Establishment and assessment of an amplicon sequencing method targeting the 16S-ITS-23S rRNA operon for analysis of the equine gut microbiome
Abstract Microbial communities are commonly studied by using amplicon sequencing of part of the 16S rRNA gene. Sequencing of the full-length 16S rRNA gene can provide higher taxonomic resolution and accuracy. To obtain even higher taxonomic resolution, with as few false-positives as possible, we ass...
Main Authors: | Yuta Kinoshita, Hidekazu Niwa, Eri Uchida-Fujii, Toshio Nukada |
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Format: | Article |
Language: | English |
Published: |
Nature Portfolio
2021-06-01
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Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-021-91425-7 |
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