Effects of KCN, SHAM and Oxygen Concentrations on Respiratory Properties of Purified Mitochondria Isolated from Ananas comosus (Pineapple) and Kalanchoë daigremontiana
Effects of potassium cyanide (KCN), salicylhydroxamic acid (SHAM), and oxygen concentrations on mitochondrial respiration were investigated in purified mitochondria of a typical phosphoenolpyruvate carboxykinase (PCK) crassulacean acid metabolism (CAM) plant Ananas comosus (pineapple) and a typical...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2005-01-01
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| Series: | Plant Production Science |
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| Online Access: | http://dx.doi.org/10.1626/pps.8.383 |
| _version_ | 1818962873365299200 |
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| author | Hoang Thi Kim Hong Akihiro Nose Sakae Agarie |
| author_facet | Hoang Thi Kim Hong Akihiro Nose Sakae Agarie |
| author_sort | Hoang Thi Kim Hong |
| collection | DOAJ |
| description | Effects of potassium cyanide (KCN), salicylhydroxamic acid (SHAM), and oxygen concentrations on mitochondrial respiration were investigated in purified mitochondria of a typical phosphoenolpyruvate carboxykinase (PCK) crassulacean acid metabolism (CAM) plant Ananas comosus (pineapple) and a typical malic enzyme (ME)-CAM plant Kalanchoë daigremontiana. Mitochondria of A. comosus oxidized succinate and NADH faster than that of K. daigremontiana. Succinate and NADH oxidations in mitochondria of both species were partially inhibited by KCN and SHAM, indicating that these oxidations were connected to cytochrome and alternative pathways in their electron transport chain (ETC). NADH oxidation was more sensitive to KCN than succinate oxidation, suggesting that the ETC from NADH oxidation was less connected to the alternative pathway than that from succinate in mitochondria of both species. Concurrent oxidation of succinate and NADH resulted in much higher rates of cytochrome and alternative respirations than each individual oxidation alone in both species. NADH oxidation in A. comosus mitochondria was more connected to the cytochrome pathway, so A. comosus could produce much more ATP than K. daigremontiana. This capacity might be one of the fitting mechanisms of A. comosus to produce a sufficient amount of ATP for cytosolic PCK in the daytime. In addition, the reduction of oxygen concentrations decreased not only the cytochrome respiration, but also the alternative respiration on succinate oxidation in mitochondria of both species, and the decrease was greater in K. daigremontiana than in A. comosus. |
| first_indexed | 2024-12-20T12:36:14Z |
| format | Article |
| id | doaj.art-8eb44c2c917d4b1ca0ef817ae232975f |
| institution | Directory Open Access Journal |
| issn | 1343-943X 1349-1008 |
| language | English |
| last_indexed | 2024-12-20T12:36:14Z |
| publishDate | 2005-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Plant Production Science |
| spelling | doaj.art-8eb44c2c917d4b1ca0ef817ae232975f2022-12-21T19:40:36ZengTaylor & Francis GroupPlant Production Science1343-943X1349-10082005-01-018438339210.1626/pps.8.38311644713Effects of KCN, SHAM and Oxygen Concentrations on Respiratory Properties of Purified Mitochondria Isolated from Ananas comosus (Pineapple) and Kalanchoë daigremontianaHoang Thi Kim Hong0Akihiro Nose1Sakae Agarie2Kagoshima UniversitySaga UniversitySaga UniversityEffects of potassium cyanide (KCN), salicylhydroxamic acid (SHAM), and oxygen concentrations on mitochondrial respiration were investigated in purified mitochondria of a typical phosphoenolpyruvate carboxykinase (PCK) crassulacean acid metabolism (CAM) plant Ananas comosus (pineapple) and a typical malic enzyme (ME)-CAM plant Kalanchoë daigremontiana. Mitochondria of A. comosus oxidized succinate and NADH faster than that of K. daigremontiana. Succinate and NADH oxidations in mitochondria of both species were partially inhibited by KCN and SHAM, indicating that these oxidations were connected to cytochrome and alternative pathways in their electron transport chain (ETC). NADH oxidation was more sensitive to KCN than succinate oxidation, suggesting that the ETC from NADH oxidation was less connected to the alternative pathway than that from succinate in mitochondria of both species. Concurrent oxidation of succinate and NADH resulted in much higher rates of cytochrome and alternative respirations than each individual oxidation alone in both species. NADH oxidation in A. comosus mitochondria was more connected to the cytochrome pathway, so A. comosus could produce much more ATP than K. daigremontiana. This capacity might be one of the fitting mechanisms of A. comosus to produce a sufficient amount of ATP for cytosolic PCK in the daytime. In addition, the reduction of oxygen concentrations decreased not only the cytochrome respiration, but also the alternative respiration on succinate oxidation in mitochondria of both species, and the decrease was greater in K. daigremontiana than in A. comosus.http://dx.doi.org/10.1626/pps.8.383Alternative pathwayAnanas comosusCytochrome pathwayKalanchoë daigremontianaMitochondriaOxygen |
| spellingShingle | Hoang Thi Kim Hong Akihiro Nose Sakae Agarie Effects of KCN, SHAM and Oxygen Concentrations on Respiratory Properties of Purified Mitochondria Isolated from Ananas comosus (Pineapple) and Kalanchoë daigremontiana Plant Production Science Alternative pathway Ananas comosus Cytochrome pathway Kalanchoë daigremontiana Mitochondria Oxygen |
| title | Effects of KCN, SHAM and Oxygen Concentrations on Respiratory Properties of Purified Mitochondria Isolated from Ananas comosus (Pineapple) and Kalanchoë daigremontiana |
| title_full | Effects of KCN, SHAM and Oxygen Concentrations on Respiratory Properties of Purified Mitochondria Isolated from Ananas comosus (Pineapple) and Kalanchoë daigremontiana |
| title_fullStr | Effects of KCN, SHAM and Oxygen Concentrations on Respiratory Properties of Purified Mitochondria Isolated from Ananas comosus (Pineapple) and Kalanchoë daigremontiana |
| title_full_unstemmed | Effects of KCN, SHAM and Oxygen Concentrations on Respiratory Properties of Purified Mitochondria Isolated from Ananas comosus (Pineapple) and Kalanchoë daigremontiana |
| title_short | Effects of KCN, SHAM and Oxygen Concentrations on Respiratory Properties of Purified Mitochondria Isolated from Ananas comosus (Pineapple) and Kalanchoë daigremontiana |
| title_sort | effects of kcn sham and oxygen concentrations on respiratory properties of purified mitochondria isolated from ananas comosus pineapple and kalanchoe daigremontiana |
| topic | Alternative pathway Ananas comosus Cytochrome pathway Kalanchoë daigremontiana Mitochondria Oxygen |
| url | http://dx.doi.org/10.1626/pps.8.383 |
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