Effects of grain boundaries on the biocompatibility of the pure magnesium
The influences of the grain size on the biocompatibility and biodegradation behavior of an extruded and post-annealed pure Mg were investigated. The immersion test, electrochemical analysis, and surface characterization were carried out to study the degradation behavior, and the Bromodeoxyuridine (B...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Journal of Materials Research and Technology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2238785423030570 |
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| author | Mojtaba Fatehi Mollayousef Ahmad Bahmani Mehdi Malekan Mehrab Lotfpour Rouhollah Mehdinavaz Aghdam Fatemeh Asl Zaeem Soraya Bornay Zonoozi Arash Montazeri |
| author_facet | Mojtaba Fatehi Mollayousef Ahmad Bahmani Mehdi Malekan Mehrab Lotfpour Rouhollah Mehdinavaz Aghdam Fatemeh Asl Zaeem Soraya Bornay Zonoozi Arash Montazeri |
| author_sort | Mojtaba Fatehi Mollayousef |
| collection | DOAJ |
| description | The influences of the grain size on the biocompatibility and biodegradation behavior of an extruded and post-annealed pure Mg were investigated. The immersion test, electrochemical analysis, and surface characterization were carried out to study the degradation behavior, and the Bromodeoxyuridine (BrdU) and cell adhesion assays were applied to investigate the cells interaction with the substrate. The results depicted a striking influence of the grain size variations on the degradation behavior, cell viability, and cell adhesion characteristics of the pure magnesium. Here, the grain size was increased from 17.05 ± 2.64 μm for exruded sample to 30.62 ± 3.18 μm for the sample annealed at 400 °C and the degradation rate measured by hydrogen evolution in simulated body fluid (SBF) method increased from 2.42 ± 0.65 to 9.59 ± 1.33 mm/y, respectively. Moreover viability calculated by BrdU assey showed a decrease from 68 % (75 % extract) and 64.5 % (100 % extract) for extruded sample to 57 % (75 % extract) and 58 % (100 % extract) for the sample annealed at 400 °C. Higher annealing temperatures and consequent greater grain size were found to negatively influence the cytocompatibility of the pure Mg. In fact, annealing at the optimum temperature of 300 °C reduced the biodegradation rate by providing a uniform and stable MgO/Ca–P containing surface layer and hence resulted in higher cell adhesion and less cell toxicity. |
| first_indexed | 2024-03-08T09:28:58Z |
| format | Article |
| id | doaj.art-8ed477afd15b427db5051193f74c28b0 |
| institution | Directory Open Access Journal |
| issn | 2238-7854 |
| language | English |
| last_indexed | 2024-03-08T09:28:58Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Materials Research and Technology |
| spelling | doaj.art-8ed477afd15b427db5051193f74c28b02024-01-31T05:43:13ZengElsevierJournal of Materials Research and Technology2238-78542024-01-012811211136Effects of grain boundaries on the biocompatibility of the pure magnesiumMojtaba Fatehi Mollayousef0Ahmad Bahmani1Mehdi Malekan2Mehrab Lotfpour3Rouhollah Mehdinavaz Aghdam4Fatemeh Asl Zaeem5Soraya Bornay Zonoozi6Arash Montazeri7School of Metallurgy and Materials Engineering, College of Engineering, University of Tehran, P.O. Box 11155-4563, Tehran, IranDepartment of Advanced Materials and Renewable Energy, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran; Corresponding author.School of Metallurgy and Materials Engineering, College of Engineering, University of Tehran, P.O. Box 11155-4563, Tehran, Iran; Corresponding author.School of Metallurgy and Materials Engineering, College of Engineering, University of Tehran, P.O. Box 11155-4563, Tehran, IranSchool of Metallurgy and Materials Engineering, College of Engineering, University of Tehran, P.O. Box 11155-4563, Tehran, IranSchool of Biology, University of Tehran, P.O. Box 11155-4563, Tehran, IranMaterials and Energy Research Center (MERC), Alborz, IranNanotechnology Department, Faculty of Engineering, University of Guilan, Rasht, IranThe influences of the grain size on the biocompatibility and biodegradation behavior of an extruded and post-annealed pure Mg were investigated. The immersion test, electrochemical analysis, and surface characterization were carried out to study the degradation behavior, and the Bromodeoxyuridine (BrdU) and cell adhesion assays were applied to investigate the cells interaction with the substrate. The results depicted a striking influence of the grain size variations on the degradation behavior, cell viability, and cell adhesion characteristics of the pure magnesium. Here, the grain size was increased from 17.05 ± 2.64 μm for exruded sample to 30.62 ± 3.18 μm for the sample annealed at 400 °C and the degradation rate measured by hydrogen evolution in simulated body fluid (SBF) method increased from 2.42 ± 0.65 to 9.59 ± 1.33 mm/y, respectively. Moreover viability calculated by BrdU assey showed a decrease from 68 % (75 % extract) and 64.5 % (100 % extract) for extruded sample to 57 % (75 % extract) and 58 % (100 % extract) for the sample annealed at 400 °C. Higher annealing temperatures and consequent greater grain size were found to negatively influence the cytocompatibility of the pure Mg. In fact, annealing at the optimum temperature of 300 °C reduced the biodegradation rate by providing a uniform and stable MgO/Ca–P containing surface layer and hence resulted in higher cell adhesion and less cell toxicity.http://www.sciencedirect.com/science/article/pii/S2238785423030570MagnesiumAnnealingGrain sizeDegradationBiocompatibilityOsteogenesis |
| spellingShingle | Mojtaba Fatehi Mollayousef Ahmad Bahmani Mehdi Malekan Mehrab Lotfpour Rouhollah Mehdinavaz Aghdam Fatemeh Asl Zaeem Soraya Bornay Zonoozi Arash Montazeri Effects of grain boundaries on the biocompatibility of the pure magnesium Journal of Materials Research and Technology Magnesium Annealing Grain size Degradation Biocompatibility Osteogenesis |
| title | Effects of grain boundaries on the biocompatibility of the pure magnesium |
| title_full | Effects of grain boundaries on the biocompatibility of the pure magnesium |
| title_fullStr | Effects of grain boundaries on the biocompatibility of the pure magnesium |
| title_full_unstemmed | Effects of grain boundaries on the biocompatibility of the pure magnesium |
| title_short | Effects of grain boundaries on the biocompatibility of the pure magnesium |
| title_sort | effects of grain boundaries on the biocompatibility of the pure magnesium |
| topic | Magnesium Annealing Grain size Degradation Biocompatibility Osteogenesis |
| url | http://www.sciencedirect.com/science/article/pii/S2238785423030570 |
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