Histochemical Evaluation of Mucin-Secreting Cell Activity in the Stomach of the Wistar Rat
Mucin plays a very important role in the physiological function of the stomach in all species, but the representation of mucin-secreting cells differs from species to species. The aim of this study was to highlight these cells in the stomach of the Wistar rat. For histological investigations, fragm...
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| Format: | Article |
| Language: | English |
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AcademicPres
2022-11-01
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| Series: | Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Veterinary Medicine |
| Online Access: | https://journals.usamvcluj.ro/index.php/veterinary/article/view/14381 |
| _version_ | 1827093589134409728 |
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| author | Elena HUȚANU Aurel DAMIAN Ioana RAȚIU Vasile RUS Maria-Cătălina MATEI-LAȚIU Cristian MARTONOS Adrian Florin GAL |
| author_facet | Elena HUȚANU Aurel DAMIAN Ioana RAȚIU Vasile RUS Maria-Cătălina MATEI-LAȚIU Cristian MARTONOS Adrian Florin GAL |
| author_sort | Elena HUȚANU |
| collection | DOAJ |
| description |
Mucin plays a very important role in the physiological function of the stomach in all species, but the representation of mucin-secreting cells differs from species to species. The aim of this study was to highlight these cells in the stomach of the Wistar rat. For histological investigations, fragments of the stomach from the three main regions (cardiac, fundic and pyloric) were harvested from four Wistar rats and further stained with PAS and Alcian blue methods. The histochemical results showed the presence of mucin-secreting cells in the examined tissues. An intense PAS positive reaction was found in the surface epithelium cells of the gastric mucosa and in the cells lining the gastric crypts in all the three segments. In the deep glandular structures, the cells of the cardiac and fundic glands are PAS negative, while those of the pyloric glands are all PAS positive. For Alcian blue reaction, the cells lining the gastric mucosa and the crypts in the three regions of the stomach were negative. In the glands, the cells of the cardiac and fundic glands were Alcian negative while in the pyloric glands, a limited number of cells arranged in the deep third of the glands showed a weak positive Alcian reaction. In conclusion, the vast majority of glandular cells in the rat's stomach do not synthesize acidic mucins, except for cells in the deep part of the pyloric glands, which synthesize small amounts of such mucins.
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| issn | 1843-5270 1843-5378 |
| language | English |
| last_indexed | 2025-03-20T06:30:29Z |
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| series | Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Veterinary Medicine |
| spelling | doaj.art-8ef6acf094ba4e1ba576db1b85647b1d2024-10-02T09:25:40ZengAcademicPresBulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Veterinary Medicine1843-52701843-53782022-11-0179210.15835/buasvmcn-vm:2022.001914381Histochemical Evaluation of Mucin-Secreting Cell Activity in the Stomach of the Wistar RatElena HUȚANU0Aurel DAMIAN1Ioana RAȚIU2Vasile RUS3Maria-Cătălina MATEI-LAȚIU4Cristian MARTONOS5Adrian Florin GAL6Faculty of Veterinary Medicine, University of Agricultural Scienes and Veterinary Medicine Cluj-Napoca, Mănăștur Street, no 3-5, 400372, Cluj-NapocaUniversity of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Mănăștur St. no 3-5, 400372, Cluj-Napoca, ClujUniversity of Oradea, 410073 OradeaUniversity of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Mănăștur Street, no 3-5, 400372, Cluj-NapocaUniversity of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Mănăștur Street, no 3-5, 400372, Cluj-NapocaUniversity of Agricultural Scienes and Veterinary Medicine Cluj-Napoca, Mănăștur Street, no 3-5, 400372, Cluj-NapocaUniversity of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Mănăștur Street, no 3-5, 400372, Cluj-Napoca Mucin plays a very important role in the physiological function of the stomach in all species, but the representation of mucin-secreting cells differs from species to species. The aim of this study was to highlight these cells in the stomach of the Wistar rat. For histological investigations, fragments of the stomach from the three main regions (cardiac, fundic and pyloric) were harvested from four Wistar rats and further stained with PAS and Alcian blue methods. The histochemical results showed the presence of mucin-secreting cells in the examined tissues. An intense PAS positive reaction was found in the surface epithelium cells of the gastric mucosa and in the cells lining the gastric crypts in all the three segments. In the deep glandular structures, the cells of the cardiac and fundic glands are PAS negative, while those of the pyloric glands are all PAS positive. For Alcian blue reaction, the cells lining the gastric mucosa and the crypts in the three regions of the stomach were negative. In the glands, the cells of the cardiac and fundic glands were Alcian negative while in the pyloric glands, a limited number of cells arranged in the deep third of the glands showed a weak positive Alcian reaction. In conclusion, the vast majority of glandular cells in the rat's stomach do not synthesize acidic mucins, except for cells in the deep part of the pyloric glands, which synthesize small amounts of such mucins. https://journals.usamvcluj.ro/index.php/veterinary/article/view/14381 |
| spellingShingle | Elena HUȚANU Aurel DAMIAN Ioana RAȚIU Vasile RUS Maria-Cătălina MATEI-LAȚIU Cristian MARTONOS Adrian Florin GAL Histochemical Evaluation of Mucin-Secreting Cell Activity in the Stomach of the Wistar Rat Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Veterinary Medicine |
| title | Histochemical Evaluation of Mucin-Secreting Cell Activity in the Stomach of the Wistar Rat |
| title_full | Histochemical Evaluation of Mucin-Secreting Cell Activity in the Stomach of the Wistar Rat |
| title_fullStr | Histochemical Evaluation of Mucin-Secreting Cell Activity in the Stomach of the Wistar Rat |
| title_full_unstemmed | Histochemical Evaluation of Mucin-Secreting Cell Activity in the Stomach of the Wistar Rat |
| title_short | Histochemical Evaluation of Mucin-Secreting Cell Activity in the Stomach of the Wistar Rat |
| title_sort | histochemical evaluation of mucin secreting cell activity in the stomach of the wistar rat |
| url | https://journals.usamvcluj.ro/index.php/veterinary/article/view/14381 |
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