Sequence analysis of mitochondrial fragment COⅡ gene of H. labeo and H. maculates from Oujiang River(瓯江流域唇和花线粒体COⅡ基因部分序列分析)
采用PCR技术获得了唇(Hemibarbus labeo )和花(H. maculatus)线粒体DNA细胞色素氧化酶(COⅡ)基因部分序列,将所得的COⅡ基因序列与4种取自GenBank的属鱼类同一基因序列进行了分析,以探讨这一序列在种质鉴定、种群遗传结构和分子系统发生研究中的应用价值.测序结果表明,在实际分析的600 bp序列中,序列A+T含量(56.9%~57.8%)高于G+C含量,物种间共有变异位点116个,其中简约信息位点46个;唇和花2个地理种群间变异位点均为11个,唇种群间碱基替换均为转换,花的转换/颠换为8/3.以COⅡ基因片段序列为标记,用似刺鳊(Paracanthobram...
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Zhejiang University Press
2008-09-01
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Series: | Zhejiang Daxue xuebao. Lixue ban |
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Online Access: | https://doi.org/10.3785/j.issn.1008-9497.2008.05.023 |
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author | LÜYao-ping(吕耀平) HUZe-hui(胡则辉) YELi-ping(叶丽平) YAOZi-liang(姚子亮) |
author_facet | LÜYao-ping(吕耀平) HUZe-hui(胡则辉) YELi-ping(叶丽平) YAOZi-liang(姚子亮) |
author_sort | LÜYao-ping(吕耀平) |
collection | DOAJ |
description | 采用PCR技术获得了唇(Hemibarbus labeo )和花(H. maculatus)线粒体DNA细胞色素氧化酶(COⅡ)基因部分序列,将所得的COⅡ基因序列与4种取自GenBank的属鱼类同一基因序列进行了分析,以探讨这一序列在种质鉴定、种群遗传结构和分子系统发生研究中的应用价值.测序结果表明,在实际分析的600 bp序列中,序列A+T含量(56.9%~57.8%)高于G+C含量,物种间共有变异位点116个,其中简约信息位点46个;唇和花2个地理种群间变异位点均为11个,唇种群间碱基替换均为转换,花的转换/颠换为8/3.以COⅡ基因片段序列为标记,用似刺鳊(Paracanthobrama guichenoti Bleeker)作外群,构建了属鱼类的系统发生树,其拓扑结构显示2个地理种群浙江花单倍体Ⅰ(H1)与江苏花首先聚为一支,然后与浙江花单倍体Ⅱ (H2)形成一个单系群.韩国唇和日本为姐妹群,然后与中国花(浙江和江苏)相聚为一支.长吻和朝鲜单独聚为一支,表明2者的亲缘关系较近.结果同时表明,韩国唇与江苏花、日本和浙江花(H1)的遗传距离均为0.008 4,结合形态特征鉴定,推测韩国唇和日本是中国花(浙江和江苏)的同物异名. |
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spelling | doaj.art-8edd912d70514d54ae28033ea13e935c2024-03-29T01:58:26ZzhoZhejiang University PressZhejiang Daxue xuebao. Lixue ban1008-94972008-09-0135558058510.3785/j.issn.1008-9497.2008.05.023Sequence analysis of mitochondrial fragment COⅡ gene of H. labeo and H. maculates from Oujiang River(瓯江流域唇和花线粒体COⅡ基因部分序列分析)LÜYao-ping(吕耀平)0HUZe-hui(胡则辉)1YELi-ping(叶丽平)2YAOZi-liang(姚子亮)3 1.College of Chemistry and Life Sciences, Lishui University, Lishui 323000, Zhejiang Province, China( 1.丽水学院化学与生命科学学院,浙江 丽水 323000) 3.Marine Fisheries Research Institute of Zhejiang Province, Zhoushan 316100, Zhejiang Province, China( 3.浙江省海洋水产研究所,浙江 舟山 316100) 1.College of Chemistry and Life Sciences, Lishui University, Lishui 323000, Zhejiang Province, China( 1.丽水学院化学与生命科学学院,浙江 丽水 323000) 4.Lishui Fishery Technology Promotion Station of Zhejiang Province, Lishui 323000, Zhejiang province, China( 4.浙江省丽水市水产技术推广站,浙江 丽水 323000)采用PCR技术获得了唇(Hemibarbus labeo )和花(H. maculatus)线粒体DNA细胞色素氧化酶(COⅡ)基因部分序列,将所得的COⅡ基因序列与4种取自GenBank的属鱼类同一基因序列进行了分析,以探讨这一序列在种质鉴定、种群遗传结构和分子系统发生研究中的应用价值.测序结果表明,在实际分析的600 bp序列中,序列A+T含量(56.9%~57.8%)高于G+C含量,物种间共有变异位点116个,其中简约信息位点46个;唇和花2个地理种群间变异位点均为11个,唇种群间碱基替换均为转换,花的转换/颠换为8/3.以COⅡ基因片段序列为标记,用似刺鳊(Paracanthobrama guichenoti Bleeker)作外群,构建了属鱼类的系统发生树,其拓扑结构显示2个地理种群浙江花单倍体Ⅰ(H1)与江苏花首先聚为一支,然后与浙江花单倍体Ⅱ (H2)形成一个单系群.韩国唇和日本为姐妹群,然后与中国花(浙江和江苏)相聚为一支.长吻和朝鲜单独聚为一支,表明2者的亲缘关系较近.结果同时表明,韩国唇与江苏花、日本和浙江花(H1)的遗传距离均为0.008 4,结合形态特征鉴定,推测韩国唇和日本是中国花(浙江和江苏)的同物异名.https://doi.org/10.3785/j.issn.1008-9497.2008.05.023 属鱼类coⅱ基因系统发育 |
spellingShingle | LÜYao-ping(吕耀平) HUZe-hui(胡则辉) YELi-ping(叶丽平) YAOZi-liang(姚子亮) Sequence analysis of mitochondrial fragment COⅡ gene of H. labeo and H. maculates from Oujiang River(瓯江流域唇和花线粒体COⅡ基因部分序列分析) Zhejiang Daxue xuebao. Lixue ban 属鱼类 coⅱ基因 系统发育 |
title | Sequence analysis of mitochondrial fragment COⅡ gene of H. labeo and H. maculates from Oujiang River(瓯江流域唇和花线粒体COⅡ基因部分序列分析) |
title_full | Sequence analysis of mitochondrial fragment COⅡ gene of H. labeo and H. maculates from Oujiang River(瓯江流域唇和花线粒体COⅡ基因部分序列分析) |
title_fullStr | Sequence analysis of mitochondrial fragment COⅡ gene of H. labeo and H. maculates from Oujiang River(瓯江流域唇和花线粒体COⅡ基因部分序列分析) |
title_full_unstemmed | Sequence analysis of mitochondrial fragment COⅡ gene of H. labeo and H. maculates from Oujiang River(瓯江流域唇和花线粒体COⅡ基因部分序列分析) |
title_short | Sequence analysis of mitochondrial fragment COⅡ gene of H. labeo and H. maculates from Oujiang River(瓯江流域唇和花线粒体COⅡ基因部分序列分析) |
title_sort | sequence analysis of mitochondrial fragment coii gene of h labeo and h maculates from oujiang river 瓯江流域唇和花线粒体coii基因部分序列分析 |
topic | 属鱼类 coⅱ基因 系统发育 |
url | https://doi.org/10.3785/j.issn.1008-9497.2008.05.023 |
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