Temperature- and Nutrients-Induced Phenotypic Changes of Antarctic Green Snow Bacteria Probed by High-Throughput FTIR Spectroscopy
Temperature fluctuations and nutrient composition are the main parameters influencing green snow microbiome. In this study we investigated the influence of temperature and nutrient conditions on the growth and cellular chemical profile of bacteria isolated from green snow. Chemical profiling of the...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-06-01
|
| Series: | Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-7737/11/6/890 |
| _version_ | 1797489807899754496 |
|---|---|
| author | Margarita Smirnova Valeria Tafintseva Achim Kohler Uladzislau Miamin Volha Shapaval |
| author_facet | Margarita Smirnova Valeria Tafintseva Achim Kohler Uladzislau Miamin Volha Shapaval |
| author_sort | Margarita Smirnova |
| collection | DOAJ |
| description | Temperature fluctuations and nutrient composition are the main parameters influencing green snow microbiome. In this study we investigated the influence of temperature and nutrient conditions on the growth and cellular chemical profile of bacteria isolated from green snow. Chemical profiling of the green snow bacteria was done by high-throughput FTIR spectroscopy combined with multivariate data analysis. We showed that temperature and nutrients fluctuations strongly affect growth ability and chemical profile of the green snow bacteria. The size of colonies for green snow bacteria grown at higher (25 °C) and lower (4 °C and 10 °C) than optimal temperature (18 °C) was smaller. All isolates grew on rich medium, and only 19 isolates were able to grow on synthetic minimal media. Lipid and mixed spectral regions showed to be phylogeny related. FTIR fingerprinting indicates that lipids are often affected by the temperature fluctuations. Growth on different media resulted in the change of the whole chemical profile, where lipids showed to be more affected than proteins and polysaccharides. Correlation analysis showed that nutrient composition is clearly strongly influencing chemical changes in the cells, followed by temperature. |
| first_indexed | 2024-03-10T00:22:55Z |
| format | Article |
| id | doaj.art-8ef4d07ec1a44ca79c9e7b057afd680d |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T00:22:55Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-8ef4d07ec1a44ca79c9e7b057afd680d2023-11-23T15:40:00ZengMDPI AGBiology2079-77372022-06-0111689010.3390/biology11060890Temperature- and Nutrients-Induced Phenotypic Changes of Antarctic Green Snow Bacteria Probed by High-Throughput FTIR SpectroscopyMargarita Smirnova0Valeria Tafintseva1Achim Kohler2Uladzislau Miamin3Volha Shapaval4Faculty of Science and Technology, Norwegian University of Life Sciences, 1432 Aas, NorwayFaculty of Science and Technology, Norwegian University of Life Sciences, 1432 Aas, NorwayFaculty of Science and Technology, Norwegian University of Life Sciences, 1432 Aas, NorwayFaculty of Biology, Belarussian State University, 220030 Minsk, BelarusFaculty of Science and Technology, Norwegian University of Life Sciences, 1432 Aas, NorwayTemperature fluctuations and nutrient composition are the main parameters influencing green snow microbiome. In this study we investigated the influence of temperature and nutrient conditions on the growth and cellular chemical profile of bacteria isolated from green snow. Chemical profiling of the green snow bacteria was done by high-throughput FTIR spectroscopy combined with multivariate data analysis. We showed that temperature and nutrients fluctuations strongly affect growth ability and chemical profile of the green snow bacteria. The size of colonies for green snow bacteria grown at higher (25 °C) and lower (4 °C and 10 °C) than optimal temperature (18 °C) was smaller. All isolates grew on rich medium, and only 19 isolates were able to grow on synthetic minimal media. Lipid and mixed spectral regions showed to be phylogeny related. FTIR fingerprinting indicates that lipids are often affected by the temperature fluctuations. Growth on different media resulted in the change of the whole chemical profile, where lipids showed to be more affected than proteins and polysaccharides. Correlation analysis showed that nutrient composition is clearly strongly influencing chemical changes in the cells, followed by temperature.https://www.mdpi.com/2079-7737/11/6/890green snow bacteriacellular chemical profileFTIR spectroscopymultivariate data analysis |
| spellingShingle | Margarita Smirnova Valeria Tafintseva Achim Kohler Uladzislau Miamin Volha Shapaval Temperature- and Nutrients-Induced Phenotypic Changes of Antarctic Green Snow Bacteria Probed by High-Throughput FTIR Spectroscopy Biology green snow bacteria cellular chemical profile FTIR spectroscopy multivariate data analysis |
| title | Temperature- and Nutrients-Induced Phenotypic Changes of Antarctic Green Snow Bacteria Probed by High-Throughput FTIR Spectroscopy |
| title_full | Temperature- and Nutrients-Induced Phenotypic Changes of Antarctic Green Snow Bacteria Probed by High-Throughput FTIR Spectroscopy |
| title_fullStr | Temperature- and Nutrients-Induced Phenotypic Changes of Antarctic Green Snow Bacteria Probed by High-Throughput FTIR Spectroscopy |
| title_full_unstemmed | Temperature- and Nutrients-Induced Phenotypic Changes of Antarctic Green Snow Bacteria Probed by High-Throughput FTIR Spectroscopy |
| title_short | Temperature- and Nutrients-Induced Phenotypic Changes of Antarctic Green Snow Bacteria Probed by High-Throughput FTIR Spectroscopy |
| title_sort | temperature and nutrients induced phenotypic changes of antarctic green snow bacteria probed by high throughput ftir spectroscopy |
| topic | green snow bacteria cellular chemical profile FTIR spectroscopy multivariate data analysis |
| url | https://www.mdpi.com/2079-7737/11/6/890 |
| work_keys_str_mv | AT margaritasmirnova temperatureandnutrientsinducedphenotypicchangesofantarcticgreensnowbacteriaprobedbyhighthroughputftirspectroscopy AT valeriatafintseva temperatureandnutrientsinducedphenotypicchangesofantarcticgreensnowbacteriaprobedbyhighthroughputftirspectroscopy AT achimkohler temperatureandnutrientsinducedphenotypicchangesofantarcticgreensnowbacteriaprobedbyhighthroughputftirspectroscopy AT uladzislaumiamin temperatureandnutrientsinducedphenotypicchangesofantarcticgreensnowbacteriaprobedbyhighthroughputftirspectroscopy AT volhashapaval temperatureandnutrientsinducedphenotypicchangesofantarcticgreensnowbacteriaprobedbyhighthroughputftirspectroscopy |