Molecular mechanism of salinity and waterlogging tolerance in mangrove Kandelia obovata
Mangrove forests are colloquially referred to as “Earth’s kidneys” and serve many important ecological and commercial functions. Salinity and waterlogging stress are the most important abiotic stressors restricting the growth and development of mangroves. Kandelia obovata (K. obovata) is the greates...
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| Format: | Article |
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1354249/full |
| _version_ | 1797322504223588352 |
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| author | Huizi Liu Xia An Xing Liu Sheng Yang Yu Liu Xin Wei Xiaowen Li Qiuxia Chen Jinwang Wang |
| author_facet | Huizi Liu Xia An Xing Liu Sheng Yang Yu Liu Xin Wei Xiaowen Li Qiuxia Chen Jinwang Wang |
| author_sort | Huizi Liu |
| collection | DOAJ |
| description | Mangrove forests are colloquially referred to as “Earth’s kidneys” and serve many important ecological and commercial functions. Salinity and waterlogging stress are the most important abiotic stressors restricting the growth and development of mangroves. Kandelia obovata (K. obovata) is the greatest latitudinally-distributed salt mangrove species in China.Here, morphology and transcriptomics were used to study the response of K. obovata to salt and waterlogging stress. In addition, weighted gene co-expression network analysis of the combined gene expression and phenotypic datasets was used to identify core salinity- and waterlogging-responsive modules. In this study, we observed that both high salinity and waterlogging significantly inhibited growth and development in K. obovata. Notably, growth was negatively correlated with salt concentration and positively correlated with waterlogging duration, and high salinity was significantly more inhibitive than waterlogging. A total of 7, 591 salt-responsive and 228 waterlogging-responsive differentially expressed genes were identified by RNA sequencing. Long-term salt stress was highly correlated with the measured physiological parameters while long-term waterlogging was poorly correlated with these traits. At the same time, 45 salinity-responsive and 16 waterlogging-responsive core genes were identified. All 61 core genes were mainly involved in metabolic and biosynthesis of secondary metabolites pathways. This study provides valuable insight into the molecular mechanisms of salinity and waterlogging tolerance in K. obovata, as well as a useful genetic resource for the improvement of mangrove stress tolerance using molecular breeding techniques. |
| first_indexed | 2024-03-08T05:15:14Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-08T05:15:14Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-8edd15f7981e49808df52ac39414d98e2024-02-07T04:37:50ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-02-011510.3389/fpls.2024.13542491354249Molecular mechanism of salinity and waterlogging tolerance in mangrove Kandelia obovataHuizi Liu0Xia An1Xing Liu2Sheng Yang3Yu Liu4Xin Wei5Xiaowen Li6Qiuxia Chen7Jinwang Wang8Zhejiang Institute of Subtropical Crops, Zhejiang Academy of Agricultural Sciences, Wenzhou, ChinaZhejiang Xiaoshan Institute of Cotton and Bast Fiber Crops, Zhejiang Institute of Landscape Plants and Flowers, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaZhejiang Institute of Subtropical Crops, Zhejiang Academy of Agricultural Sciences, Wenzhou, ChinaZhejiang Institute of Subtropical Crops, Zhejiang Academy of Agricultural Sciences, Wenzhou, ChinaZhejiang Institute of Subtropical Crops, Zhejiang Academy of Agricultural Sciences, Wenzhou, ChinaZhejiang Institute of Subtropical Crops, Zhejiang Academy of Agricultural Sciences, Wenzhou, ChinaZhejiang Institute of Subtropical Crops, Zhejiang Academy of Agricultural Sciences, Wenzhou, ChinaZhejiang Institute of Subtropical Crops, Zhejiang Academy of Agricultural Sciences, Wenzhou, ChinaZhejiang Institute of Subtropical Crops, Zhejiang Academy of Agricultural Sciences, Wenzhou, ChinaMangrove forests are colloquially referred to as “Earth’s kidneys” and serve many important ecological and commercial functions. Salinity and waterlogging stress are the most important abiotic stressors restricting the growth and development of mangroves. Kandelia obovata (K. obovata) is the greatest latitudinally-distributed salt mangrove species in China.Here, morphology and transcriptomics were used to study the response of K. obovata to salt and waterlogging stress. In addition, weighted gene co-expression network analysis of the combined gene expression and phenotypic datasets was used to identify core salinity- and waterlogging-responsive modules. In this study, we observed that both high salinity and waterlogging significantly inhibited growth and development in K. obovata. Notably, growth was negatively correlated with salt concentration and positively correlated with waterlogging duration, and high salinity was significantly more inhibitive than waterlogging. A total of 7, 591 salt-responsive and 228 waterlogging-responsive differentially expressed genes were identified by RNA sequencing. Long-term salt stress was highly correlated with the measured physiological parameters while long-term waterlogging was poorly correlated with these traits. At the same time, 45 salinity-responsive and 16 waterlogging-responsive core genes were identified. All 61 core genes were mainly involved in metabolic and biosynthesis of secondary metabolites pathways. This study provides valuable insight into the molecular mechanisms of salinity and waterlogging tolerance in K. obovata, as well as a useful genetic resource for the improvement of mangrove stress tolerance using molecular breeding techniques.https://www.frontiersin.org/articles/10.3389/fpls.2024.1354249/fullKandelia obovatasalt stresswaterlogging stresstranscriptomic analysisweighted gene co-expression network |
| spellingShingle | Huizi Liu Xia An Xing Liu Sheng Yang Yu Liu Xin Wei Xiaowen Li Qiuxia Chen Jinwang Wang Molecular mechanism of salinity and waterlogging tolerance in mangrove Kandelia obovata Frontiers in Plant Science Kandelia obovata salt stress waterlogging stress transcriptomic analysis weighted gene co-expression network |
| title | Molecular mechanism of salinity and waterlogging tolerance in mangrove Kandelia obovata |
| title_full | Molecular mechanism of salinity and waterlogging tolerance in mangrove Kandelia obovata |
| title_fullStr | Molecular mechanism of salinity and waterlogging tolerance in mangrove Kandelia obovata |
| title_full_unstemmed | Molecular mechanism of salinity and waterlogging tolerance in mangrove Kandelia obovata |
| title_short | Molecular mechanism of salinity and waterlogging tolerance in mangrove Kandelia obovata |
| title_sort | molecular mechanism of salinity and waterlogging tolerance in mangrove kandelia obovata |
| topic | Kandelia obovata salt stress waterlogging stress transcriptomic analysis weighted gene co-expression network |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1354249/full |
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