The Axenic and Gnotobiotic Mosquito: Emerging Models for Microbiome Host Interactions
The increasing availability of modern research tools has enabled a revolution in studies of non-model organisms. Yet, one aspect that remains difficult or impossible to control in many model and most non-model organisms is the presence and composition of the host-associated microbiota or the microbi...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-07-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.714222/full |
| _version_ | 1818883581448028160 |
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| author | Blaire Steven Josephine Hyde Jacquelyn C. LaReau Doug E. Brackney Doug E. Brackney |
| author_facet | Blaire Steven Josephine Hyde Jacquelyn C. LaReau Doug E. Brackney Doug E. Brackney |
| author_sort | Blaire Steven |
| collection | DOAJ |
| description | The increasing availability of modern research tools has enabled a revolution in studies of non-model organisms. Yet, one aspect that remains difficult or impossible to control in many model and most non-model organisms is the presence and composition of the host-associated microbiota or the microbiome. In this review, we explore the development of axenic (microbe-free) mosquito models and what these systems reveal about the role of the microbiome in mosquito biology. Additionally, the axenic host is a blank template on which a microbiome of known composition can be introduced, also known as a gnotobiotic organism. Finally, we identify a “most wanted” list of common mosquito microbiome members that show the greatest potential to influence host phenotypes. We propose that these are high-value targets to be employed in future gnotobiotic studies. The use of axenic and gnotobiotic organisms will transition the microbiome into another experimental variable that can be manipulated and controlled. Through these efforts, the mosquito will be a true model for examining host microbiome interactions. |
| first_indexed | 2024-12-19T15:35:56Z |
| format | Article |
| id | doaj.art-8ee464e670814a53afbd8175d5beed6c |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-19T15:35:56Z |
| publishDate | 2021-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-8ee464e670814a53afbd8175d5beed6c2022-12-21T20:15:36ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-07-011210.3389/fmicb.2021.714222714222The Axenic and Gnotobiotic Mosquito: Emerging Models for Microbiome Host InteractionsBlaire Steven0Josephine Hyde1Jacquelyn C. LaReau2Doug E. Brackney3Doug E. Brackney4Department of Environmental Sciences, Connecticut Agricultural Experiment Station, New Haven, CT, United StatesDepartment of Environmental Sciences, Connecticut Agricultural Experiment Station, New Haven, CT, United StatesDepartment of Environmental Sciences, Connecticut Agricultural Experiment Station, New Haven, CT, United StatesDepartment of Environmental Sciences, Connecticut Agricultural Experiment Station, New Haven, CT, United StatesCenter for Vector Biology and Zoonotic Diseases, Connecticut Agricultural Experiment Station, New Haven, CT, United StatesThe increasing availability of modern research tools has enabled a revolution in studies of non-model organisms. Yet, one aspect that remains difficult or impossible to control in many model and most non-model organisms is the presence and composition of the host-associated microbiota or the microbiome. In this review, we explore the development of axenic (microbe-free) mosquito models and what these systems reveal about the role of the microbiome in mosquito biology. Additionally, the axenic host is a blank template on which a microbiome of known composition can be introduced, also known as a gnotobiotic organism. Finally, we identify a “most wanted” list of common mosquito microbiome members that show the greatest potential to influence host phenotypes. We propose that these are high-value targets to be employed in future gnotobiotic studies. The use of axenic and gnotobiotic organisms will transition the microbiome into another experimental variable that can be manipulated and controlled. Through these efforts, the mosquito will be a true model for examining host microbiome interactions.https://www.frontiersin.org/articles/10.3389/fmicb.2021.714222/fullaxenicgnotobioticmosquitomicrobiomemodel system |
| spellingShingle | Blaire Steven Josephine Hyde Jacquelyn C. LaReau Doug E. Brackney Doug E. Brackney The Axenic and Gnotobiotic Mosquito: Emerging Models for Microbiome Host Interactions Frontiers in Microbiology axenic gnotobiotic mosquito microbiome model system |
| title | The Axenic and Gnotobiotic Mosquito: Emerging Models for Microbiome Host Interactions |
| title_full | The Axenic and Gnotobiotic Mosquito: Emerging Models for Microbiome Host Interactions |
| title_fullStr | The Axenic and Gnotobiotic Mosquito: Emerging Models for Microbiome Host Interactions |
| title_full_unstemmed | The Axenic and Gnotobiotic Mosquito: Emerging Models for Microbiome Host Interactions |
| title_short | The Axenic and Gnotobiotic Mosquito: Emerging Models for Microbiome Host Interactions |
| title_sort | axenic and gnotobiotic mosquito emerging models for microbiome host interactions |
| topic | axenic gnotobiotic mosquito microbiome model system |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.714222/full |
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