miR-338-3p Is Regulated by Estrogens through GPER in Breast Cancer Cells and Cancer-Associated Fibroblasts (CAFs)
Estrogens acting through the classic estrogen receptors (ERs) and the G protein estrogen receptor (GPER) regulate the expression of diverse miRNAs, small sequences of non-coding RNA involved in several pathophysiological conditions, including breast cancer. In order to provide novel insights on miRN...
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MDPI AG
2018-11-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/7/11/203 |
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| author | Adele Vivacqua Anna Sebastiani Anna Maria Miglietta Damiano Cosimo Rigiracciolo Francesca Cirillo Giulia Raffaella Galli Marianna Talia Maria Francesca Santolla Rosamaria Lappano Francesca Giordano Maria Luisa Panno Marcello Maggiolini |
| author_facet | Adele Vivacqua Anna Sebastiani Anna Maria Miglietta Damiano Cosimo Rigiracciolo Francesca Cirillo Giulia Raffaella Galli Marianna Talia Maria Francesca Santolla Rosamaria Lappano Francesca Giordano Maria Luisa Panno Marcello Maggiolini |
| author_sort | Adele Vivacqua |
| collection | DOAJ |
| description | Estrogens acting through the classic estrogen receptors (ERs) and the G protein estrogen receptor (GPER) regulate the expression of diverse miRNAs, small sequences of non-coding RNA involved in several pathophysiological conditions, including breast cancer. In order to provide novel insights on miRNAs regulation by estrogens in breast tumor, we evaluated the expression of 754 miRNAs by TaqMan Array in ER-negative and GPER-positive SkBr3 breast cancer cells and cancer-associated fibroblasts (CAFs) upon 17β-estradiol (E2) treatment. Various miRNAs were regulated by E2 in a peculiar manner in SkBr3 cancer cells and CAFs, while miR-338-3p displayed a similar regulation in both cell types. By METABRIC database analysis we ascertained that miR-338-3p positively correlates with overall survival in breast cancer patients, according to previous studies showing that miR-338-3p may suppress the growth and invasion of different cancer cells. Well-fitting with these data, a miR-338-3p mimic sequence decreased and a miR-338-3p inhibitor sequence rescued the expression of genes and the proliferative effects induced by E2 through GPER in SkBr3 cancer cells and CAFs. Altogether, our results provide novel evidence on the molecular mechanisms by which E2 may regulate miR-338-3p toward breast cancer progression. |
| first_indexed | 2024-03-12T06:14:53Z |
| format | Article |
| id | doaj.art-8ee68662a27c4155a5a3a037f01be8b3 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T06:14:53Z |
| publishDate | 2018-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-8ee68662a27c4155a5a3a037f01be8b32023-09-03T02:41:18ZengMDPI AGCells2073-44092018-11-0171120310.3390/cells7110203cells7110203miR-338-3p Is Regulated by Estrogens through GPER in Breast Cancer Cells and Cancer-Associated Fibroblasts (CAFs)Adele Vivacqua0Anna Sebastiani1Anna Maria Miglietta2Damiano Cosimo Rigiracciolo3Francesca Cirillo4Giulia Raffaella Galli5Marianna Talia6Maria Francesca Santolla7Rosamaria Lappano8Francesca Giordano9Maria Luisa Panno10Marcello Maggiolini11Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, ItalyDepartment of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, ItalyRegional HospitalCosenza, 87100 Cosenza, ItalyDepartment of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, ItalyDepartment of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, ItalyDepartment of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, ItalyDepartment of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, ItalyDepartment of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, ItalyDepartment of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, ItalyDepartment of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, ItalyDepartment of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, ItalyDepartment of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, ItalyEstrogens acting through the classic estrogen receptors (ERs) and the G protein estrogen receptor (GPER) regulate the expression of diverse miRNAs, small sequences of non-coding RNA involved in several pathophysiological conditions, including breast cancer. In order to provide novel insights on miRNAs regulation by estrogens in breast tumor, we evaluated the expression of 754 miRNAs by TaqMan Array in ER-negative and GPER-positive SkBr3 breast cancer cells and cancer-associated fibroblasts (CAFs) upon 17β-estradiol (E2) treatment. Various miRNAs were regulated by E2 in a peculiar manner in SkBr3 cancer cells and CAFs, while miR-338-3p displayed a similar regulation in both cell types. By METABRIC database analysis we ascertained that miR-338-3p positively correlates with overall survival in breast cancer patients, according to previous studies showing that miR-338-3p may suppress the growth and invasion of different cancer cells. Well-fitting with these data, a miR-338-3p mimic sequence decreased and a miR-338-3p inhibitor sequence rescued the expression of genes and the proliferative effects induced by E2 through GPER in SkBr3 cancer cells and CAFs. Altogether, our results provide novel evidence on the molecular mechanisms by which E2 may regulate miR-338-3p toward breast cancer progression.https://www.mdpi.com/2073-4409/7/11/203breast cancerCAFsestrogensGPERmiR-338-3pc-FosCyclin D1 |
| spellingShingle | Adele Vivacqua Anna Sebastiani Anna Maria Miglietta Damiano Cosimo Rigiracciolo Francesca Cirillo Giulia Raffaella Galli Marianna Talia Maria Francesca Santolla Rosamaria Lappano Francesca Giordano Maria Luisa Panno Marcello Maggiolini miR-338-3p Is Regulated by Estrogens through GPER in Breast Cancer Cells and Cancer-Associated Fibroblasts (CAFs) Cells breast cancer CAFs estrogens GPER miR-338-3p c-Fos Cyclin D1 |
| title | miR-338-3p Is Regulated by Estrogens through GPER in Breast Cancer Cells and Cancer-Associated Fibroblasts (CAFs) |
| title_full | miR-338-3p Is Regulated by Estrogens through GPER in Breast Cancer Cells and Cancer-Associated Fibroblasts (CAFs) |
| title_fullStr | miR-338-3p Is Regulated by Estrogens through GPER in Breast Cancer Cells and Cancer-Associated Fibroblasts (CAFs) |
| title_full_unstemmed | miR-338-3p Is Regulated by Estrogens through GPER in Breast Cancer Cells and Cancer-Associated Fibroblasts (CAFs) |
| title_short | miR-338-3p Is Regulated by Estrogens through GPER in Breast Cancer Cells and Cancer-Associated Fibroblasts (CAFs) |
| title_sort | mir 338 3p is regulated by estrogens through gper in breast cancer cells and cancer associated fibroblasts cafs |
| topic | breast cancer CAFs estrogens GPER miR-338-3p c-Fos Cyclin D1 |
| url | https://www.mdpi.com/2073-4409/7/11/203 |
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