The role of reactive oxygen species and redoxsensitive protein kinases in the infarction-limiting effect of opioid peptide deltorphin II in cardiac reperfusion in rats
Background. Mortality from acute myocardial infarction with ST-segment elevation in cardiac hospitals ranges from 4.5 to 7 %, and these data has not decreased in recent years. The most common cause of death in patients is cardiogenic shock, the likelihood of which directly depends on infarct size. I...
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| Format: | Article |
| Language: | Russian |
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Scientific Сentre for Family Health and Human Reproduction Problems
2023-05-01
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| Series: | Acta Biomedica Scientifica |
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| Online Access: | https://www.actabiomedica.ru/jour/article/view/4092 |
| _version_ | 1797237145717440512 |
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| author | S. V. Popov A. V. Mukhomedzyanov M. Sirotina B. K. Kurbatov V. N. Azev G. Z. Sufianova M. S. Khlestkina L. N. Maslov |
| author_facet | S. V. Popov A. V. Mukhomedzyanov M. Sirotina B. K. Kurbatov V. N. Azev G. Z. Sufianova M. S. Khlestkina L. N. Maslov |
| author_sort | S. V. Popov |
| collection | DOAJ |
| description | Background. Mortality from acute myocardial infarction with ST-segment elevation in cardiac hospitals ranges from 4.5 to 7 %, and these data has not decreased in recent years. The most common cause of death in patients is cardiogenic shock, the likelihood of which directly depends on infarct size. It is quite clear that there is an urgent need to create drugs to limit the size of infarction and prevent the occurrence of cardiogenic shock.The aim. To evaluate the role of reactive oxygen species and redox-sensitive protein kinases in the infarction-limiting effect of opioid peptide deltorphin II in cardiac reperfusion in rats.Materials and methods. Coronary occlusion (45 min) and reperfusion (120 min) were performed in rats anesthetized with α-chloralose. The selective δ2-opioid receptor agonist deltorphin II, a hydroxyl radical scavenger 2-mercaptoprpionyl glycine (2-MPG), a superoxide radical scavenger tempol, the protein kinase Cδ (PKCδ) inhibitor rottlerin, the PI3-kinase inhibitor wortmannin, the inhibitor of ERK1/2 kinase PD98059 were injected before of reperfusion of the heart.Results. Deltorphin II contributed to a two-fold decrease in infarction size. Injection of 2-MPG, tempol, rottlerin, wortmannin, PD98059 alone had no effect on infarction size in rats. 2-MPG and tempol did not affect the infarction-reducing effect of deltorphin II. Rottlerin, wortmannin, and PD98059 eliminated the cardioprotective effect of deltorphin II.Conclusion. The infarction-reducing effect of deltorphin II does not depend on the production of superoxide radical and hydroxyl radical. Superoxide radical and hydroxyl radical do not play a significant role in reperfusion injury of the heart after coronary occlusion (45 min). PKCδ, PI3-kinase, and ERK1/2 kinase are involved in the infarction-limiting effect of deltorphin II in myocardial reperfusion. |
| first_indexed | 2024-03-08T12:15:05Z |
| format | Article |
| id | doaj.art-8ec589560862453ba66a831e9bb1d43d |
| institution | Directory Open Access Journal |
| issn | 2541-9420 2587-9596 |
| language | Russian |
| last_indexed | 2024-04-24T17:15:06Z |
| publishDate | 2023-05-01 |
| publisher | Scientific Сentre for Family Health and Human Reproduction Problems |
| record_format | Article |
| series | Acta Biomedica Scientifica |
| spelling | doaj.art-8ec589560862453ba66a831e9bb1d43d2024-03-28T07:49:28ZrusScientific Сentre for Family Health and Human Reproduction ProblemsActa Biomedica Scientifica2541-94202587-95962023-05-018225426210.29413/ABS.2023-8.2.252546The role of reactive oxygen species and redoxsensitive protein kinases in the infarction-limiting effect of opioid peptide deltorphin II in cardiac reperfusion in ratsS. V. Popov0A. V. Mukhomedzyanov1M. Sirotina2B. K. Kurbatov3V. N. Azev4G. Z. Sufianova5M. S. Khlestkina6L. N. Maslov7Cardiology Research Institute, Tomsk National Research Medical Center, Russian Academy of SciencesCardiology Research Institute, Tomsk National Research Medical Center, Russian Academy of SciencesCardiology Research Institute, Tomsk National Research Medical Center, Russian Academy of SciencesCardiology Research Institute, Tomsk National Research Medical Center, Russian Academy of SciencesBranch of Shemyakin – Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of SciencesTyumen State Medical UniversityTyumen State Medical UniversityCardiology Research Institute, Tomsk National Research Medical Center, Russian Academy of SciencesBackground. Mortality from acute myocardial infarction with ST-segment elevation in cardiac hospitals ranges from 4.5 to 7 %, and these data has not decreased in recent years. The most common cause of death in patients is cardiogenic shock, the likelihood of which directly depends on infarct size. It is quite clear that there is an urgent need to create drugs to limit the size of infarction and prevent the occurrence of cardiogenic shock.The aim. To evaluate the role of reactive oxygen species and redox-sensitive protein kinases in the infarction-limiting effect of opioid peptide deltorphin II in cardiac reperfusion in rats.Materials and methods. Coronary occlusion (45 min) and reperfusion (120 min) were performed in rats anesthetized with α-chloralose. The selective δ2-opioid receptor agonist deltorphin II, a hydroxyl radical scavenger 2-mercaptoprpionyl glycine (2-MPG), a superoxide radical scavenger tempol, the protein kinase Cδ (PKCδ) inhibitor rottlerin, the PI3-kinase inhibitor wortmannin, the inhibitor of ERK1/2 kinase PD98059 were injected before of reperfusion of the heart.Results. Deltorphin II contributed to a two-fold decrease in infarction size. Injection of 2-MPG, tempol, rottlerin, wortmannin, PD98059 alone had no effect on infarction size in rats. 2-MPG and tempol did not affect the infarction-reducing effect of deltorphin II. Rottlerin, wortmannin, and PD98059 eliminated the cardioprotective effect of deltorphin II.Conclusion. The infarction-reducing effect of deltorphin II does not depend on the production of superoxide radical and hydroxyl radical. Superoxide radical and hydroxyl radical do not play a significant role in reperfusion injury of the heart after coronary occlusion (45 min). PKCδ, PI3-kinase, and ERK1/2 kinase are involved in the infarction-limiting effect of deltorphin II in myocardial reperfusion.https://www.actabiomedica.ru/jour/article/view/4092heartischemiareperfusionopioid receptorsreactive oxygen specieskinases |
| spellingShingle | S. V. Popov A. V. Mukhomedzyanov M. Sirotina B. K. Kurbatov V. N. Azev G. Z. Sufianova M. S. Khlestkina L. N. Maslov The role of reactive oxygen species and redoxsensitive protein kinases in the infarction-limiting effect of opioid peptide deltorphin II in cardiac reperfusion in rats Acta Biomedica Scientifica heart ischemia reperfusion opioid receptors reactive oxygen species kinases |
| title | The role of reactive oxygen species and redoxsensitive protein kinases in the infarction-limiting effect of opioid peptide deltorphin II in cardiac reperfusion in rats |
| title_full | The role of reactive oxygen species and redoxsensitive protein kinases in the infarction-limiting effect of opioid peptide deltorphin II in cardiac reperfusion in rats |
| title_fullStr | The role of reactive oxygen species and redoxsensitive protein kinases in the infarction-limiting effect of opioid peptide deltorphin II in cardiac reperfusion in rats |
| title_full_unstemmed | The role of reactive oxygen species and redoxsensitive protein kinases in the infarction-limiting effect of opioid peptide deltorphin II in cardiac reperfusion in rats |
| title_short | The role of reactive oxygen species and redoxsensitive protein kinases in the infarction-limiting effect of opioid peptide deltorphin II in cardiac reperfusion in rats |
| title_sort | role of reactive oxygen species and redoxsensitive protein kinases in the infarction limiting effect of opioid peptide deltorphin ii in cardiac reperfusion in rats |
| topic | heart ischemia reperfusion opioid receptors reactive oxygen species kinases |
| url | https://www.actabiomedica.ru/jour/article/view/4092 |
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