Compensatory sequence variation between trans-species small RNAs and their target sites
Trans-species small regulatory RNAs (sRNAs) are delivered to host plants from diverse pathogens and parasites and can target host mRNAs. How trans-species sRNAs can be effective on diverse hosts has been unclear. Multiple species of the parasitic plant Cuscuta produce trans-species sRNAs that collec...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2019-12-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/49750 |
| _version_ | 1811180748020908032 |
|---|---|
| author | Nathan R Johnson Claude W dePamphilis Michael J Axtell |
| author_facet | Nathan R Johnson Claude W dePamphilis Michael J Axtell |
| author_sort | Nathan R Johnson |
| collection | DOAJ |
| description | Trans-species small regulatory RNAs (sRNAs) are delivered to host plants from diverse pathogens and parasites and can target host mRNAs. How trans-species sRNAs can be effective on diverse hosts has been unclear. Multiple species of the parasitic plant Cuscuta produce trans-species sRNAs that collectively target many host mRNAs. Confirmed target sites are nearly always in highly conserved, protein-coding regions of host mRNAs. Cuscuta trans-species sRNAs can be grouped into superfamilies that have variation in a three-nucleotide period. These variants compensate for synonymous-site variation in host mRNAs. By targeting host mRNAs at highly conserved protein-coding sites, and simultaneously expressing multiple variants to cover synonymous-site variation, Cuscuta trans-species sRNAs may be able to successfully target multiple homologous mRNAs from diverse hosts. |
| first_indexed | 2024-04-11T09:07:22Z |
| format | Article |
| id | doaj.art-8ebc80e3dadc4b4e91e032ac394b72e8 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:07:22Z |
| publishDate | 2019-12-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-8ebc80e3dadc4b4e91e032ac394b72e82022-12-22T04:32:35ZengeLife Sciences Publications LtdeLife2050-084X2019-12-01810.7554/eLife.49750Compensatory sequence variation between trans-species small RNAs and their target sitesNathan R Johnson0Claude W dePamphilis1Michael J Axtell2https://orcid.org/0000-0001-8951-7361Intercollege PhD Program in Plant Biology, Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, United States; Department of Biology, The Pennsylvania State University, University Park, United StatesIntercollege PhD Program in Plant Biology, Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, United States; Department of Biology, The Pennsylvania State University, University Park, United StatesIntercollege PhD Program in Plant Biology, Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, United States; Department of Biology, The Pennsylvania State University, University Park, United StatesTrans-species small regulatory RNAs (sRNAs) are delivered to host plants from diverse pathogens and parasites and can target host mRNAs. How trans-species sRNAs can be effective on diverse hosts has been unclear. Multiple species of the parasitic plant Cuscuta produce trans-species sRNAs that collectively target many host mRNAs. Confirmed target sites are nearly always in highly conserved, protein-coding regions of host mRNAs. Cuscuta trans-species sRNAs can be grouped into superfamilies that have variation in a three-nucleotide period. These variants compensate for synonymous-site variation in host mRNAs. By targeting host mRNAs at highly conserved protein-coding sites, and simultaneously expressing multiple variants to cover synonymous-site variation, Cuscuta trans-species sRNAs may be able to successfully target multiple homologous mRNAs from diverse hosts.https://elifesciences.org/articles/49750CuscutamicroRNAsiRNA |
| spellingShingle | Nathan R Johnson Claude W dePamphilis Michael J Axtell Compensatory sequence variation between trans-species small RNAs and their target sites eLife Cuscuta microRNA siRNA |
| title | Compensatory sequence variation between trans-species small RNAs and their target sites |
| title_full | Compensatory sequence variation between trans-species small RNAs and their target sites |
| title_fullStr | Compensatory sequence variation between trans-species small RNAs and their target sites |
| title_full_unstemmed | Compensatory sequence variation between trans-species small RNAs and their target sites |
| title_short | Compensatory sequence variation between trans-species small RNAs and their target sites |
| title_sort | compensatory sequence variation between trans species small rnas and their target sites |
| topic | Cuscuta microRNA siRNA |
| url | https://elifesciences.org/articles/49750 |
| work_keys_str_mv | AT nathanrjohnson compensatorysequencevariationbetweentransspeciessmallrnasandtheirtargetsites AT claudewdepamphilis compensatorysequencevariationbetweentransspeciessmallrnasandtheirtargetsites AT michaeljaxtell compensatorysequencevariationbetweentransspeciessmallrnasandtheirtargetsites |