Restriction fragment length polymorphisms of mitochondrial DNA among five freshwater fish species of the genus Astyanax (Pisces, Characidae)
Restriction fragment length polymorphism (RFLP) analysis of mitochondrial DNA (mtDNA) was employed to characterize species and populations of Astyanax, a Neotropical freshwater fish genus. Samples of five species, A. altiparanae, A. fasciatus, A. lacustris, A. scabripinnis paranae and A. schubarti,...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sociedade Brasileira de Genética
2002-01-01
|
| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400008 |
| _version_ | 1828771635255050240 |
|---|---|
| author | Cinthia Bachir Moysés Lurdes F. de Almeida-Toledo |
| author_facet | Cinthia Bachir Moysés Lurdes F. de Almeida-Toledo |
| author_sort | Cinthia Bachir Moysés |
| collection | DOAJ |
| description | Restriction fragment length polymorphism (RFLP) analysis of mitochondrial DNA (mtDNA) was employed to characterize species and populations of Astyanax, a Neotropical freshwater fish genus. Samples of five species, A. altiparanae, A. fasciatus, A. lacustris, A. scabripinnis paranae and A. schubarti, from the Upper Paraná and São Francisco river basins were analyzed. Two out of the ten restriction enzymes employed generated species-specific mtDNA patterns for each of the five species. MtDNA exhibited considerable polymorphism within and among populations. All populations sampled showed relatively high values of haplotype diversity. Geographically localized haplotypes were detected for A. altiparanae and A. fasciatus from the Upper Paraná and São Francisco basins. The relationships between populations are discussed. |
| first_indexed | 2024-12-11T14:28:43Z |
| format | Article |
| id | doaj.art-8ee0a6e65e2c4bac860d608214259270 |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-11T14:28:43Z |
| publishDate | 2002-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-8ee0a6e65e2c4bac860d6082142592702022-12-22T01:02:31ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852002-01-0125440140710.1590/S1415-47572002000400008Restriction fragment length polymorphisms of mitochondrial DNA among five freshwater fish species of the genus Astyanax (Pisces, Characidae)Cinthia Bachir MoysésLurdes F. de Almeida-ToledoRestriction fragment length polymorphism (RFLP) analysis of mitochondrial DNA (mtDNA) was employed to characterize species and populations of Astyanax, a Neotropical freshwater fish genus. Samples of five species, A. altiparanae, A. fasciatus, A. lacustris, A. scabripinnis paranae and A. schubarti, from the Upper Paraná and São Francisco river basins were analyzed. Two out of the ten restriction enzymes employed generated species-specific mtDNA patterns for each of the five species. MtDNA exhibited considerable polymorphism within and among populations. All populations sampled showed relatively high values of haplotype diversity. Geographically localized haplotypes were detected for A. altiparanae and A. fasciatus from the Upper Paraná and São Francisco basins. The relationships between populations are discussed.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400008mtDNARFLPAstyanax |
| spellingShingle | Cinthia Bachir Moysés Lurdes F. de Almeida-Toledo Restriction fragment length polymorphisms of mitochondrial DNA among five freshwater fish species of the genus Astyanax (Pisces, Characidae) Genetics and Molecular Biology mtDNA RFLP Astyanax |
| title | Restriction fragment length polymorphisms of mitochondrial DNA among five freshwater fish species of the genus Astyanax (Pisces, Characidae) |
| title_full | Restriction fragment length polymorphisms of mitochondrial DNA among five freshwater fish species of the genus Astyanax (Pisces, Characidae) |
| title_fullStr | Restriction fragment length polymorphisms of mitochondrial DNA among five freshwater fish species of the genus Astyanax (Pisces, Characidae) |
| title_full_unstemmed | Restriction fragment length polymorphisms of mitochondrial DNA among five freshwater fish species of the genus Astyanax (Pisces, Characidae) |
| title_short | Restriction fragment length polymorphisms of mitochondrial DNA among five freshwater fish species of the genus Astyanax (Pisces, Characidae) |
| title_sort | restriction fragment length polymorphisms of mitochondrial dna among five freshwater fish species of the genus astyanax pisces characidae |
| topic | mtDNA RFLP Astyanax |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400008 |
| work_keys_str_mv | AT cinthiabachirmoyses restrictionfragmentlengthpolymorphismsofmitochondrialdnaamongfivefreshwaterfishspeciesofthegenusastyanaxpiscescharacidae AT lurdesfdealmeidatoledo restrictionfragmentlengthpolymorphismsofmitochondrialdnaamongfivefreshwaterfishspeciesofthegenusastyanaxpiscescharacidae |