Detection of p53, PGP9.5, SOX2, GAGE7, GBU4-5 and MAGE A1 protein levels in lung cancer tissues and peripheral blood and their clinical value
Background and purpose: The pathogenesis of lung cancer is diverse, however once confirmed, the best time for treatment is often missed. In this paper, the correlation between tumor suppressor gene (p53), protein gene product (PGP9.5), transcription factor (SOX2), tumor-associated gene protein (GAGE...
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| Format: | Article |
| Language: | English |
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Editorial Office of China Oncology
2023-01-01
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| Series: | Zhongguo aizheng zazhi |
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| Online Access: | http://www.china-oncology.com/fileup/1007-3639/PDF/1676258276072-2126946475.pdf |
| _version_ | 1797894406914703360 |
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| author | ZOU Chunyuan, XU Xiaofeng, LU Renquan, GUO Lin |
| author_facet | ZOU Chunyuan, XU Xiaofeng, LU Renquan, GUO Lin |
| author_sort | ZOU Chunyuan, XU Xiaofeng, LU Renquan, GUO Lin |
| collection | DOAJ |
| description | Background and purpose: The pathogenesis of lung cancer is diverse, however once confirmed, the best time for treatment is often missed. In this paper, the correlation between tumor suppressor gene (p53), protein gene product (PGP9.5), transcription factor (SOX2), tumor-associated gene protein (GAGE7), helicase (GBU4-5) and melanoma antigen (MAGE A1) protein levels in lung cancer tissues and peripheral blood of patients was discussed, and its application value in the diagnosis and treatment of lung cancer was analyzed. Methods: A total of 100 lung cancer patients in Fudan University Shanghai Cancer Center from May 2018 to May 2019 were enrolled, including 25 cases of clinical TNM stage Ⅰ, 45 cases of stage Ⅱ, 30 cases of stage Ⅲa, 80 cases of non-small cell lung cancer and 20 cases of small cell lung cancer. Following surgical removal of intact tumor tissues and cancer-adjacent tissues, immunohistochemical staining was used to detect positive rates of above-mentioned 6 proteins, real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR) was used to detect their gene expression levels, and enzyme-linked immunosorbent assay (ELISA) was performed to detect antibody positive rates in peripheral serum. Results: The positive rates and gene expression levels of p53, PGP9.5, SOX2, GAGE7, GBU4-5 and MAGE A1 in tumor tissues were significantly higher compared with adjacent tissues (P<0.05). Correlation analysis between p53, PGP9.5, SOX2, GAGE7, GBU4-5 and MAGE A1 proteins in tumor tissues and serum showed that the positive rates and gene expression levels of P53, PGP9.5, SOX2, GAGE7, GBU4-5 and MAGE A1 were correlated with TNM stage and differentiation level (P<0.05). There was no significant difference in gender, age, tumor diameter and pathological type (P>0.05). The protein expression levels of p53, PGP9.5, SOX2, GAGE7, GBU4-5 and MAGE A1 in tumor tissues were consistent with their expression levels in peripheral blood (P>0.05). Conclusion: Positive expressions of p53, PGP9.5, SOX2, GAGE7, GBU4-5 and MAGE A1 protein in lung cancer tissues and peripheral serum were closely related to TNM staging and differentiation. |
| first_indexed | 2024-04-10T07:09:31Z |
| format | Article |
| id | doaj.art-8ee077e03fbe44bd91ad8e0862cd5395 |
| institution | Directory Open Access Journal |
| issn | 1007-3639 |
| language | English |
| last_indexed | 2024-04-10T07:09:31Z |
| publishDate | 2023-01-01 |
| publisher | Editorial Office of China Oncology |
| record_format | Article |
| series | Zhongguo aizheng zazhi |
| spelling | doaj.art-8ee077e03fbe44bd91ad8e0862cd53952023-02-27T00:20:41ZengEditorial Office of China OncologyZhongguo aizheng zazhi1007-36392023-01-01331364410.19401/j.cnki.1007-3639.2023.01.004Detection of p53, PGP9.5, SOX2, GAGE7, GBU4-5 and MAGE A1 protein levels in lung cancer tissues and peripheral blood and their clinical valueZOU Chunyuan, XU Xiaofeng, LU Renquan, GUO Lin0Department of Laboratory Diagnosis, Fudan University Shanghai Cancer Center, Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, ChinaBackground and purpose: The pathogenesis of lung cancer is diverse, however once confirmed, the best time for treatment is often missed. In this paper, the correlation between tumor suppressor gene (p53), protein gene product (PGP9.5), transcription factor (SOX2), tumor-associated gene protein (GAGE7), helicase (GBU4-5) and melanoma antigen (MAGE A1) protein levels in lung cancer tissues and peripheral blood of patients was discussed, and its application value in the diagnosis and treatment of lung cancer was analyzed. Methods: A total of 100 lung cancer patients in Fudan University Shanghai Cancer Center from May 2018 to May 2019 were enrolled, including 25 cases of clinical TNM stage Ⅰ, 45 cases of stage Ⅱ, 30 cases of stage Ⅲa, 80 cases of non-small cell lung cancer and 20 cases of small cell lung cancer. Following surgical removal of intact tumor tissues and cancer-adjacent tissues, immunohistochemical staining was used to detect positive rates of above-mentioned 6 proteins, real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR) was used to detect their gene expression levels, and enzyme-linked immunosorbent assay (ELISA) was performed to detect antibody positive rates in peripheral serum. Results: The positive rates and gene expression levels of p53, PGP9.5, SOX2, GAGE7, GBU4-5 and MAGE A1 in tumor tissues were significantly higher compared with adjacent tissues (P<0.05). Correlation analysis between p53, PGP9.5, SOX2, GAGE7, GBU4-5 and MAGE A1 proteins in tumor tissues and serum showed that the positive rates and gene expression levels of P53, PGP9.5, SOX2, GAGE7, GBU4-5 and MAGE A1 were correlated with TNM stage and differentiation level (P<0.05). There was no significant difference in gender, age, tumor diameter and pathological type (P>0.05). The protein expression levels of p53, PGP9.5, SOX2, GAGE7, GBU4-5 and MAGE A1 in tumor tissues were consistent with their expression levels in peripheral blood (P>0.05). Conclusion: Positive expressions of p53, PGP9.5, SOX2, GAGE7, GBU4-5 and MAGE A1 protein in lung cancer tissues and peripheral serum were closely related to TNM staging and differentiation.http://www.china-oncology.com/fileup/1007-3639/PDF/1676258276072-2126946475.pdf|lung cancer|tumor suppressor gene (p53)|protein gene product (pgp9.5)|transcription factor (sox2)|tumor-associated gene protein (gage7)|helicase (gbu4-5)|melanoma antigen (mage a1) |
| spellingShingle | ZOU Chunyuan, XU Xiaofeng, LU Renquan, GUO Lin Detection of p53, PGP9.5, SOX2, GAGE7, GBU4-5 and MAGE A1 protein levels in lung cancer tissues and peripheral blood and their clinical value Zhongguo aizheng zazhi |lung cancer|tumor suppressor gene (p53)|protein gene product (pgp9.5)|transcription factor (sox2)|tumor-associated gene protein (gage7)|helicase (gbu4-5)|melanoma antigen (mage a1) |
| title | Detection of p53, PGP9.5, SOX2, GAGE7, GBU4-5 and MAGE A1 protein levels in lung cancer tissues and peripheral blood and their clinical value |
| title_full | Detection of p53, PGP9.5, SOX2, GAGE7, GBU4-5 and MAGE A1 protein levels in lung cancer tissues and peripheral blood and their clinical value |
| title_fullStr | Detection of p53, PGP9.5, SOX2, GAGE7, GBU4-5 and MAGE A1 protein levels in lung cancer tissues and peripheral blood and their clinical value |
| title_full_unstemmed | Detection of p53, PGP9.5, SOX2, GAGE7, GBU4-5 and MAGE A1 protein levels in lung cancer tissues and peripheral blood and their clinical value |
| title_short | Detection of p53, PGP9.5, SOX2, GAGE7, GBU4-5 and MAGE A1 protein levels in lung cancer tissues and peripheral blood and their clinical value |
| title_sort | detection of p53 pgp9 5 sox2 gage7 gbu4 5 and mage a1 protein levels in lung cancer tissues and peripheral blood and their clinical value |
| topic | |lung cancer|tumor suppressor gene (p53)|protein gene product (pgp9.5)|transcription factor (sox2)|tumor-associated gene protein (gage7)|helicase (gbu4-5)|melanoma antigen (mage a1) |
| url | http://www.china-oncology.com/fileup/1007-3639/PDF/1676258276072-2126946475.pdf |
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