Characterization of Polyphenols and Volatile Compounds from Understudied Algerian <i>Pallenis spinosa</i> by HS-SPME-GC-MS, NMR and HPLC-MS<sup>n</sup> Approaches
<i>Pallenis spinosa</i> (L.) Cass. is a widespread plant in the Mediterranean region. Traditionally, it is used as a medicinal species to treat several ailments, from inflammation to skin injuries. Although the phytochemical content of this plant has already been investigated, there is c...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-09-01
|
| Series: | Applied Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3417/13/18/10113 |
| _version_ | 1827727380555235328 |
|---|---|
| author | Nabila Adoui Nabila Souilah Hamdi Bendif Stefania Sut Stefano Dall’Acqua Guido Flamini Filippo Maggi Gregorio Peron |
| author_facet | Nabila Adoui Nabila Souilah Hamdi Bendif Stefania Sut Stefano Dall’Acqua Guido Flamini Filippo Maggi Gregorio Peron |
| author_sort | Nabila Adoui |
| collection | DOAJ |
| description | <i>Pallenis spinosa</i> (L.) Cass. is a widespread plant in the Mediterranean region. Traditionally, it is used as a medicinal species to treat several ailments, from inflammation to skin injuries. Although the phytochemical content of this plant has already been investigated, there is currently limited data on Algerian <i>P. spinosa</i>. In this work, we focused on volatile compounds and non-volatile secondary metabolites extracted using HS-SPME and methanol from the aerial parts of <i>P. spinosa</i> collected from Northeast Algeria. Volatile constituents were analyzed by GC-MS, while non-volatile compounds were analyzed by NMR and HPLC-MS<sup>n</sup>. In total, 48 volatile compounds were identified, including sesquiterpene hydrocarbons (65.8%), monoterpene hydrocarbons (16.9%), and oxygenated monoterpenes and sesquiterpenes (8.3% and 6.5%, respectively). β-Chamigrene (16.2%), α-selinene (12.8%), β-pinene (10.6%), and β-caryophyllene (9.2%) were assessed as the main constituents. Concerning non-volatile metabolites, 23 polyphenols were identified (7.26 mg/g DW), and phenolic acids were predominant (5.83 mg/g DW). Tricaffeoylhexaric acid (1.76 mg/g DW), tetracaffeoylhexaric acid (1.41 mg/g DW), 3,5-dicaffeoylquinic acid (1.04 mg/g DW), caffeoyl dihexoside (0.35 mg/g DW), and chlorogenic acid (0.29 mg/g DW) were the most abundant ones. Several known flavonoids, such as tricin and patuletin glycosides, kaempferol, and apigenin, were also identified, and myricetin hexoside was detected in <i>P. spinosa</i> for the first time. Overall, our work is the first to report an exhaustive characterization of volatile and non-volatile secondary metabolites from Algerian <i>P. spinosa</i>. The results represent a step forward in revealing the chemistry of this widespread plant species. Furthermore, they may contribute to rationalizing its traditional medicinal applications and preserve the biodiversity of Algerian flora. |
| first_indexed | 2024-03-10T23:05:37Z |
| format | Article |
| id | doaj.art-8ebed703fbb04f218366963d16bbe083 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T23:05:37Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-8ebed703fbb04f218366963d16bbe0832023-11-19T09:23:14ZengMDPI AGApplied Sciences2076-34172023-09-0113181011310.3390/app131810113Characterization of Polyphenols and Volatile Compounds from Understudied Algerian <i>Pallenis spinosa</i> by HS-SPME-GC-MS, NMR and HPLC-MS<sup>n</sup> ApproachesNabila Adoui0Nabila Souilah1Hamdi Bendif2Stefania Sut3Stefano Dall’Acqua4Guido Flamini5Filippo Maggi6Gregorio Peron7Natural and Life Sciences Department, Faculty of Sciences, University of M’sila, BP 166, Msila 28000, AlgeriaDepartment of Agronomic Sciences, Faculty of Sciences, University of Skikda, BP 26 Road of El Hadaiek, Skikda 21000, AlgeriaNatural and Life Sciences Department, Faculty of Sciences, University of M’sila, BP 166, Msila 28000, AlgeriaDepartment of Pharmaceutical and Pharmacological Sciences, University of Padova, Via Marzolo 5, 35100 Padova, ItalyDepartment of Pharmaceutical and Pharmacological Sciences, University of Padova, Via Marzolo 5, 35100 Padova, ItalyDepartment of Pharmacy, University of Pisa, Via Bonanno 6, 56126 Pisa, ItalySchool of Pharmacy, University of Camerino, Via S. Agostino 1, 62032 Camerino, ItalyDepartment of Molecular and Translational Medicine (DMMT), University of Brescia, Viale Europa 11, 25123 Brescia, Italy<i>Pallenis spinosa</i> (L.) Cass. is a widespread plant in the Mediterranean region. Traditionally, it is used as a medicinal species to treat several ailments, from inflammation to skin injuries. Although the phytochemical content of this plant has already been investigated, there is currently limited data on Algerian <i>P. spinosa</i>. In this work, we focused on volatile compounds and non-volatile secondary metabolites extracted using HS-SPME and methanol from the aerial parts of <i>P. spinosa</i> collected from Northeast Algeria. Volatile constituents were analyzed by GC-MS, while non-volatile compounds were analyzed by NMR and HPLC-MS<sup>n</sup>. In total, 48 volatile compounds were identified, including sesquiterpene hydrocarbons (65.8%), monoterpene hydrocarbons (16.9%), and oxygenated monoterpenes and sesquiterpenes (8.3% and 6.5%, respectively). β-Chamigrene (16.2%), α-selinene (12.8%), β-pinene (10.6%), and β-caryophyllene (9.2%) were assessed as the main constituents. Concerning non-volatile metabolites, 23 polyphenols were identified (7.26 mg/g DW), and phenolic acids were predominant (5.83 mg/g DW). Tricaffeoylhexaric acid (1.76 mg/g DW), tetracaffeoylhexaric acid (1.41 mg/g DW), 3,5-dicaffeoylquinic acid (1.04 mg/g DW), caffeoyl dihexoside (0.35 mg/g DW), and chlorogenic acid (0.29 mg/g DW) were the most abundant ones. Several known flavonoids, such as tricin and patuletin glycosides, kaempferol, and apigenin, were also identified, and myricetin hexoside was detected in <i>P. spinosa</i> for the first time. Overall, our work is the first to report an exhaustive characterization of volatile and non-volatile secondary metabolites from Algerian <i>P. spinosa</i>. The results represent a step forward in revealing the chemistry of this widespread plant species. Furthermore, they may contribute to rationalizing its traditional medicinal applications and preserve the biodiversity of Algerian flora.https://www.mdpi.com/2076-3417/13/18/10113<i>Pallenis spinosa</i>secondary metabolitesterpenespolyphenolsheadspace analysismass spectrometry |
| spellingShingle | Nabila Adoui Nabila Souilah Hamdi Bendif Stefania Sut Stefano Dall’Acqua Guido Flamini Filippo Maggi Gregorio Peron Characterization of Polyphenols and Volatile Compounds from Understudied Algerian <i>Pallenis spinosa</i> by HS-SPME-GC-MS, NMR and HPLC-MS<sup>n</sup> Approaches Applied Sciences <i>Pallenis spinosa</i> secondary metabolites terpenes polyphenols headspace analysis mass spectrometry |
| title | Characterization of Polyphenols and Volatile Compounds from Understudied Algerian <i>Pallenis spinosa</i> by HS-SPME-GC-MS, NMR and HPLC-MS<sup>n</sup> Approaches |
| title_full | Characterization of Polyphenols and Volatile Compounds from Understudied Algerian <i>Pallenis spinosa</i> by HS-SPME-GC-MS, NMR and HPLC-MS<sup>n</sup> Approaches |
| title_fullStr | Characterization of Polyphenols and Volatile Compounds from Understudied Algerian <i>Pallenis spinosa</i> by HS-SPME-GC-MS, NMR and HPLC-MS<sup>n</sup> Approaches |
| title_full_unstemmed | Characterization of Polyphenols and Volatile Compounds from Understudied Algerian <i>Pallenis spinosa</i> by HS-SPME-GC-MS, NMR and HPLC-MS<sup>n</sup> Approaches |
| title_short | Characterization of Polyphenols and Volatile Compounds from Understudied Algerian <i>Pallenis spinosa</i> by HS-SPME-GC-MS, NMR and HPLC-MS<sup>n</sup> Approaches |
| title_sort | characterization of polyphenols and volatile compounds from understudied algerian i pallenis spinosa i by hs spme gc ms nmr and hplc ms sup n sup approaches |
| topic | <i>Pallenis spinosa</i> secondary metabolites terpenes polyphenols headspace analysis mass spectrometry |
| url | https://www.mdpi.com/2076-3417/13/18/10113 |
| work_keys_str_mv | AT nabilaadoui characterizationofpolyphenolsandvolatilecompoundsfromunderstudiedalgerianipallenisspinosaibyhsspmegcmsnmrandhplcmssupnsupapproaches AT nabilasouilah characterizationofpolyphenolsandvolatilecompoundsfromunderstudiedalgerianipallenisspinosaibyhsspmegcmsnmrandhplcmssupnsupapproaches AT hamdibendif characterizationofpolyphenolsandvolatilecompoundsfromunderstudiedalgerianipallenisspinosaibyhsspmegcmsnmrandhplcmssupnsupapproaches AT stefaniasut characterizationofpolyphenolsandvolatilecompoundsfromunderstudiedalgerianipallenisspinosaibyhsspmegcmsnmrandhplcmssupnsupapproaches AT stefanodallacqua characterizationofpolyphenolsandvolatilecompoundsfromunderstudiedalgerianipallenisspinosaibyhsspmegcmsnmrandhplcmssupnsupapproaches AT guidoflamini characterizationofpolyphenolsandvolatilecompoundsfromunderstudiedalgerianipallenisspinosaibyhsspmegcmsnmrandhplcmssupnsupapproaches AT filippomaggi characterizationofpolyphenolsandvolatilecompoundsfromunderstudiedalgerianipallenisspinosaibyhsspmegcmsnmrandhplcmssupnsupapproaches AT gregorioperon characterizationofpolyphenolsandvolatilecompoundsfromunderstudiedalgerianipallenisspinosaibyhsspmegcmsnmrandhplcmssupnsupapproaches |