Comparative proteomic analyses of resistant and susceptible crucian carp to Gyrodactylus kobayashii infection
Gyrodactylid monogeneans are common parasites of teleost fishes characterized by high host specificity. Host specificity and its underlying mechanisms of monogenean have been always a fascinating issue that has attracted considerable attention from researchers. However, the molecular mechanism respo...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-08-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513422002174 |
| _version_ | 1811314991450554368 |
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| author | Shun Zhou Xiao Jin Qiuhong Yang Jing Dong Yongtao Liu Ning Xu Yibin Yang Xiaohui Ai |
| author_facet | Shun Zhou Xiao Jin Qiuhong Yang Jing Dong Yongtao Liu Ning Xu Yibin Yang Xiaohui Ai |
| author_sort | Shun Zhou |
| collection | DOAJ |
| description | Gyrodactylid monogeneans are common parasites of teleost fishes characterized by high host specificity. Host specificity and its underlying mechanisms of monogenean have been always a fascinating issue that has attracted considerable attention from researchers. However, the molecular mechanism responsible for the host specificity of monogenean is still not yet fully understood. In this study, the susceptibility to Gyrodactylus kobayashii of different species or varieties in the genus Carassius was investigated. Furthermore, the proteome changes in the skin mucus of resistant and susceptible fish were analyzed by Tandem Mass Tags (TMT) quantitative proteomics method. The experimental results indicated that G. kobayashii was found in 12 varieties of goldfish and ChangFeng C. auratus gibelio with different degrees of infection percentage, while no G. kobayashii was found in C. auratus gibelio var. CAS Ⅴ. Population dynamics of G. kobayashii on red white common goldfish and C. auratus gibelio var. CAS Ⅴ showed distinct trends in the in vivo infection experiment. These results demonstrated C. auratus gibelio var. CAS Ⅴ is a resistant fish to G. kobayashii. Proteomic analysis revealed that 41 differentially expressed proteins (DEPs), including 25 up-regulated and 16 down-regulated proteins, were identified. Bioinformatics analysis indicated these DEPs were involved in multiple biological functions including immunity, metabolism, and signal transduction. Among them, some immune-related proteins, such as cadherin-1, serotransferrin, and glutathione transferase in the fish mucus might be potential determinants of the host susceptibility to G. kobayashii. Also, some structural and energy metabolism-related DEPs, such as small muscular protein, keratin and vitelline membrane outer layer protein served as a source of nutrition and might also affect host susceptibility. Overall, this study not only provided systematic protein information of crucian carp skin mucus but also identified some potential candidate proteins that might be related to host susceptibility to G. kobayashii. |
| first_indexed | 2024-04-13T11:22:24Z |
| format | Article |
| id | doaj.art-8ef17e684eae45b1b8d16a9249aa05eb |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-04-13T11:22:24Z |
| publishDate | 2022-08-01 |
| publisher | Elsevier |
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| series | Aquaculture Reports |
| spelling | doaj.art-8ef17e684eae45b1b8d16a9249aa05eb2022-12-22T02:48:47ZengElsevierAquaculture Reports2352-51342022-08-0125101221Comparative proteomic analyses of resistant and susceptible crucian carp to Gyrodactylus kobayashii infectionShun Zhou0Xiao Jin1Qiuhong Yang2Jing Dong3Yongtao Liu4Ning Xu5Yibin Yang6Xiaohui Ai7Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China; Key Laboratory of Control of Quality and Safety for Aquatic Products, Ministry of Agriculture, Beijing 100071, ChinaKey Laboratory of Aquaculture Disease Control, Ministry of Agriculture, and State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China; Key Laboratory of Control of Quality and Safety for Aquatic Products, Ministry of Agriculture, Beijing 100071, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China; Key Laboratory of Control of Quality and Safety for Aquatic Products, Ministry of Agriculture, Beijing 100071, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China; Key Laboratory of Control of Quality and Safety for Aquatic Products, Ministry of Agriculture, Beijing 100071, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China; Key Laboratory of Control of Quality and Safety for Aquatic Products, Ministry of Agriculture, Beijing 100071, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China; Key Laboratory of Control of Quality and Safety for Aquatic Products, Ministry of Agriculture, Beijing 100071, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China; Key Laboratory of Control of Quality and Safety for Aquatic Products, Ministry of Agriculture, Beijing 100071, China; Correspondence to: Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, No. 8 Wuda Park Road 1, Wuhan 430223, Hubei Province, China.Gyrodactylid monogeneans are common parasites of teleost fishes characterized by high host specificity. Host specificity and its underlying mechanisms of monogenean have been always a fascinating issue that has attracted considerable attention from researchers. However, the molecular mechanism responsible for the host specificity of monogenean is still not yet fully understood. In this study, the susceptibility to Gyrodactylus kobayashii of different species or varieties in the genus Carassius was investigated. Furthermore, the proteome changes in the skin mucus of resistant and susceptible fish were analyzed by Tandem Mass Tags (TMT) quantitative proteomics method. The experimental results indicated that G. kobayashii was found in 12 varieties of goldfish and ChangFeng C. auratus gibelio with different degrees of infection percentage, while no G. kobayashii was found in C. auratus gibelio var. CAS Ⅴ. Population dynamics of G. kobayashii on red white common goldfish and C. auratus gibelio var. CAS Ⅴ showed distinct trends in the in vivo infection experiment. These results demonstrated C. auratus gibelio var. CAS Ⅴ is a resistant fish to G. kobayashii. Proteomic analysis revealed that 41 differentially expressed proteins (DEPs), including 25 up-regulated and 16 down-regulated proteins, were identified. Bioinformatics analysis indicated these DEPs were involved in multiple biological functions including immunity, metabolism, and signal transduction. Among them, some immune-related proteins, such as cadherin-1, serotransferrin, and glutathione transferase in the fish mucus might be potential determinants of the host susceptibility to G. kobayashii. Also, some structural and energy metabolism-related DEPs, such as small muscular protein, keratin and vitelline membrane outer layer protein served as a source of nutrition and might also affect host susceptibility. Overall, this study not only provided systematic protein information of crucian carp skin mucus but also identified some potential candidate proteins that might be related to host susceptibility to G. kobayashii.http://www.sciencedirect.com/science/article/pii/S2352513422002174Host susceptibilityGyrodactylus kobayashiiProteomicsSkin mucusGoldfishGibel carp |
| spellingShingle | Shun Zhou Xiao Jin Qiuhong Yang Jing Dong Yongtao Liu Ning Xu Yibin Yang Xiaohui Ai Comparative proteomic analyses of resistant and susceptible crucian carp to Gyrodactylus kobayashii infection Aquaculture Reports Host susceptibility Gyrodactylus kobayashii Proteomics Skin mucus Goldfish Gibel carp |
| title | Comparative proteomic analyses of resistant and susceptible crucian carp to Gyrodactylus kobayashii infection |
| title_full | Comparative proteomic analyses of resistant and susceptible crucian carp to Gyrodactylus kobayashii infection |
| title_fullStr | Comparative proteomic analyses of resistant and susceptible crucian carp to Gyrodactylus kobayashii infection |
| title_full_unstemmed | Comparative proteomic analyses of resistant and susceptible crucian carp to Gyrodactylus kobayashii infection |
| title_short | Comparative proteomic analyses of resistant and susceptible crucian carp to Gyrodactylus kobayashii infection |
| title_sort | comparative proteomic analyses of resistant and susceptible crucian carp to gyrodactylus kobayashii infection |
| topic | Host susceptibility Gyrodactylus kobayashii Proteomics Skin mucus Goldfish Gibel carp |
| url | http://www.sciencedirect.com/science/article/pii/S2352513422002174 |
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