Simultaneous Saccharification and Fermentation of Pine Sawdust (Pinus pseudostrobus L.) Pretreated with Nitric Acid and Sodium Hydroxide for Bioethanol Production
With the purpose of taking advantage of pine sawdust residue coming from a sawmill located in Michoacán, México, a pretreatment with nitric acid and sodium hydroxide was performed. Also, the production of bioethanol by enzymatic hydrolysis was investigated. Using a response surface method, the inter...
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| Format: | Article |
| Language: | English |
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North Carolina State University
2016-12-01
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| Series: | BioResources |
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| Online Access: | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_12_1_1052_Farias_Sanchez_Saccharification_Fermentation_Pine_Sawdust |
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| author | Juan Carlos Farías-Sánchez Ulises Velázquez-Valadez María Guadalupe Pineda-Pimentel Javier López-Miranda Agustín Jaime Castro-Montoya Artemio Carrillo-Parra Alfonso Vargas-Santillán José Guadalupe Rutiaga-Quiñones |
| author_facet | Juan Carlos Farías-Sánchez Ulises Velázquez-Valadez María Guadalupe Pineda-Pimentel Javier López-Miranda Agustín Jaime Castro-Montoya Artemio Carrillo-Parra Alfonso Vargas-Santillán José Guadalupe Rutiaga-Quiñones |
| author_sort | Juan Carlos Farías-Sánchez |
| collection | DOAJ |
| description | With the purpose of taking advantage of pine sawdust residue coming from a sawmill located in Michoacán, México, a pretreatment with nitric acid and sodium hydroxide was performed. Also, the production of bioethanol by enzymatic hydrolysis was investigated. Using a response surface method, the intermediate points for the optimal HNO3 concentration were determined. Results showed that using HNO3 as a pretreatment leads to higher ethanol yields at an optimal concentration of 10.90% HNO3. After a 30-min pretreatment with 10.90% HNO3 at 114.32 °C, followed by 1% NaOH and enzymatic hydrolysis performed in shaker at a pH of 4.8 and 150 rpm for 72 h, with an enzyme loading of 25 FPU/g of total carbohydrates, the reducing sugars concentration was 99.2% (conversion of polysaccharides to monomers). On the other hand, the ethanol yield obtained from the simultaneous saccharification and fermentation treatment was 15.0 g/L, and the separate hydrolysis and fermentation was 17.1 g/L at a pH of 4.8 and 150 rpm with 1X107 Cel/mL of Saccharomyces cerevisiae and an enzymatic loading of 25 FPU/g of total carbohydrates. When comparing the results obtained with literature data, it is concluded that this procedure is suitable to exploit the lignocellulosic wastes from the Indigenous Community of San Juan Nuevo Parangaricutiro, Michoacán, Mexico. |
| first_indexed | 2024-12-21T04:40:14Z |
| format | Article |
| id | doaj.art-8eefec34f953400ba9b03378f0f9c45e |
| institution | Directory Open Access Journal |
| issn | 1930-2126 1930-2126 |
| language | English |
| last_indexed | 2024-12-21T04:40:14Z |
| publishDate | 2016-12-01 |
| publisher | North Carolina State University |
| record_format | Article |
| series | BioResources |
| spelling | doaj.art-8eefec34f953400ba9b03378f0f9c45e2022-12-21T19:15:44ZengNorth Carolina State UniversityBioResources1930-21261930-21262016-12-011211052106310.15376/biores.12.1.1052-1063Simultaneous Saccharification and Fermentation of Pine Sawdust (Pinus pseudostrobus L.) Pretreated with Nitric Acid and Sodium Hydroxide for Bioethanol ProductionJuan Carlos Farías-Sánchez0Ulises Velázquez-Valadez1María Guadalupe Pineda-Pimentel2Javier López-Miranda3Agustín Jaime Castro-Montoya4Artemio Carrillo-Parra5Alfonso Vargas-Santillán6José Guadalupe Rutiaga-Quiñones7Universidad Michoacana de San Nicolas de Hidalgo; MexicoUniversidad Michoacana de San Nicolas de Hidalgo; MexicoUniversidad Michoacana de San Nicolas de Hidalgo; MexicoInstituto Tecnológico de Durango; MexicoUniversidad Michoacana de San Nicolas de Hidalgo; Mexico Facultad de Ciencias Forestales; MexicoUniversidad Michoacana de San Nicolas de Hidalgo; MexicoUniversidad Michoacana de San Nicolas de Hidalgo; MexicoWith the purpose of taking advantage of pine sawdust residue coming from a sawmill located in Michoacán, México, a pretreatment with nitric acid and sodium hydroxide was performed. Also, the production of bioethanol by enzymatic hydrolysis was investigated. Using a response surface method, the intermediate points for the optimal HNO3 concentration were determined. Results showed that using HNO3 as a pretreatment leads to higher ethanol yields at an optimal concentration of 10.90% HNO3. After a 30-min pretreatment with 10.90% HNO3 at 114.32 °C, followed by 1% NaOH and enzymatic hydrolysis performed in shaker at a pH of 4.8 and 150 rpm for 72 h, with an enzyme loading of 25 FPU/g of total carbohydrates, the reducing sugars concentration was 99.2% (conversion of polysaccharides to monomers). On the other hand, the ethanol yield obtained from the simultaneous saccharification and fermentation treatment was 15.0 g/L, and the separate hydrolysis and fermentation was 17.1 g/L at a pH of 4.8 and 150 rpm with 1X107 Cel/mL of Saccharomyces cerevisiae and an enzymatic loading of 25 FPU/g of total carbohydrates. When comparing the results obtained with literature data, it is concluded that this procedure is suitable to exploit the lignocellulosic wastes from the Indigenous Community of San Juan Nuevo Parangaricutiro, Michoacán, Mexico.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_12_1_1052_Farias_Sanchez_Saccharification_Fermentation_Pine_SawdustReducing sugars; Enzymatic hydrolysis; Pine sawdust; Pinus pseudostrobus |
| spellingShingle | Juan Carlos Farías-Sánchez Ulises Velázquez-Valadez María Guadalupe Pineda-Pimentel Javier López-Miranda Agustín Jaime Castro-Montoya Artemio Carrillo-Parra Alfonso Vargas-Santillán José Guadalupe Rutiaga-Quiñones Simultaneous Saccharification and Fermentation of Pine Sawdust (Pinus pseudostrobus L.) Pretreated with Nitric Acid and Sodium Hydroxide for Bioethanol Production BioResources Reducing sugars; Enzymatic hydrolysis; Pine sawdust; Pinus pseudostrobus |
| title | Simultaneous Saccharification and Fermentation of Pine Sawdust (Pinus pseudostrobus L.) Pretreated with Nitric Acid and Sodium Hydroxide for Bioethanol Production |
| title_full | Simultaneous Saccharification and Fermentation of Pine Sawdust (Pinus pseudostrobus L.) Pretreated with Nitric Acid and Sodium Hydroxide for Bioethanol Production |
| title_fullStr | Simultaneous Saccharification and Fermentation of Pine Sawdust (Pinus pseudostrobus L.) Pretreated with Nitric Acid and Sodium Hydroxide for Bioethanol Production |
| title_full_unstemmed | Simultaneous Saccharification and Fermentation of Pine Sawdust (Pinus pseudostrobus L.) Pretreated with Nitric Acid and Sodium Hydroxide for Bioethanol Production |
| title_short | Simultaneous Saccharification and Fermentation of Pine Sawdust (Pinus pseudostrobus L.) Pretreated with Nitric Acid and Sodium Hydroxide for Bioethanol Production |
| title_sort | simultaneous saccharification and fermentation of pine sawdust pinus pseudostrobus l pretreated with nitric acid and sodium hydroxide for bioethanol production |
| topic | Reducing sugars; Enzymatic hydrolysis; Pine sawdust; Pinus pseudostrobus |
| url | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_12_1_1052_Farias_Sanchez_Saccharification_Fermentation_Pine_Sawdust |
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