Acid Improved the Tolerance of Chinese Cabbage (Brassica pekinensis L.) to Cadmium StressLijing Yang0Yue Wu1Xiaomin Wang2Jian Lv3Zhongqi Tang4Linli Hu5Shilei Luo6Ruidong Wang7Basharat Ali8Jihua Yu9Jihua Yu10College of Horticulture, Gansu Agricultural University, Lanzhou, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou, ChinaKey Laboratory of Cell Activities and Stress Adaptations, Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou, ChinaDepartment of Agronomy, University of Agriculture Faisalabad, Faisalabad, PakistanCollege of Horticulture, Gansu Agricultural University, Lanzhou, ChinaGansu Provincial Key Laboratory of Arid Land Crop Science, Gansu Agricultural University, Lanzhou, ChinaThe 5-aminolevulinic acid (ALA), a new type of plant growth regulator, can relieve the toxicity of cadmium (Cd) to plants. However, its mechanism has not been thoroughly studied. In the study, the roles of ALA have been investigated in the tolerance of Chinese cabbage (Brassica pekinensis L.) seedlings to Cd stress. The results showed that Cd significantly reduced the biomass and the length of the primary root of seedlings but increased the malondialdehyde (MDA) and the hydrogen peroxide (H2O2) contents. These can be effectively mitigated through the application of ALA. The ALA can further induce the activities of antioxidant enzymes in the ascorbate-glutathione (AsA-GSH) cycle under Cd stress, which resulted in high levels of both GSH and AsA. Under ALA + Cd treatment, the seedlings showed a higher chlorophyll content and photosynthetic performance in comparison with Cd treatment alone. Microscopic analysis results confirmed that ALA can protect the cell structure of shoots and roots, i.e., stabilizing the morphological structure of chloroplasts in leaf mesophyll cells. The qRT-PCR results further reported that ALA downregulated the expressions of Cd absorption and transport-related genes in shoots (HMA2 and HMA4) and roots (IRT1, IRT2, Nramp1, and Nramp3), which resulted in the low Cd content in the shoots and roots of cabbage seedlings. Taken together, the exogenous application of ALA alleviates Cd stress through maintaining redox homeostasis, protecting the photosynthetic system, and regulating the expression of Cd transport-related genes in Chinese cabbage seedlings.https://www.frontiersin.org/articles/10.3389/fpls.2022.845396/full5-minolevulinic acidcadmium stressChinese cabbageascorbate-lutathione cyclephotosy nthesis |
| spellingShingle | Lijing Yang Yue Wu Xiaomin Wang Jian Lv Zhongqi Tang Linli Hu Shilei Luo Ruidong Wang Basharat Ali Jihua Yu Jihua Yu Physiological Mechanism of Exogenous 5-Aminolevulinic Acid Improved the Tolerance of Chinese Cabbage (Brassica pekinensis L.) to Cadmium Stress Frontiers in Plant Science 5-minolevulinic acid cadmium stress Chinese cabbage ascorbate-lutathione cycle photosy nthesis |
| title | Physiological Mechanism of Exogenous 5-Aminolevulinic Acid Improved the Tolerance of Chinese Cabbage (Brassica pekinensis L.) to Cadmium Stress |
| title_full | Physiological Mechanism of Exogenous 5-Aminolevulinic Acid Improved the Tolerance of Chinese Cabbage (Brassica pekinensis L.) to Cadmium Stress |
| title_fullStr | Physiological Mechanism of Exogenous 5-Aminolevulinic Acid Improved the Tolerance of Chinese Cabbage (Brassica pekinensis L.) to Cadmium Stress |
| title_full_unstemmed | Physiological Mechanism of Exogenous 5-Aminolevulinic Acid Improved the Tolerance of Chinese Cabbage (Brassica pekinensis L.) to Cadmium Stress |
| title_short | Physiological Mechanism of Exogenous 5-Aminolevulinic Acid Improved the Tolerance of Chinese Cabbage (Brassica pekinensis L.) to Cadmium Stress |
| title_sort | physiological mechanism of exogenous 5 aminolevulinic acid improved the tolerance of chinese cabbage brassica pekinensis l to cadmium stress |
| topic | 5-minolevulinic acid cadmium stress Chinese cabbage ascorbate-lutathione cycle photosy nthesis |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.845396/full |
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