Molecular Basis of Resistance to Mesosulfuron-Methyl in a Black-Grass (<i>Alopecurus myosuroides</i> Huds.) Population from China
Black-grass (<i>Alopecurus myosuroides</i> Huds.) is a common weed in Chinese wheat fields, and has become troublesome due to its evolution of herbicide resistance. One black-grass population (HN-14) collected from a wheat field where herbicides were applied was suspected to be resistant...
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MDPI AG
2022-09-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/9/2203 |
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| author | Xiangyang Qin Cheng Yang Mengmeng Hu Yunxia Duan Na Zhang Jinxin Wang Hengzhi Wang Weitang Liu |
| author_facet | Xiangyang Qin Cheng Yang Mengmeng Hu Yunxia Duan Na Zhang Jinxin Wang Hengzhi Wang Weitang Liu |
| author_sort | Xiangyang Qin |
| collection | DOAJ |
| description | Black-grass (<i>Alopecurus myosuroides</i> Huds.) is a common weed in Chinese wheat fields, and has become troublesome due to its evolution of herbicide resistance. One black-grass population (HN-14) collected from a wheat field where herbicides were applied was suspected to be resistant (R) to mesosulfuron-methyl. This study aims to establish a cross-resistance pattern and explore potential resistance mechanisms. The results of a whole-plant dose response assay showed that the resistant (R) population had a high of resistance to mesosulfuron-methyl (33-fold); meanwhile, no synergism of P450s activity inhibitor malathion was observed. The sequencing results revealed that ALS resistance mutation Trp-574-Leu occurred in R plants. The results of in vitro ALS enzyme activity assays also supported that the extractable ALS from R plants were 23.22-fold resistant to mesosulfuron-methyl. In the light of the “R” resistance rating system, HN-14 has evolved RRR and RR resistance to fenoxaprop-P-ethyl, clodinafop-propargyl, haloxyfop-methyl, and fluazifop-P-butyl and R? (resistance may be developing) to pinoxaden, however remains sensitive to imazethapyr, quizalofop-P-ethyl, tralkoxydim, and isoproturon. These results indicated that the mesosulfuron-methyl resistance in the black-grass population HN-14 was driven by a target-site mechanism rather than a nontarget (at least P450s-mediated) mechanism. |
| first_indexed | 2024-03-10T00:58:50Z |
| format | Article |
| id | doaj.art-8ee98164ff4f49d99e69041bf02d231b |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T00:58:50Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-8ee98164ff4f49d99e69041bf02d231b2023-11-23T14:39:04ZengMDPI AGAgronomy2073-43952022-09-01129220310.3390/agronomy12092203Molecular Basis of Resistance to Mesosulfuron-Methyl in a Black-Grass (<i>Alopecurus myosuroides</i> Huds.) Population from ChinaXiangyang Qin0Cheng Yang1Mengmeng Hu2Yunxia Duan3Na Zhang4Jinxin Wang5Hengzhi Wang6Weitang Liu7College of Plant Protection, Shandong Agricultural University, Tai’an 271018, ChinaCollege of Plant Protection, Shandong Agricultural University, Tai’an 271018, ChinaCollege of Plant Protection, Shandong Agricultural University, Tai’an 271018, ChinaCollege of Plant Protection, Shandong Agricultural University, Tai’an 271018, ChinaResearch Center of Pesticide Environmental Toxicology, Shandong Agricultural University, Tai’an 271018, ChinaCollege of Plant Protection, Shandong Agricultural University, Tai’an 271018, ChinaCollege of Plant Protection, Shandong Agricultural University, Tai’an 271018, ChinaCollege of Plant Protection, Shandong Agricultural University, Tai’an 271018, ChinaBlack-grass (<i>Alopecurus myosuroides</i> Huds.) is a common weed in Chinese wheat fields, and has become troublesome due to its evolution of herbicide resistance. One black-grass population (HN-14) collected from a wheat field where herbicides were applied was suspected to be resistant (R) to mesosulfuron-methyl. This study aims to establish a cross-resistance pattern and explore potential resistance mechanisms. The results of a whole-plant dose response assay showed that the resistant (R) population had a high of resistance to mesosulfuron-methyl (33-fold); meanwhile, no synergism of P450s activity inhibitor malathion was observed. The sequencing results revealed that ALS resistance mutation Trp-574-Leu occurred in R plants. The results of in vitro ALS enzyme activity assays also supported that the extractable ALS from R plants were 23.22-fold resistant to mesosulfuron-methyl. In the light of the “R” resistance rating system, HN-14 has evolved RRR and RR resistance to fenoxaprop-P-ethyl, clodinafop-propargyl, haloxyfop-methyl, and fluazifop-P-butyl and R? (resistance may be developing) to pinoxaden, however remains sensitive to imazethapyr, quizalofop-P-ethyl, tralkoxydim, and isoproturon. These results indicated that the mesosulfuron-methyl resistance in the black-grass population HN-14 was driven by a target-site mechanism rather than a nontarget (at least P450s-mediated) mechanism.https://www.mdpi.com/2073-4395/12/9/2203herbicide resistance mechanismcross resistanceacetolactate synthase (ALS) inhibitor<i>Alopecurus myosuroides</i> huds |
| spellingShingle | Xiangyang Qin Cheng Yang Mengmeng Hu Yunxia Duan Na Zhang Jinxin Wang Hengzhi Wang Weitang Liu Molecular Basis of Resistance to Mesosulfuron-Methyl in a Black-Grass (<i>Alopecurus myosuroides</i> Huds.) Population from China Agronomy herbicide resistance mechanism cross resistance acetolactate synthase (ALS) inhibitor <i>Alopecurus myosuroides</i> huds |
| title | Molecular Basis of Resistance to Mesosulfuron-Methyl in a Black-Grass (<i>Alopecurus myosuroides</i> Huds.) Population from China |
| title_full | Molecular Basis of Resistance to Mesosulfuron-Methyl in a Black-Grass (<i>Alopecurus myosuroides</i> Huds.) Population from China |
| title_fullStr | Molecular Basis of Resistance to Mesosulfuron-Methyl in a Black-Grass (<i>Alopecurus myosuroides</i> Huds.) Population from China |
| title_full_unstemmed | Molecular Basis of Resistance to Mesosulfuron-Methyl in a Black-Grass (<i>Alopecurus myosuroides</i> Huds.) Population from China |
| title_short | Molecular Basis of Resistance to Mesosulfuron-Methyl in a Black-Grass (<i>Alopecurus myosuroides</i> Huds.) Population from China |
| title_sort | molecular basis of resistance to mesosulfuron methyl in a black grass i alopecurus myosuroides i huds population from china |
| topic | herbicide resistance mechanism cross resistance acetolactate synthase (ALS) inhibitor <i>Alopecurus myosuroides</i> huds |
| url | https://www.mdpi.com/2073-4395/12/9/2203 |
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