Restoring the ON Switch in Blind Retinas: Opto-mGluR6, a Next-Generation, Cell-Tailored Optogenetic Tool.
Photoreceptor degeneration is one of the most prevalent causes of blindness. Despite photoreceptor loss, the inner retina and central visual pathways remain intact over an extended time period, which has led to creative optogenetic approaches to restore light sensitivity in the surviving inner retin...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-05-01
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| Series: | PLoS Biology |
| Online Access: | http://europepmc.org/articles/PMC4423780?pdf=render |
| _version_ | 1819008427351867392 |
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| author | Michiel van Wyk Justyna Pielecka-Fortuna Siegrid Löwel Sonja Kleinlogel |
| author_facet | Michiel van Wyk Justyna Pielecka-Fortuna Siegrid Löwel Sonja Kleinlogel |
| author_sort | Michiel van Wyk |
| collection | DOAJ |
| description | Photoreceptor degeneration is one of the most prevalent causes of blindness. Despite photoreceptor loss, the inner retina and central visual pathways remain intact over an extended time period, which has led to creative optogenetic approaches to restore light sensitivity in the surviving inner retina. The major drawbacks of all optogenetic tools recently developed and tested in mouse models are their low light sensitivity and lack of physiological compatibility. Here we introduce a next-generation optogenetic tool, Opto-mGluR6, designed for retinal ON-bipolar cells, which overcomes these limitations. We show that Opto-mGluR6, a chimeric protein consisting of the intracellular domains of the ON-bipolar cell-specific metabotropic glutamate receptor mGluR6 and the light-sensing domains of melanopsin, reliably recovers vision at the retinal, cortical, and behavioral levels under moderate daylight illumination. |
| first_indexed | 2024-12-21T00:40:18Z |
| format | Article |
| id | doaj.art-8eee7d7acf1c4f8fa69144338d610c90 |
| institution | Directory Open Access Journal |
| issn | 1544-9173 1545-7885 |
| language | English |
| last_indexed | 2024-12-21T00:40:18Z |
| publishDate | 2015-05-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj.art-8eee7d7acf1c4f8fa69144338d610c902022-12-21T19:21:43ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852015-05-01135e100214310.1371/journal.pbio.1002143Restoring the ON Switch in Blind Retinas: Opto-mGluR6, a Next-Generation, Cell-Tailored Optogenetic Tool.Michiel van WykJustyna Pielecka-FortunaSiegrid LöwelSonja KleinlogelPhotoreceptor degeneration is one of the most prevalent causes of blindness. Despite photoreceptor loss, the inner retina and central visual pathways remain intact over an extended time period, which has led to creative optogenetic approaches to restore light sensitivity in the surviving inner retina. The major drawbacks of all optogenetic tools recently developed and tested in mouse models are their low light sensitivity and lack of physiological compatibility. Here we introduce a next-generation optogenetic tool, Opto-mGluR6, designed for retinal ON-bipolar cells, which overcomes these limitations. We show that Opto-mGluR6, a chimeric protein consisting of the intracellular domains of the ON-bipolar cell-specific metabotropic glutamate receptor mGluR6 and the light-sensing domains of melanopsin, reliably recovers vision at the retinal, cortical, and behavioral levels under moderate daylight illumination.http://europepmc.org/articles/PMC4423780?pdf=render |
| spellingShingle | Michiel van Wyk Justyna Pielecka-Fortuna Siegrid Löwel Sonja Kleinlogel Restoring the ON Switch in Blind Retinas: Opto-mGluR6, a Next-Generation, Cell-Tailored Optogenetic Tool. PLoS Biology |
| title | Restoring the ON Switch in Blind Retinas: Opto-mGluR6, a Next-Generation, Cell-Tailored Optogenetic Tool. |
| title_full | Restoring the ON Switch in Blind Retinas: Opto-mGluR6, a Next-Generation, Cell-Tailored Optogenetic Tool. |
| title_fullStr | Restoring the ON Switch in Blind Retinas: Opto-mGluR6, a Next-Generation, Cell-Tailored Optogenetic Tool. |
| title_full_unstemmed | Restoring the ON Switch in Blind Retinas: Opto-mGluR6, a Next-Generation, Cell-Tailored Optogenetic Tool. |
| title_short | Restoring the ON Switch in Blind Retinas: Opto-mGluR6, a Next-Generation, Cell-Tailored Optogenetic Tool. |
| title_sort | restoring the on switch in blind retinas opto mglur6 a next generation cell tailored optogenetic tool |
| url | http://europepmc.org/articles/PMC4423780?pdf=render |
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