Sensitivity and Regulation of Diel Photosynthesis in Red-Fleshed Pitaya (<i>Hylocereus polyrhizus</i>) Micropropagules under Mannitol-Induced Water Stress/Rehydration Cycle In Vitro
Climate change-induced prolonged water stress (WS) affects crassulacean acid metabolism photosynthesis in pitaya (<i>Hylocereus</i>), limiting crop productivity through insufficient photosynthate. To document how WS/rehydration affects diel photosynthesis, red-fleshed pitaya (<i>H....
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-02-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/10/3/235 |
| _version_ | 1797240828595273728 |
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| author | Yu-Chi Lee Jer-Chia Chang |
| author_facet | Yu-Chi Lee Jer-Chia Chang |
| author_sort | Yu-Chi Lee |
| collection | DOAJ |
| description | Climate change-induced prolonged water stress (WS) affects crassulacean acid metabolism photosynthesis in pitaya (<i>Hylocereus</i>), limiting crop productivity through insufficient photosynthate. To document how WS/rehydration affects diel photosynthesis, red-fleshed pitaya (<i>H. polyrhizus</i>) micropropagules were studied for 5 weeks in a mannitol-induced water potential gradient replaced with moderate (MWS; −1.0 MPa in week 2; −0.5 MPa for the rest) or intensified (IWS; −1.0 and −1.5 MPa in weeks 2 and 3; −0.5 MPa for the rest) WS in vitro. Net photosynthetic rate (Pn) and integrated net CO<sub>2</sub> uptake (INCU) were measured using an Arduino-based photosynthesis system. Micropropagules under MWS had similar Pn in weeks 5 and 1, whereas the control (−0.5 MPa) increased. Pn recovery did not occur after IWS. The average relative INCU was similar in the control and MWS, but lower in IWS. The Pn difference increased with WS, becoming more evident at dawn (Phase II), evening (Phase IV), and predawn the next day (Phase I), and occurred earlier in Phases IV and I under IWS. MWS did not reduce photosynthesis, demonstrating that the photosynthetic regulation could respond to short-term WS in pitaya and indicating the potential of watering for Pn recovery at evening and predawn under IWS. |
| first_indexed | 2024-04-24T18:13:38Z |
| format | Article |
| id | doaj.art-8edadec00ecc48b08020f4e9811cf504 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-04-24T18:13:38Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-8edadec00ecc48b08020f4e9811cf5042024-03-27T13:44:18ZengMDPI AGHorticulturae2311-75242024-02-0110323510.3390/horticulturae10030235Sensitivity and Regulation of Diel Photosynthesis in Red-Fleshed Pitaya (<i>Hylocereus polyrhizus</i>) Micropropagules under Mannitol-Induced Water Stress/Rehydration Cycle In VitroYu-Chi Lee0Jer-Chia Chang1Department of Horticulture, National Chung-Hsing University, No. 145, Xingda Road, South District, Taichung City 402, TaiwanDepartment of Horticulture, National Chung-Hsing University, No. 145, Xingda Road, South District, Taichung City 402, TaiwanClimate change-induced prolonged water stress (WS) affects crassulacean acid metabolism photosynthesis in pitaya (<i>Hylocereus</i>), limiting crop productivity through insufficient photosynthate. To document how WS/rehydration affects diel photosynthesis, red-fleshed pitaya (<i>H. polyrhizus</i>) micropropagules were studied for 5 weeks in a mannitol-induced water potential gradient replaced with moderate (MWS; −1.0 MPa in week 2; −0.5 MPa for the rest) or intensified (IWS; −1.0 and −1.5 MPa in weeks 2 and 3; −0.5 MPa for the rest) WS in vitro. Net photosynthetic rate (Pn) and integrated net CO<sub>2</sub> uptake (INCU) were measured using an Arduino-based photosynthesis system. Micropropagules under MWS had similar Pn in weeks 5 and 1, whereas the control (−0.5 MPa) increased. Pn recovery did not occur after IWS. The average relative INCU was similar in the control and MWS, but lower in IWS. The Pn difference increased with WS, becoming more evident at dawn (Phase II), evening (Phase IV), and predawn the next day (Phase I), and occurred earlier in Phases IV and I under IWS. MWS did not reduce photosynthesis, demonstrating that the photosynthetic regulation could respond to short-term WS in pitaya and indicating the potential of watering for Pn recovery at evening and predawn under IWS.https://www.mdpi.com/2311-7524/10/3/235pitahayacrassulacean acid metabolismdiel CO<sub>2</sub> uptakephotosynthetic patternphasewater stress |
| spellingShingle | Yu-Chi Lee Jer-Chia Chang Sensitivity and Regulation of Diel Photosynthesis in Red-Fleshed Pitaya (<i>Hylocereus polyrhizus</i>) Micropropagules under Mannitol-Induced Water Stress/Rehydration Cycle In Vitro Horticulturae pitahaya crassulacean acid metabolism diel CO<sub>2</sub> uptake photosynthetic pattern phase water stress |
| title | Sensitivity and Regulation of Diel Photosynthesis in Red-Fleshed Pitaya (<i>Hylocereus polyrhizus</i>) Micropropagules under Mannitol-Induced Water Stress/Rehydration Cycle In Vitro |
| title_full | Sensitivity and Regulation of Diel Photosynthesis in Red-Fleshed Pitaya (<i>Hylocereus polyrhizus</i>) Micropropagules under Mannitol-Induced Water Stress/Rehydration Cycle In Vitro |
| title_fullStr | Sensitivity and Regulation of Diel Photosynthesis in Red-Fleshed Pitaya (<i>Hylocereus polyrhizus</i>) Micropropagules under Mannitol-Induced Water Stress/Rehydration Cycle In Vitro |
| title_full_unstemmed | Sensitivity and Regulation of Diel Photosynthesis in Red-Fleshed Pitaya (<i>Hylocereus polyrhizus</i>) Micropropagules under Mannitol-Induced Water Stress/Rehydration Cycle In Vitro |
| title_short | Sensitivity and Regulation of Diel Photosynthesis in Red-Fleshed Pitaya (<i>Hylocereus polyrhizus</i>) Micropropagules under Mannitol-Induced Water Stress/Rehydration Cycle In Vitro |
| title_sort | sensitivity and regulation of diel photosynthesis in red fleshed pitaya i hylocereus polyrhizus i micropropagules under mannitol induced water stress rehydration cycle in vitro |
| topic | pitahaya crassulacean acid metabolism diel CO<sub>2</sub> uptake photosynthetic pattern phase water stress |
| url | https://www.mdpi.com/2311-7524/10/3/235 |
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