Long-Term Stability of Blood Serum Biomarkers in Traumatic Brain Injury: A Feasibility Study
Few studies on traumatic brain injury (TBI) have investigated the stability of blood serum biomarkers after long-term storage at low temperatures. In the current feasibility study we analyzed acute phase serum samples from patients with mild TBI as well as patients with moderate and severe TBI that...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Neurology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fneur.2022.877050/full |
| _version_ | 1811246828133285888 |
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| author | Harm Jan van der Horn Koen Visser Johan Bijzet Pieter Vos Joukje van der Naalt Bram Jacobs |
| author_facet | Harm Jan van der Horn Koen Visser Johan Bijzet Pieter Vos Joukje van der Naalt Bram Jacobs |
| author_sort | Harm Jan van der Horn |
| collection | DOAJ |
| description | Few studies on traumatic brain injury (TBI) have investigated the stability of blood serum biomarkers after long-term storage at low temperatures. In the current feasibility study we analyzed acute phase serum samples from patients with mild TBI as well as patients with moderate and severe TBI that were collected more than 10 years ago (old samples). We were particularly interested in mild TBI, because injury effects are more subtle in this category as compared to moderate-severe TBI. Therefore, the primary objective was to find out whether several biomarkers were still detectable for these patients. Additionally, we examined whether biomarker levels varied as a function of injury severity. For comparison, we also analyzed samples from an ongoing mTBI cohort (new samples) and healthy controls. Samples were treated with care and were not being subjected to freeze-thaw cycles. We measured concentrations of interleukins (IL6 and 10) and brain specific markers (total tau, UCH-L1, GFAP, and NF-L). No significant differences in biomarker concentrations were found between old and new mild TBI samples. For IL6, IL10, and UCH-L1 higher concentrations were found in moderate and severe TBI as compared to mild TBI. In conclusion, our study shows that long-term storage does not rule out the detection of meaningful biomarker concentrations in patients with TBI, although further research by other laboratories is warranted. |
| first_indexed | 2024-04-12T14:58:58Z |
| format | Article |
| id | doaj.art-8efbc9109fd049cf82a3d949706193e2 |
| institution | Directory Open Access Journal |
| issn | 1664-2295 |
| language | English |
| last_indexed | 2024-04-12T14:58:58Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neurology |
| spelling | doaj.art-8efbc9109fd049cf82a3d949706193e22022-12-22T03:28:07ZengFrontiers Media S.A.Frontiers in Neurology1664-22952022-05-011310.3389/fneur.2022.877050877050Long-Term Stability of Blood Serum Biomarkers in Traumatic Brain Injury: A Feasibility StudyHarm Jan van der Horn0Koen Visser1Johan Bijzet2Pieter Vos3Joukje van der Naalt4Bram Jacobs5Department of Neurology, University of Groningen, University Medical Center Groningen, Groningen, NetherlandsDepartment of Neurology, University of Groningen, University Medical Center Groningen, Groningen, NetherlandsDepartment of Rheumatology and Clinical Immunology, University of Groningen, University Medical Center Groningen, Groningen, NetherlandsDepartment of Neurology, Slingeland Hospital, Doetinchem, NetherlandsDepartment of Neurology, University of Groningen, University Medical Center Groningen, Groningen, NetherlandsDepartment of Neurology, University of Groningen, University Medical Center Groningen, Groningen, NetherlandsFew studies on traumatic brain injury (TBI) have investigated the stability of blood serum biomarkers after long-term storage at low temperatures. In the current feasibility study we analyzed acute phase serum samples from patients with mild TBI as well as patients with moderate and severe TBI that were collected more than 10 years ago (old samples). We were particularly interested in mild TBI, because injury effects are more subtle in this category as compared to moderate-severe TBI. Therefore, the primary objective was to find out whether several biomarkers were still detectable for these patients. Additionally, we examined whether biomarker levels varied as a function of injury severity. For comparison, we also analyzed samples from an ongoing mTBI cohort (new samples) and healthy controls. Samples were treated with care and were not being subjected to freeze-thaw cycles. We measured concentrations of interleukins (IL6 and 10) and brain specific markers (total tau, UCH-L1, GFAP, and NF-L). No significant differences in biomarker concentrations were found between old and new mild TBI samples. For IL6, IL10, and UCH-L1 higher concentrations were found in moderate and severe TBI as compared to mild TBI. In conclusion, our study shows that long-term storage does not rule out the detection of meaningful biomarker concentrations in patients with TBI, although further research by other laboratories is warranted.https://www.frontiersin.org/articles/10.3389/fneur.2022.877050/fullbiomarkersinflammationstabilitytraumatic brain injurylaboratoryconcussion |
| spellingShingle | Harm Jan van der Horn Koen Visser Johan Bijzet Pieter Vos Joukje van der Naalt Bram Jacobs Long-Term Stability of Blood Serum Biomarkers in Traumatic Brain Injury: A Feasibility Study Frontiers in Neurology biomarkers inflammation stability traumatic brain injury laboratory concussion |
| title | Long-Term Stability of Blood Serum Biomarkers in Traumatic Brain Injury: A Feasibility Study |
| title_full | Long-Term Stability of Blood Serum Biomarkers in Traumatic Brain Injury: A Feasibility Study |
| title_fullStr | Long-Term Stability of Blood Serum Biomarkers in Traumatic Brain Injury: A Feasibility Study |
| title_full_unstemmed | Long-Term Stability of Blood Serum Biomarkers in Traumatic Brain Injury: A Feasibility Study |
| title_short | Long-Term Stability of Blood Serum Biomarkers in Traumatic Brain Injury: A Feasibility Study |
| title_sort | long term stability of blood serum biomarkers in traumatic brain injury a feasibility study |
| topic | biomarkers inflammation stability traumatic brain injury laboratory concussion |
| url | https://www.frontiersin.org/articles/10.3389/fneur.2022.877050/full |
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