Visualization of bone formation in sheep’s middle ear by using fluorochrome sequential labelling (FSL)
Abstract One factor for the lacking integration of the middle ear stapes footplate prosthesis or the missing healing of stapes footplate fractures could be the known osteogenic inactivity. In contrast, it was recently demonstrated that titanium prostheses with an applied collagen matrix and immobili...
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Nature Portfolio
2024-03-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-57630-w |
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| author | Max Kemper Anne Kluge Michael Ney Thomas Beleites Ines Zeidler-Rentzsch Christiane Keil Thomas Zahnert Marcus Neudert |
| author_facet | Max Kemper Anne Kluge Michael Ney Thomas Beleites Ines Zeidler-Rentzsch Christiane Keil Thomas Zahnert Marcus Neudert |
| author_sort | Max Kemper |
| collection | DOAJ |
| description | Abstract One factor for the lacking integration of the middle ear stapes footplate prosthesis or the missing healing of stapes footplate fractures could be the known osteogenic inactivity. In contrast, it was recently demonstrated that titanium prostheses with an applied collagen matrix and immobilised growth factors stimulate osteoblastic activation and differentiation on the stapes footplate. Regarding those findings, the aim of this study was to evaluate the potential of bone regeneration including bone remodeling in the middle ear. Ten one-year-old female merino sheep underwent a middle ear surgery without implantation of middle ear prostheses or any other component for activating bone formation. Post-operatively, four fluorochromes (tetracycline, alizarin complexion, calcein green and xylenol orange) were administered by subcutaneous injection at different time points after surgery (1 day: tetracycline, 7 days: alizarin, 14 days: calcein, 28 days: xylenol). After 12 weeks, the temporal bones including the lateral skull base were extracted and histologically analyzed. Fluorescence microscopy analysis of the entire stapes with the oval niche, but in particular stapes footplate and the Crura stapedis revealed evidence of new bone formation. Calcein was detected in all and xylenol in 60% of the animals. In contrast, tetracycline and alizarin could only be verified in two animals. The authors were able to demonstrate the osseoregenerative potential of the middle ear, in particular of the stapes footplate, using fluorescence sequence labelling. |
| first_indexed | 2024-04-24T16:18:49Z |
| format | Article |
| id | doaj.art-8eb8024da7944ef6afa2e81427297eb4 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T16:18:49Z |
| publishDate | 2024-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-8eb8024da7944ef6afa2e81427297eb42024-03-31T11:17:51ZengNature PortfolioScientific Reports2045-23222024-03-011411710.1038/s41598-024-57630-wVisualization of bone formation in sheep’s middle ear by using fluorochrome sequential labelling (FSL)Max Kemper0Anne Kluge1Michael Ney2Thomas Beleites3Ines Zeidler-Rentzsch4Christiane Keil5Thomas Zahnert6Marcus Neudert7Department of Otorhinolaryngology Head and Neck Surgery, Faculty of Medicine (and University Hospital) Carl Gustav Carus, Technische Universität DresdenDepartment of Otorhinolaryngology Head and Neck Surgery, Faculty of Medicine (and University Hospital) Carl Gustav Carus, Technische Universität DresdenDepartment of Audiology and Phoniatrics, Charité – University Medicine BerlinDepartment of Otorhinolaryngology Head and Neck Surgery, Faculty of Medicine (and University Hospital) Carl Gustav Carus, Technische Universität DresdenDepartment of Otorhinolaryngology Head and Neck Surgery, Faculty of Medicine (and University Hospital) Carl Gustav Carus, Technische Universität DresdenDepartment of Orthodontics, Faculty of Medicine (and University Hospital) Carl Gustav Carus, Technische Universität DresdenDepartment of Otorhinolaryngology Head and Neck Surgery, Faculty of Medicine (and University Hospital) Carl Gustav Carus, Technische Universität DresdenDepartment of Otorhinolaryngology Head and Neck Surgery, Faculty of Medicine (and University Hospital) Carl Gustav Carus, Technische Universität DresdenAbstract One factor for the lacking integration of the middle ear stapes footplate prosthesis or the missing healing of stapes footplate fractures could be the known osteogenic inactivity. In contrast, it was recently demonstrated that titanium prostheses with an applied collagen matrix and immobilised growth factors stimulate osteoblastic activation and differentiation on the stapes footplate. Regarding those findings, the aim of this study was to evaluate the potential of bone regeneration including bone remodeling in the middle ear. Ten one-year-old female merino sheep underwent a middle ear surgery without implantation of middle ear prostheses or any other component for activating bone formation. Post-operatively, four fluorochromes (tetracycline, alizarin complexion, calcein green and xylenol orange) were administered by subcutaneous injection at different time points after surgery (1 day: tetracycline, 7 days: alizarin, 14 days: calcein, 28 days: xylenol). After 12 weeks, the temporal bones including the lateral skull base were extracted and histologically analyzed. Fluorescence microscopy analysis of the entire stapes with the oval niche, but in particular stapes footplate and the Crura stapedis revealed evidence of new bone formation. Calcein was detected in all and xylenol in 60% of the animals. In contrast, tetracycline and alizarin could only be verified in two animals. The authors were able to demonstrate the osseoregenerative potential of the middle ear, in particular of the stapes footplate, using fluorescence sequence labelling.https://doi.org/10.1038/s41598-024-57630-wMammalian middle earBone formationOsseoregenerative processStapes footplateFluorochrome sequence labelling |
| spellingShingle | Max Kemper Anne Kluge Michael Ney Thomas Beleites Ines Zeidler-Rentzsch Christiane Keil Thomas Zahnert Marcus Neudert Visualization of bone formation in sheep’s middle ear by using fluorochrome sequential labelling (FSL) Scientific Reports Mammalian middle ear Bone formation Osseoregenerative process Stapes footplate Fluorochrome sequence labelling |
| title | Visualization of bone formation in sheep’s middle ear by using fluorochrome sequential labelling (FSL) |
| title_full | Visualization of bone formation in sheep’s middle ear by using fluorochrome sequential labelling (FSL) |
| title_fullStr | Visualization of bone formation in sheep’s middle ear by using fluorochrome sequential labelling (FSL) |
| title_full_unstemmed | Visualization of bone formation in sheep’s middle ear by using fluorochrome sequential labelling (FSL) |
| title_short | Visualization of bone formation in sheep’s middle ear by using fluorochrome sequential labelling (FSL) |
| title_sort | visualization of bone formation in sheep s middle ear by using fluorochrome sequential labelling fsl |
| topic | Mammalian middle ear Bone formation Osseoregenerative process Stapes footplate Fluorochrome sequence labelling |
| url | https://doi.org/10.1038/s41598-024-57630-w |
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