Aptamer AS1411-functionalized gold nanoparticle-melittin complex for targeting MCF-7 breast cancer cell line
Objective(s): Several studies reported the apoptotic and lytic activity of melittin (Mel) in different tumor cells. In this study, a novel nano-complex was developed composed of AS1411aptamers, melittin and gold nanoparticle for the treatment of breast cancer cells. Materials and Methods: Gold nanop...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2022-04-01
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| Series: | Nanomedicine Journal |
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| Online Access: | https://nmj.mums.ac.ir/article_19819_ec7a1222162cc56e305e5e1040b6ff9a.pdf |
| _version_ | 1819061032618819584 |
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| author | Payam Bayat Khalil Abnous Soudabeh Balarastaghi Seyed Mohamaad Taghdisi Majid Saeedi Reavan Yazdian-Robati Mahmoud Mahmoudi |
| author_facet | Payam Bayat Khalil Abnous Soudabeh Balarastaghi Seyed Mohamaad Taghdisi Majid Saeedi Reavan Yazdian-Robati Mahmoud Mahmoudi |
| author_sort | Payam Bayat |
| collection | DOAJ |
| description | Objective(s): Several studies reported the apoptotic and lytic activity of melittin (Mel) in different tumor cells. In this study, a novel nano-complex was developed composed of AS1411aptamers, melittin and gold nanoparticle for the treatment of breast cancer cells. Materials and Methods: Gold nanoparticles (GNP) were synthesized using reduction of tetrachloroauric acid (HAuCl4). Melittin modified with cysteine and AS1411aptamer conjugated to the gold nanoparticle. Gel retardation assay was used to prove the formation of GNP-Mel-AS1411 complex. Physicochemical properties of complex were investigated by Dynamic Light Scattering (DLS). The cytotoxicity of Mel and GNP-Mel-AS1411 complex were evaluated in both MCF‐7 (target) and L929 (non-target) cells by the MTT assay. Results: The average size of GNP and GNP-Mel-AS1411 complex were 20 ± 2.5 and 270.5± 3.2 respectively. The results of MTT assay revealed that this nanocomplex was more cytotoxic in MCF‐7 (cell viability = 19% ± 2%) and less cytotoxic in L929 cells (cell viability = 73% ± 1.6%).Conclusion: The results of this study indicated that the gold nanoparticle-melittin-AS1411 complex had a potential value in cancer cell targeted delivery of melittin. |
| first_indexed | 2024-12-21T14:36:26Z |
| format | Article |
| id | doaj.art-8edc3b52f6df4b86807d4d3deae87a6b |
| institution | Directory Open Access Journal |
| issn | 2322-3049 2322-5904 |
| language | English |
| last_indexed | 2024-12-21T14:36:26Z |
| publishDate | 2022-04-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Nanomedicine Journal |
| spelling | doaj.art-8edc3b52f6df4b86807d4d3deae87a6b2022-12-21T19:00:19ZengMashhad University of Medical SciencesNanomedicine Journal2322-30492322-59042022-04-019216416910.22038/nmj.2022.62476.164919819Aptamer AS1411-functionalized gold nanoparticle-melittin complex for targeting MCF-7 breast cancer cell linePayam Bayat0Khalil Abnous1Soudabeh Balarastaghi2Seyed Mohamaad Taghdisi3Majid Saeedi4Reavan Yazdian-Robati5Mahmoud Mahmoudi6Immunology Research Center, BuAli Research Institute, Department of Immunology and Allergy, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Pharmaceutics, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, IranMolecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, IranImmunology Research Center, BuAli Research Institute, Department of Immunology and Allergy, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranObjective(s): Several studies reported the apoptotic and lytic activity of melittin (Mel) in different tumor cells. In this study, a novel nano-complex was developed composed of AS1411aptamers, melittin and gold nanoparticle for the treatment of breast cancer cells. Materials and Methods: Gold nanoparticles (GNP) were synthesized using reduction of tetrachloroauric acid (HAuCl4). Melittin modified with cysteine and AS1411aptamer conjugated to the gold nanoparticle. Gel retardation assay was used to prove the formation of GNP-Mel-AS1411 complex. Physicochemical properties of complex were investigated by Dynamic Light Scattering (DLS). The cytotoxicity of Mel and GNP-Mel-AS1411 complex were evaluated in both MCF‐7 (target) and L929 (non-target) cells by the MTT assay. Results: The average size of GNP and GNP-Mel-AS1411 complex were 20 ± 2.5 and 270.5± 3.2 respectively. The results of MTT assay revealed that this nanocomplex was more cytotoxic in MCF‐7 (cell viability = 19% ± 2%) and less cytotoxic in L929 cells (cell viability = 73% ± 1.6%).Conclusion: The results of this study indicated that the gold nanoparticle-melittin-AS1411 complex had a potential value in cancer cell targeted delivery of melittin.https://nmj.mums.ac.ir/article_19819_ec7a1222162cc56e305e5e1040b6ff9a.pdfas1411aptamergold nanoparticlemelittinnucleolintargeted therapy |
| spellingShingle | Payam Bayat Khalil Abnous Soudabeh Balarastaghi Seyed Mohamaad Taghdisi Majid Saeedi Reavan Yazdian-Robati Mahmoud Mahmoudi Aptamer AS1411-functionalized gold nanoparticle-melittin complex for targeting MCF-7 breast cancer cell line Nanomedicine Journal as1411aptamer gold nanoparticle melittin nucleolin targeted therapy |
| title | Aptamer AS1411-functionalized gold nanoparticle-melittin complex for targeting MCF-7 breast cancer cell line |
| title_full | Aptamer AS1411-functionalized gold nanoparticle-melittin complex for targeting MCF-7 breast cancer cell line |
| title_fullStr | Aptamer AS1411-functionalized gold nanoparticle-melittin complex for targeting MCF-7 breast cancer cell line |
| title_full_unstemmed | Aptamer AS1411-functionalized gold nanoparticle-melittin complex for targeting MCF-7 breast cancer cell line |
| title_short | Aptamer AS1411-functionalized gold nanoparticle-melittin complex for targeting MCF-7 breast cancer cell line |
| title_sort | aptamer as1411 functionalized gold nanoparticle melittin complex for targeting mcf 7 breast cancer cell line |
| topic | as1411aptamer gold nanoparticle melittin nucleolin targeted therapy |
| url | https://nmj.mums.ac.ir/article_19819_ec7a1222162cc56e305e5e1040b6ff9a.pdf |
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