Molecular characterisation of the oldest domesticated Turkish einkorn wheat landraces with simple sequence repeat (SSR) markers
Einkorn (Triticum monococcum L. ssp. monococcum) is an ancient diploid wheat species with many useful traits and used as a wheat gene discovery model. In this research, a total of 41 diploid and tetraploid wheat accessions were tested using simple sequence repeat (SSR) markers. A total of 33 genotyp...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2021-01-01
|
| Series: | Biotechnology & Biotechnological Equipment |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/13102818.2021.1970023 |
| _version_ | 1818650792102461440 |
|---|---|
| author | Ayse Yildiz Mortaza Hajyzadeh Kursad Ozbek Muhammad Azhar Nadeem Danny Hunter |
| author_facet | Ayse Yildiz Mortaza Hajyzadeh Kursad Ozbek Muhammad Azhar Nadeem Danny Hunter |
| author_sort | Ayse Yildiz |
| collection | DOAJ |
| description | Einkorn (Triticum monococcum L. ssp. monococcum) is an ancient diploid wheat species with many useful traits and used as a wheat gene discovery model. In this research, a total of 41 diploid and tetraploid wheat accessions were tested using simple sequence repeat (SSR) markers. A total of 33 genotypes of T. monococcum along with four genotypes each of tetraploid wheat (T. dicoccon and T. durum) were used as plant material. The analysis utilized 10 polymorphic markers, including a total number of 41 alleles with an average frequency of 4.1 alleles per locus during exploration of the level of genetic variations. Various diversity analyses, which are the effective number of alleles (Ne), gene diversity (h), Polymorphic Information Content (PIC), and Shannon’s information index (I), were performed for 10 ‘A’ genome wheat SSR markers. The results showed a narrow variation in einkorn genotypes, supported by Analysis of Molecular Variance (AMOVA), with 66% maximum variation in all genotypes. The structure analysis divided the whole germplasm into two populations. A dendrogram was constructed to determine the genetic similarities using the unpaired group method with arithmetic averages (UPGMA), which separated tetraploid wheat from other genotypes/accessions. Principal Coordinate Analysis (PCoA) co-supported the clustering of UPGMA and structure by differentiating the diploid and tetraploid wheat. These findings will help understand the genetic relationships among these wheat accessions and their use in breeding programs in the future works. |
| first_indexed | 2024-12-17T01:55:50Z |
| format | Article |
| id | doaj.art-8ebc088e2d564e3b8b64a39442fa5ea3 |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-12-17T01:55:50Z |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-8ebc088e2d564e3b8b64a39442fa5ea32022-12-21T22:07:58ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302021-01-013511291130010.1080/13102818.2021.19700231970023Molecular characterisation of the oldest domesticated Turkish einkorn wheat landraces with simple sequence repeat (SSR) markersAyse Yildiz0Mortaza Hajyzadeh1Kursad Ozbek2Muhammad Azhar Nadeem3Danny Hunter4The National Botanical Garden of Turkey, Rebublic of Turkey Ministry of Agriculture and Forestry, Üniversiteler Mah. Dumlupınar BoulevardDepartment of Field Crops, Faculty of Agriculture, Şirnak UniversityThe National Botanical Garden of Turkey, Rebublic of Turkey Ministry of Agriculture and Forestry, Üniversiteler Mah. Dumlupınar BoulevardFaculty of Agricultural Sciences and Technologies, Sivas University of Science and TechnologyAlliance of Bioversity International and CIATEinkorn (Triticum monococcum L. ssp. monococcum) is an ancient diploid wheat species with many useful traits and used as a wheat gene discovery model. In this research, a total of 41 diploid and tetraploid wheat accessions were tested using simple sequence repeat (SSR) markers. A total of 33 genotypes of T. monococcum along with four genotypes each of tetraploid wheat (T. dicoccon and T. durum) were used as plant material. The analysis utilized 10 polymorphic markers, including a total number of 41 alleles with an average frequency of 4.1 alleles per locus during exploration of the level of genetic variations. Various diversity analyses, which are the effective number of alleles (Ne), gene diversity (h), Polymorphic Information Content (PIC), and Shannon’s information index (I), were performed for 10 ‘A’ genome wheat SSR markers. The results showed a narrow variation in einkorn genotypes, supported by Analysis of Molecular Variance (AMOVA), with 66% maximum variation in all genotypes. The structure analysis divided the whole germplasm into two populations. A dendrogram was constructed to determine the genetic similarities using the unpaired group method with arithmetic averages (UPGMA), which separated tetraploid wheat from other genotypes/accessions. Principal Coordinate Analysis (PCoA) co-supported the clustering of UPGMA and structure by differentiating the diploid and tetraploid wheat. These findings will help understand the genetic relationships among these wheat accessions and their use in breeding programs in the future works.http://dx.doi.org/10.1080/13102818.2021.1970023 triticum monoccocum genetic diversityssreinkorn wheatpolymorphismgenetic relationships |
| spellingShingle | Ayse Yildiz Mortaza Hajyzadeh Kursad Ozbek Muhammad Azhar Nadeem Danny Hunter Molecular characterisation of the oldest domesticated Turkish einkorn wheat landraces with simple sequence repeat (SSR) markers Biotechnology & Biotechnological Equipment triticum monoccocum genetic diversity ssr einkorn wheat polymorphism genetic relationships |
| title | Molecular characterisation of the oldest domesticated Turkish einkorn wheat landraces with simple sequence repeat (SSR) markers |
| title_full | Molecular characterisation of the oldest domesticated Turkish einkorn wheat landraces with simple sequence repeat (SSR) markers |
| title_fullStr | Molecular characterisation of the oldest domesticated Turkish einkorn wheat landraces with simple sequence repeat (SSR) markers |
| title_full_unstemmed | Molecular characterisation of the oldest domesticated Turkish einkorn wheat landraces with simple sequence repeat (SSR) markers |
| title_short | Molecular characterisation of the oldest domesticated Turkish einkorn wheat landraces with simple sequence repeat (SSR) markers |
| title_sort | molecular characterisation of the oldest domesticated turkish einkorn wheat landraces with simple sequence repeat ssr markers |
| topic | triticum monoccocum genetic diversity ssr einkorn wheat polymorphism genetic relationships |
| url | http://dx.doi.org/10.1080/13102818.2021.1970023 |
| work_keys_str_mv | AT ayseyildiz molecularcharacterisationoftheoldestdomesticatedturkisheinkornwheatlandraceswithsimplesequencerepeatssrmarkers AT mortazahajyzadeh molecularcharacterisationoftheoldestdomesticatedturkisheinkornwheatlandraceswithsimplesequencerepeatssrmarkers AT kursadozbek molecularcharacterisationoftheoldestdomesticatedturkisheinkornwheatlandraceswithsimplesequencerepeatssrmarkers AT muhammadazharnadeem molecularcharacterisationoftheoldestdomesticatedturkisheinkornwheatlandraceswithsimplesequencerepeatssrmarkers AT dannyhunter molecularcharacterisationoftheoldestdomesticatedturkisheinkornwheatlandraceswithsimplesequencerepeatssrmarkers |