Antihypertensive Effects of IGTGIPGIW Peptide Purified from <i>Hippocampus abdominalis</i>: p-eNOS and p-AKT Stimulation in EA.hy926 Cells and Lowering of Blood Pressure in SHR Model
The aim of this study was to assess the potential hypertensive effects of the IGTGIPGIW peptide purified from <i>Hippocampus abdominalis</i> alcalase hydrolysate (HA) for application in the functional food industry. We investigated the antihypertensive effects of IGTGIPGIW in vitro by as...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/20/6/354 |
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| author | Hyo-Geun Lee Hyun-Soo Kim Hyesuck An Kyunghwa Baek Jeong Min Lee Mi-Jin Yim Seok-Chun Ko Ji-Yul Kim Gun-Woo Oh Jun-Geon Je Dae-Sung Lee You-Jin Jeon |
| author_facet | Hyo-Geun Lee Hyun-Soo Kim Hyesuck An Kyunghwa Baek Jeong Min Lee Mi-Jin Yim Seok-Chun Ko Ji-Yul Kim Gun-Woo Oh Jun-Geon Je Dae-Sung Lee You-Jin Jeon |
| author_sort | Hyo-Geun Lee |
| collection | DOAJ |
| description | The aim of this study was to assess the potential hypertensive effects of the IGTGIPGIW peptide purified from <i>Hippocampus abdominalis</i> alcalase hydrolysate (HA) for application in the functional food industry. We investigated the antihypertensive effects of IGTGIPGIW in vitro by assessing nitric oxide production in EA.hy926 endothelial cells, which is a major factor affecting vasorelaxation. The potential vasorelaxation effect was evaluated using 4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate, a fluorescent stain. IGTGIPGIW significantly increased the expression of endothelial-derived relaxing factors, including endothelial nitric oxide synthase and protein kinase B, in EA.hy926 cells. Furthermore, oral administration of IGTGIPGIW significantly lowered the systolic blood pressure (183.60 ± 1.34 mmHg) and rapidly recovered the diastolic blood pressure (143.50 ± 5.55 mmHg) in the spontaneously hypertensive rat model in vivo. Our results demonstrate the antihypertensive activity of the IGTGIPGIW peptide purified from <i>H. abdominalis</i> and indicate its suitability for application in the functional food industry. |
| first_indexed | 2024-03-09T23:13:49Z |
| format | Article |
| id | doaj.art-8ed538c6061043f08392582c3b76dfdc |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-09T23:13:49Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-8ed538c6061043f08392582c3b76dfdc2023-11-23T17:40:46ZengMDPI AGMarine Drugs1660-33972022-05-0120635410.3390/md20060354Antihypertensive Effects of IGTGIPGIW Peptide Purified from <i>Hippocampus abdominalis</i>: p-eNOS and p-AKT Stimulation in EA.hy926 Cells and Lowering of Blood Pressure in SHR ModelHyo-Geun Lee0Hyun-Soo Kim1Hyesuck An2Kyunghwa Baek3Jeong Min Lee4Mi-Jin Yim5Seok-Chun Ko6Ji-Yul Kim7Gun-Woo Oh8Jun-Geon Je9Dae-Sung Lee10You-Jin Jeon11Department of Marine Life Science, Jeju National University, Jeju 63243, KoreaNational Marine Biodiversity Institute of Korea, 75, Jangsan-ro 101-gil, Janghang-eup, Seocheon 33362, KoreaNational Marine Biodiversity Institute of Korea, 75, Jangsan-ro 101-gil, Janghang-eup, Seocheon 33362, KoreaNational Marine Biodiversity Institute of Korea, 75, Jangsan-ro 101-gil, Janghang-eup, Seocheon 33362, KoreaNational Marine Biodiversity Institute of Korea, 75, Jangsan-ro 101-gil, Janghang-eup, Seocheon 33362, KoreaNational Marine Biodiversity Institute of Korea, 75, Jangsan-ro 101-gil, Janghang-eup, Seocheon 33362, KoreaNational Marine Biodiversity Institute of Korea, 75, Jangsan-ro 101-gil, Janghang-eup, Seocheon 33362, KoreaNational Marine Biodiversity Institute of Korea, 75, Jangsan-ro 101-gil, Janghang-eup, Seocheon 33362, KoreaNational Marine Biodiversity Institute of Korea, 75, Jangsan-ro 101-gil, Janghang-eup, Seocheon 33362, KoreaDepartment of Marine Life Science, Jeju National University, Jeju 63243, KoreaNational Marine Biodiversity Institute of Korea, 75, Jangsan-ro 101-gil, Janghang-eup, Seocheon 33362, KoreaDepartment of Marine Life Science, Jeju National University, Jeju 63243, KoreaThe aim of this study was to assess the potential hypertensive effects of the IGTGIPGIW peptide purified from <i>Hippocampus abdominalis</i> alcalase hydrolysate (HA) for application in the functional food industry. We investigated the antihypertensive effects of IGTGIPGIW in vitro by assessing nitric oxide production in EA.hy926 endothelial cells, which is a major factor affecting vasorelaxation. The potential vasorelaxation effect was evaluated using 4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate, a fluorescent stain. IGTGIPGIW significantly increased the expression of endothelial-derived relaxing factors, including endothelial nitric oxide synthase and protein kinase B, in EA.hy926 cells. Furthermore, oral administration of IGTGIPGIW significantly lowered the systolic blood pressure (183.60 ± 1.34 mmHg) and rapidly recovered the diastolic blood pressure (143.50 ± 5.55 mmHg) in the spontaneously hypertensive rat model in vivo. Our results demonstrate the antihypertensive activity of the IGTGIPGIW peptide purified from <i>H. abdominalis</i> and indicate its suitability for application in the functional food industry.https://www.mdpi.com/1660-3397/20/6/354<i>Hippocampus abdominalis</i>EA.hy926peptideantihypertensive activity |
| spellingShingle | Hyo-Geun Lee Hyun-Soo Kim Hyesuck An Kyunghwa Baek Jeong Min Lee Mi-Jin Yim Seok-Chun Ko Ji-Yul Kim Gun-Woo Oh Jun-Geon Je Dae-Sung Lee You-Jin Jeon Antihypertensive Effects of IGTGIPGIW Peptide Purified from <i>Hippocampus abdominalis</i>: p-eNOS and p-AKT Stimulation in EA.hy926 Cells and Lowering of Blood Pressure in SHR Model Marine Drugs <i>Hippocampus abdominalis</i> EA.hy926 peptide antihypertensive activity |
| title | Antihypertensive Effects of IGTGIPGIW Peptide Purified from <i>Hippocampus abdominalis</i>: p-eNOS and p-AKT Stimulation in EA.hy926 Cells and Lowering of Blood Pressure in SHR Model |
| title_full | Antihypertensive Effects of IGTGIPGIW Peptide Purified from <i>Hippocampus abdominalis</i>: p-eNOS and p-AKT Stimulation in EA.hy926 Cells and Lowering of Blood Pressure in SHR Model |
| title_fullStr | Antihypertensive Effects of IGTGIPGIW Peptide Purified from <i>Hippocampus abdominalis</i>: p-eNOS and p-AKT Stimulation in EA.hy926 Cells and Lowering of Blood Pressure in SHR Model |
| title_full_unstemmed | Antihypertensive Effects of IGTGIPGIW Peptide Purified from <i>Hippocampus abdominalis</i>: p-eNOS and p-AKT Stimulation in EA.hy926 Cells and Lowering of Blood Pressure in SHR Model |
| title_short | Antihypertensive Effects of IGTGIPGIW Peptide Purified from <i>Hippocampus abdominalis</i>: p-eNOS and p-AKT Stimulation in EA.hy926 Cells and Lowering of Blood Pressure in SHR Model |
| title_sort | antihypertensive effects of igtgipgiw peptide purified from i hippocampus abdominalis i p enos and p akt stimulation in ea hy926 cells and lowering of blood pressure in shr model |
| topic | <i>Hippocampus abdominalis</i> EA.hy926 peptide antihypertensive activity |
| url | https://www.mdpi.com/1660-3397/20/6/354 |
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