Isolation and Characterization of Microsatellite Loci in the Invasive Herb <i>Solidago altissima</i> (Asteraceae)

Premise of the study: Microsatellite markers were developed in the perennial herb <i>Solidago altissima</i> from populations within its introduced range in Japan to assess its population structure and to facilitate tracking of invasion expansion. Methods and Results: Using 454 pyroseq...

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Bibliographic Details
Main Authors: Yuzu Sakata, Shingo Kaneko, Azusa Hayano, Miho Inoue-Murayama, Takayuki Ohgushi, Yuji Isagi
Format: Article
Language:English
Published: Wiley 2013-04-01
Series:Applications in Plant Sciences
Subjects:
Online Access:http://www.bioone.org/doi/full/10.3732/apps.1200313
Description
Summary:Premise of the study: Microsatellite markers were developed in the perennial herb <i>Solidago altissima</i> from populations within its introduced range in Japan to assess its population structure and to facilitate tracking of invasion expansion. Methods and Results: Using 454 pyrosequencing, 16 microsatellite primer sets were developed for <i>S. altissima</i>. The primer sets were tested on 70 individuals sampled from three populations in Japan. The primers amplified di- and trinucleotide repeats with five to 25 alleles per locus, and the expected heterozygosity ranged from 0.46 to 0.92. Conclusions: These results indicate the utility of primers in <i>S. altissima</i> for future research on a wide range of applications, including tracking of invasion dynamics and investigating population genetics of the species.
ISSN:2168-0450