Univariate spectrophotometry and multivariate calibration: Stability-indicating analytical tools for the quantification of pimozide in bulk and pharmaceutical dosage form
Simple, accurate sensitive and precise spectrophotometric and chemometric stability indicating techniques were adopted for the determination of Pimozide (PIM) in presence of its alkaline and acidic degradation products over a concentration range of 10–100 μg mL−1. The proposed spectrophotometric tec...
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| Format: | Article |
| Language: | English |
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Faculty of Pharmacy, Cairo University
2015-12-01
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| Series: | Bulletin of Faculty of Pharmacy Cairo University |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1110093115000307 |
| _version_ | 1797969762882420736 |
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| author | Amal Mahmoud Abou Al Alamein Lobna Abd El Aziz Hussien Ekram Hany Mohamed |
| author_facet | Amal Mahmoud Abou Al Alamein Lobna Abd El Aziz Hussien Ekram Hany Mohamed |
| author_sort | Amal Mahmoud Abou Al Alamein |
| collection | DOAJ |
| description | Simple, accurate sensitive and precise spectrophotometric and chemometric stability indicating techniques were adopted for the determination of Pimozide (PIM) in presence of its alkaline and acidic degradation products over a concentration range of 10–100 μg mL−1. The proposed spectrophotometric technique includes first derivative (D1) spectrophotometric one at 252 nm and 256.6 nm in presence of its acidic and alkaline degradates, respectively, first-derivative of the ratio spectra spectrophotometry (DR1) at 292.5 nm, the Q-analysis (absorption ratio) method, which involves the formation of absorbance equation at 242.2 nm and 281.7 nm, dual wave length method at 270.1 nm and 284 nm, the H-point standard addition method (HPSAM) and the mean centering of the ratio spectra method. The second technique is chemometric methods which include determination of PIM in presence of both its acidic and alkaline degradates using multivariate calibration methods [the classical least squares (CLS), principle component regression (PCR) and partial least squares (PLS)] using the information contained in the absorption spectra. The proposed methods have been successfully applied to the analysis of PIM in pharmaceutical dosage forms without interference from other dosage form additives and the results were statistically compared with the official method. |
| first_indexed | 2024-04-11T03:07:23Z |
| format | Article |
| id | doaj.art-8ebc793653924c3db3202b66f678400b |
| institution | Directory Open Access Journal |
| issn | 1110-0931 |
| language | English |
| last_indexed | 2024-04-11T03:07:23Z |
| publishDate | 2015-12-01 |
| publisher | Faculty of Pharmacy, Cairo University |
| record_format | Article |
| series | Bulletin of Faculty of Pharmacy Cairo University |
| spelling | doaj.art-8ebc793653924c3db3202b66f678400b2023-01-02T12:52:18ZengFaculty of Pharmacy, Cairo UniversityBulletin of Faculty of Pharmacy Cairo University1110-09312015-12-0153217318310.1016/j.bfopcu.2015.10.003Univariate spectrophotometry and multivariate calibration: Stability-indicating analytical tools for the quantification of pimozide in bulk and pharmaceutical dosage formAmal Mahmoud Abou Al Alamein0Lobna Abd El Aziz Hussien1Ekram Hany Mohamed2Cairo University, Faculty of Pharmacy, Analytical Chemistry Department, Kasr el Aini St, Cairo, EgyptAin Shams University, Faculty of Pharmacy, Analytical Chemistry Department, Abbassia, Cairo, EgyptModern Sciences and Arts University Analytical Chemistry Department, 6th of October City, EgyptSimple, accurate sensitive and precise spectrophotometric and chemometric stability indicating techniques were adopted for the determination of Pimozide (PIM) in presence of its alkaline and acidic degradation products over a concentration range of 10–100 μg mL−1. The proposed spectrophotometric technique includes first derivative (D1) spectrophotometric one at 252 nm and 256.6 nm in presence of its acidic and alkaline degradates, respectively, first-derivative of the ratio spectra spectrophotometry (DR1) at 292.5 nm, the Q-analysis (absorption ratio) method, which involves the formation of absorbance equation at 242.2 nm and 281.7 nm, dual wave length method at 270.1 nm and 284 nm, the H-point standard addition method (HPSAM) and the mean centering of the ratio spectra method. The second technique is chemometric methods which include determination of PIM in presence of both its acidic and alkaline degradates using multivariate calibration methods [the classical least squares (CLS), principle component regression (PCR) and partial least squares (PLS)] using the information contained in the absorption spectra. The proposed methods have been successfully applied to the analysis of PIM in pharmaceutical dosage forms without interference from other dosage form additives and the results were statistically compared with the official method.http://www.sciencedirect.com/science/article/pii/S1110093115000307PimozideStabilitySpectrophotometryChemometric methods |
| spellingShingle | Amal Mahmoud Abou Al Alamein Lobna Abd El Aziz Hussien Ekram Hany Mohamed Univariate spectrophotometry and multivariate calibration: Stability-indicating analytical tools for the quantification of pimozide in bulk and pharmaceutical dosage form Bulletin of Faculty of Pharmacy Cairo University Pimozide Stability Spectrophotometry Chemometric methods |
| title | Univariate spectrophotometry and multivariate calibration: Stability-indicating analytical tools for the quantification of pimozide in bulk and pharmaceutical dosage form |
| title_full | Univariate spectrophotometry and multivariate calibration: Stability-indicating analytical tools for the quantification of pimozide in bulk and pharmaceutical dosage form |
| title_fullStr | Univariate spectrophotometry and multivariate calibration: Stability-indicating analytical tools for the quantification of pimozide in bulk and pharmaceutical dosage form |
| title_full_unstemmed | Univariate spectrophotometry and multivariate calibration: Stability-indicating analytical tools for the quantification of pimozide in bulk and pharmaceutical dosage form |
| title_short | Univariate spectrophotometry and multivariate calibration: Stability-indicating analytical tools for the quantification of pimozide in bulk and pharmaceutical dosage form |
| title_sort | univariate spectrophotometry and multivariate calibration stability indicating analytical tools for the quantification of pimozide in bulk and pharmaceutical dosage form |
| topic | Pimozide Stability Spectrophotometry Chemometric methods |
| url | http://www.sciencedirect.com/science/article/pii/S1110093115000307 |
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