MALAT1/ mir-1-3p mediated BRF2 expression promotes HCC progression via inhibiting the LKB1/AMPK signaling pathway
Abstract Background The long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) has been reported to play a vital role in the occurrence and development of various tumors. However, the underlying mechanism of MALAT1 in hepatocellular carcinoma (HCC) has not been thoroughl...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-08-01
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| Series: | Cancer Cell International |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12935-023-03034-1 |
| _version_ | 1827175715119824896 |
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| author | Guang-Zhen Li Guang-Xiao Meng Guo-Qiang Pan Xiao Zhang Lun-Jie Yan Rui-Zhe Li Zi-Niu Ding Si-Yu Tan Dong-Xu Wang Bao-wen Tian Yu-Chuan Yan Zhao-Ru Dong Jian-Guo Hong Tao Li |
| author_facet | Guang-Zhen Li Guang-Xiao Meng Guo-Qiang Pan Xiao Zhang Lun-Jie Yan Rui-Zhe Li Zi-Niu Ding Si-Yu Tan Dong-Xu Wang Bao-wen Tian Yu-Chuan Yan Zhao-Ru Dong Jian-Guo Hong Tao Li |
| author_sort | Guang-Zhen Li |
| collection | DOAJ |
| description | Abstract Background The long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) has been reported to play a vital role in the occurrence and development of various tumors. However, the underlying mechanism of MALAT1 in hepatocellular carcinoma (HCC) has not been thoroughly elucidated. Methods The expression levels of MALAT1 in HCC tissues and different cell lines were detected by qRT-PCR. Antisense oligonucleotides (ASO)-MALAT1 transfected cells were used to explore the biological effects of MALAT1 in HCC cells by cell counting kit 8 (CCK-8), colony formation, transwell, wound healing, and flow cytometry analysis. Western blotting was performed to measure AMPK and apoptosis-related protein levels. Dual-luciferase reporter assay was performed to verify the relationship between MALAT1 and its specific targets. Results We found that MALAT1 was upregulated in HCC, and MALAT1 knockdown in HCC cells inhibited cell proliferation, migration, and invasion and inhibited apoptosis in vitro. Further studies demonstrated that MALAT1 positively regulated the expression of transcription factor II B‑related factor 2 (BRF2), which was associated with tumor recurrence, large tumor size, and poor prognosis in HCC. Mechanistically, MALAT1 was found to act as a competitive endogenous RNA to sponge has-miR-1-3p, which upregulated BRF2 expression. Knockdown of BRF2 inhibited the progression of HCC by activating the LKB1/AMPK signaling pathway. Overexpression of BRF2 reversed the inhibitory effect of MALAT1 knockdown on HCC cell viability. Moreover, ASO targeting MALAT1 inhibited the growth of xenograft tumors. Conclusions Our results demonstrate a novel MALAT1/miR-1-3p/BRF2/LKB1/AMPK regulatory axis in HCC, which may provide new molecular therapeutic targets for HCC in the future. |
| first_indexed | 2024-03-10T17:00:01Z |
| format | Article |
| id | doaj.art-8eb66f216b24440f9fb096fca92b6f63 |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2025-03-21T04:15:51Z |
| publishDate | 2023-08-01 |
| publisher | BMC |
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| series | Cancer Cell International |
| spelling | doaj.art-8eb66f216b24440f9fb096fca92b6f632024-07-28T11:33:21ZengBMCCancer Cell International1475-28672023-08-0123111610.1186/s12935-023-03034-1MALAT1/ mir-1-3p mediated BRF2 expression promotes HCC progression via inhibiting the LKB1/AMPK signaling pathwayGuang-Zhen Li0Guang-Xiao Meng1Guo-Qiang Pan2Xiao Zhang3Lun-Jie Yan4Rui-Zhe Li5Zi-Niu Ding6Si-Yu Tan7Dong-Xu Wang8Bao-wen Tian9Yu-Chuan Yan10Zhao-Ru Dong11Jian-Guo Hong12Tao Li13Medical Integration and Practice Center, Cheeloo College of Medicine, Shandong UniversityDepartment of General Surgery, Qilu Hospital of Shandong UniversityDepartment of General Surgery, Qilu Hospital of Shandong UniversityDepartment of General Surgery, Qilu Hospital of Shandong UniversityDepartment of General Surgery, Qilu Hospital of Shandong UniversityDepartment of General Surgery, Qilu Hospital of Shandong UniversityDepartment of General Surgery, Qilu Hospital of Shandong UniversityDepartment of General Surgery, Qilu Hospital of Shandong UniversityDepartment of General Surgery, Qilu Hospital of Shandong UniversityDepartment of General Surgery, Qilu Hospital of Shandong UniversityDepartment of General Surgery, Qilu Hospital of Shandong UniversityDepartment of General Surgery, Qilu Hospital of Shandong UniversityDepartment of General Surgery, Qilu Hospital of Shandong UniversityDepartment of General Surgery, Qilu Hospital of Shandong UniversityAbstract Background The long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) has been reported to play a vital role in the occurrence and development of various tumors. However, the underlying mechanism of MALAT1 in hepatocellular carcinoma (HCC) has not been thoroughly elucidated. Methods The expression levels of MALAT1 in HCC tissues and different cell lines were detected by qRT-PCR. Antisense oligonucleotides (ASO)-MALAT1 transfected cells were used to explore the biological effects of MALAT1 in HCC cells by cell counting kit 8 (CCK-8), colony formation, transwell, wound healing, and flow cytometry analysis. Western blotting was performed to measure AMPK and apoptosis-related protein levels. Dual-luciferase reporter assay was performed to verify the relationship between MALAT1 and its specific targets. Results We found that MALAT1 was upregulated in HCC, and MALAT1 knockdown in HCC cells inhibited cell proliferation, migration, and invasion and inhibited apoptosis in vitro. Further studies demonstrated that MALAT1 positively regulated the expression of transcription factor II B‑related factor 2 (BRF2), which was associated with tumor recurrence, large tumor size, and poor prognosis in HCC. Mechanistically, MALAT1 was found to act as a competitive endogenous RNA to sponge has-miR-1-3p, which upregulated BRF2 expression. Knockdown of BRF2 inhibited the progression of HCC by activating the LKB1/AMPK signaling pathway. Overexpression of BRF2 reversed the inhibitory effect of MALAT1 knockdown on HCC cell viability. Moreover, ASO targeting MALAT1 inhibited the growth of xenograft tumors. Conclusions Our results demonstrate a novel MALAT1/miR-1-3p/BRF2/LKB1/AMPK regulatory axis in HCC, which may provide new molecular therapeutic targets for HCC in the future.https://doi.org/10.1186/s12935-023-03034-1Hepatocellular carcinomaBRF2MALAT1Has-miR-1-3p |
| spellingShingle | Guang-Zhen Li Guang-Xiao Meng Guo-Qiang Pan Xiao Zhang Lun-Jie Yan Rui-Zhe Li Zi-Niu Ding Si-Yu Tan Dong-Xu Wang Bao-wen Tian Yu-Chuan Yan Zhao-Ru Dong Jian-Guo Hong Tao Li MALAT1/ mir-1-3p mediated BRF2 expression promotes HCC progression via inhibiting the LKB1/AMPK signaling pathway Cancer Cell International Hepatocellular carcinoma BRF2 MALAT1 Has-miR-1-3p |
| title | MALAT1/ mir-1-3p mediated BRF2 expression promotes HCC progression via inhibiting the LKB1/AMPK signaling pathway |
| title_full | MALAT1/ mir-1-3p mediated BRF2 expression promotes HCC progression via inhibiting the LKB1/AMPK signaling pathway |
| title_fullStr | MALAT1/ mir-1-3p mediated BRF2 expression promotes HCC progression via inhibiting the LKB1/AMPK signaling pathway |
| title_full_unstemmed | MALAT1/ mir-1-3p mediated BRF2 expression promotes HCC progression via inhibiting the LKB1/AMPK signaling pathway |
| title_short | MALAT1/ mir-1-3p mediated BRF2 expression promotes HCC progression via inhibiting the LKB1/AMPK signaling pathway |
| title_sort | malat1 mir 1 3p mediated brf2 expression promotes hcc progression via inhibiting the lkb1 ampk signaling pathway |
| topic | Hepatocellular carcinoma BRF2 MALAT1 Has-miR-1-3p |
| url | https://doi.org/10.1186/s12935-023-03034-1 |
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