Distribution of Polyphenolic and Isoprenoid Compounds and Biological Activity Differences between in the Fruit Skin + Pulp, Seeds, and Leaves of New Biotypes of <i>Elaeagnus</i><i>multiflora</i> Thunb
The purpose of this study was to determine the distribution of polyphenolic and isoprenoid compounds and organic acids in the fruit skin + pulp, seeds, and leaves of six new biotypes of <i>Elaeagnus multiflora</i> Thunb., as well as their in vitro biological potency. The polyphenols and...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/6/849 |
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| author | Sabina Lachowicz-Wiśniewska Ireneusz Kapusta Carla M. Stinco Antonio J. Meléndez-Martínez Anna Bieniek Ireneusz Ochmian Zygmunt Gil |
| author_facet | Sabina Lachowicz-Wiśniewska Ireneusz Kapusta Carla M. Stinco Antonio J. Meléndez-Martínez Anna Bieniek Ireneusz Ochmian Zygmunt Gil |
| author_sort | Sabina Lachowicz-Wiśniewska |
| collection | DOAJ |
| description | The purpose of this study was to determine the distribution of polyphenolic and isoprenoid compounds and organic acids in the fruit skin + pulp, seeds, and leaves of six new biotypes of <i>Elaeagnus multiflora</i> Thunb., as well as their in vitro biological potency. The polyphenols and isoprenoids were determined with UPLC-PDA-MS/MS (ultra-performance liquid chromatography coupled to photodiode array detection and electrospray ionization tandem mass spectrometry) and RRLC-MS/MS (rapid resolution liquid chromatography/tandem mass spectrometry) methods, the organic acid with HPLC-RID (high-performance liquid chromatography coupled to a Refractive Index Detector), and the antioxidant capacity using ABTS and FRAP assays. Enzymatic activity was established as the ability to inhibit α-amylase, α-glucosidase, and pancreatic lipase. Owing to such an effective technique, 88 compounds were recorded, with 17 polyphenolic compounds and 3 isoprenoids identified for the first time in the seeds and leaves of cherry silverberry. In total, 55 compounds were identified in the leaves, 36 in the seeds, and 31 in the fruit skin + pulp. The predominant polyphenol was polymeric procyanidin (66–95% of total polyphenolics), whereas the predominant isoprenoids were chlorophyll b and (all-E)-lycopene. The results of our work noted that there are significant differences in the profiles of several secondary metabolites between the analyzed parts of the plant, and depending on the need, the compounds can be used to develop different innovative food or cosmetic products. |
| first_indexed | 2024-03-10T11:02:35Z |
| format | Article |
| id | doaj.art-8ee91bb22f8c40b48e7d09a2c9f7784a |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T11:02:35Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-8ee91bb22f8c40b48e7d09a2c9f7784a2023-11-21T21:26:04ZengMDPI AGAntioxidants2076-39212021-05-0110684910.3390/antiox10060849Distribution of Polyphenolic and Isoprenoid Compounds and Biological Activity Differences between in the Fruit Skin + Pulp, Seeds, and Leaves of New Biotypes of <i>Elaeagnus</i><i>multiflora</i> ThunbSabina Lachowicz-Wiśniewska0Ireneusz Kapusta1Carla M. Stinco2Antonio J. Meléndez-Martínez3Anna Bieniek4Ireneusz Ochmian5Zygmunt Gil6Department of Fermentation and Cereals Technology, Wrocław University of Environmental and Life Science, Chełmońskiego 37, 51-630 Wroclaw, PolandDepartment of Food Technology and Human Nutrition, Faculty of Biology and Agriculture, Rzeszow University, Zelwerowicza 4, 35-601 Rzeszow, PolandFood Colour and Quality Laboratory, Area of Nutrition and Food Science, Universidad de Sevilla, 41012 Seville, SpainFood Colour and Quality Laboratory, Area of Nutrition and Food Science, Universidad de Sevilla, 41012 Seville, SpainDepartment of Horticulture, University of Warmia and Mazury, Prawocheńskiego 21, 10-720 Olsztyn, PolandDepartment of Horticulture, West Pomeranian University of Technology in Szczecin, Słowackiego 17, 71-434 Szczecin, PolandDepartment of Fermentation and Cereals Technology, Wrocław University of Environmental and Life Science, Chełmońskiego 37, 51-630 Wroclaw, PolandThe purpose of this study was to determine the distribution of polyphenolic and isoprenoid compounds and organic acids in the fruit skin + pulp, seeds, and leaves of six new biotypes of <i>Elaeagnus multiflora</i> Thunb., as well as their in vitro biological potency. The polyphenols and isoprenoids were determined with UPLC-PDA-MS/MS (ultra-performance liquid chromatography coupled to photodiode array detection and electrospray ionization tandem mass spectrometry) and RRLC-MS/MS (rapid resolution liquid chromatography/tandem mass spectrometry) methods, the organic acid with HPLC-RID (high-performance liquid chromatography coupled to a Refractive Index Detector), and the antioxidant capacity using ABTS and FRAP assays. Enzymatic activity was established as the ability to inhibit α-amylase, α-glucosidase, and pancreatic lipase. Owing to such an effective technique, 88 compounds were recorded, with 17 polyphenolic compounds and 3 isoprenoids identified for the first time in the seeds and leaves of cherry silverberry. In total, 55 compounds were identified in the leaves, 36 in the seeds, and 31 in the fruit skin + pulp. The predominant polyphenol was polymeric procyanidin (66–95% of total polyphenolics), whereas the predominant isoprenoids were chlorophyll b and (all-E)-lycopene. The results of our work noted that there are significant differences in the profiles of several secondary metabolites between the analyzed parts of the plant, and depending on the need, the compounds can be used to develop different innovative food or cosmetic products.https://www.mdpi.com/2076-3921/10/6/849carotenoidscherry silverberryseeds, polyphenolic compoundsantioxidative capacityanti-diabetic activityorganic acids |
| spellingShingle | Sabina Lachowicz-Wiśniewska Ireneusz Kapusta Carla M. Stinco Antonio J. Meléndez-Martínez Anna Bieniek Ireneusz Ochmian Zygmunt Gil Distribution of Polyphenolic and Isoprenoid Compounds and Biological Activity Differences between in the Fruit Skin + Pulp, Seeds, and Leaves of New Biotypes of <i>Elaeagnus</i><i>multiflora</i> Thunb Antioxidants carotenoids cherry silverberry seeds, polyphenolic compounds antioxidative capacity anti-diabetic activity organic acids |
| title | Distribution of Polyphenolic and Isoprenoid Compounds and Biological Activity Differences between in the Fruit Skin + Pulp, Seeds, and Leaves of New Biotypes of <i>Elaeagnus</i><i>multiflora</i> Thunb |
| title_full | Distribution of Polyphenolic and Isoprenoid Compounds and Biological Activity Differences between in the Fruit Skin + Pulp, Seeds, and Leaves of New Biotypes of <i>Elaeagnus</i><i>multiflora</i> Thunb |
| title_fullStr | Distribution of Polyphenolic and Isoprenoid Compounds and Biological Activity Differences between in the Fruit Skin + Pulp, Seeds, and Leaves of New Biotypes of <i>Elaeagnus</i><i>multiflora</i> Thunb |
| title_full_unstemmed | Distribution of Polyphenolic and Isoprenoid Compounds and Biological Activity Differences between in the Fruit Skin + Pulp, Seeds, and Leaves of New Biotypes of <i>Elaeagnus</i><i>multiflora</i> Thunb |
| title_short | Distribution of Polyphenolic and Isoprenoid Compounds and Biological Activity Differences between in the Fruit Skin + Pulp, Seeds, and Leaves of New Biotypes of <i>Elaeagnus</i><i>multiflora</i> Thunb |
| title_sort | distribution of polyphenolic and isoprenoid compounds and biological activity differences between in the fruit skin pulp seeds and leaves of new biotypes of i elaeagnus i i multiflora i thunb |
| topic | carotenoids cherry silverberry seeds, polyphenolic compounds antioxidative capacity anti-diabetic activity organic acids |
| url | https://www.mdpi.com/2076-3921/10/6/849 |
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